LOCUS       sequence-88342-e        8918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 400 / 504, Emission: 515 
            / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8918)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 8918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Talin-18
FEATURES             Location/Qualifiers
     source          1..8918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(179..197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      790..799
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
       61 atggccatca ttaaggagtt catgcgcttc aaggtgcaca tggaggggtc cgtgaacggc
      121 cacgtgttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      181 aagctgaagg tgaccaaggg cggccccctg cccttcacct gggacatcct gagccctcag
      241 ttcatgtacg gctccaatgc ctacgtgaag caccccgccg acatccccga ctactttaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga aattcgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtgatgcaga agaagaccat gggctgggag
      481 gccctctccg agcggatgta ccccgaggac ggcgccctga agggcgaggt caagcccaga
      541 gtgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacaac gtcaaccgca agctggacat caccagccac
      661 aacgaggact acaccatcgt ggagcagtac gagagagccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagctag cttcgaattc
      781 gcagaagctg ccaccatggt tgcgctttcg ctgaagatta gcattgggaa tgtggtgaag
      841 acgatgcaat ttgagccatc taccatggtg tacgacgcct gccgcatgat tcgtgagcgg
      901 atcccagagg ccctggctgg ccctcccaac gactttgggc tctttctgtc agatgatgac
      961 cccaaaaaag gcatctggct agaggctggg aaagctttgg actactacat gctccgaaat
     1021 ggggacacca tggagtacag aaaaaagcag agacccctga agatacggat gttagacgga
     1081 acggtgaaga ctatcatggt ggatgactcc aagaccgtca cagacatgct catgaccatt
     1141 tgtgcccgaa ttggtatcac caaccatgat gagtattcac tggttcgaga gctgatggaa
     1201 gaaaagaaag atgaggggac agggaccctg agaaaagaca agaccctact gcgagatgaa
     1261 aagaagatgg agaagctaaa gcagaagttg cacacagacg atgagttgaa ctggctggac
     1321 catgggcgga cactgaggga acagggagtg gaagagcatg agacgttgct gctgcggaga
     1381 aagttcttct actcagacca gaatgtggat tcccgagacc ctgtacagct gaaccttctc
     1441 tatgtgcagg cacgagatga catcctgaat ggctcccatc ctgtctcctt tgacaaggcc
     1501 tgtgaatttg caggcttcca gtgccagatc cagtttggac ctcacaatga acagaagcac
     1561 aaggctggct tccttgacct gaaggacttc ctgcccaagg agtatgtgaa gcagaaggga
     1621 gagcgtaaga tctttcaggc acacaagaat tgtgggcaga tgagtgaaat tgaggccaag
     1681 gtacgctacg tgaagctggc ccgttccctc aagacttacg gtgtctcctt cttcctagtc
     1741 aaggaaaaga tgaaggggaa gaataaactg gtgcccaggc tgttgggcat cactaaggag
     1801 tgtgtgatgc gtgtggatga gaagaccaag gaggtgattc aggaatggag cctcaccaac
     1861 atcaaacgct gggctgcctc tcccaagagc tttactctgg actttggaga ctatcaggat
     1921 ggctactact cagtacagac gacagaaggc gagcagatcg cacagctcat tgctggctac
     1981 atagatatca tccttaagaa gaaaaaaagc aaggaccatt ttgggctgga gggagatgaa
     2041 gagtctacta tgttggagga ctcagtgtct cccaaaaagt caacagtcct tcagcagcag
     2101 tacaaccgag tggggaaagt ggagcacggc tctgtggctc tgccagccat catgcgctct
     2161 ggagcctctg gtcctgagaa tttccaggtg gggagcatgc cacctgccca gcagcagatc
     2221 accagtggcc agatgcaccg aggacacatg ccacctctga cttcagccca gcaggcgctc
     2281 actggaacca ttaactccag catgcaggct gtgcaggctg cccaggccac tctggatgac
     2341 tttgagactc taccccctct tggccaggat gctgcctcca aagcttggcg taagaacaag
     2401 atggatgaat caaagcatga gatccactcc caggtagatg ccatcacagc tggcactgcc
     2461 tctgtggtaa acctgacagc aggagatcct gcagagacag actatacagc agtgggctgt
     2521 gcagtcacca ccatctcttc caacctgacg gagatgtccc gtggggtgaa gctattggct
     2581 gccctgctgg aggatgaagg cggcaatggc cggccccttc tgcaagcagc aaagggcctt
     2641 gcaggggctg tgtcggaact gcttcgcagt gctcagcccg ccagcgctga gcctcgtcag
     2701 aacctgctgc aggcagccgg gaacgtgggc caggccagtg gggagctgtt gcagcaaatt
     2761 ggggaaagtg acactgaccc ccacttccag gatgttctaa tgcagctagc aaacgcagtg
     2821 gcaagtgctg cagccgccct ggtcctcaag gccaagagtg tggcccagcg cacagaggat
     2881 tccgggcttc agacccaagt gattgctgca gctacgcaat gtgctctgtc cacttcccag
     2941 ctggtggcct gtaccaaggt ggtagcacct acaatcagct cacctgtgtg ccaagaacag
     3001 ctagtcgagg ctgggcgact ggtggccaaa gctgtggagg gctgtgtgtc tgcctcgcaa
     3061 gcagctacag aagatggaca gctgcttcga ggggtaggag cagcagccac ggctgtcacc
     3121 caggccctca atgagctgct gcagcacgtg aaggcccacg ccaccggagc tgggcctgcc
     3181 ggtcggtatg accaagccac tgataccatc ctcaccgtca ctgaaaacat cttcagctcc
     3241 atgggtgatg ctggggagat ggtgcgccag gcccgcatcc tggcccaagc cacctcagac
     3301 ctggtcaatg ctatcaaggc cgatgctgag ggggagagtg atctggagaa ctctagaaag
     3361 ctcctgagtg ctgccaagat cctcgctgat gccaccgcca agatggtgga ggcggccaag
     3421 ggagcagccg cccaccctga cagtgaggaa cagcagcagc gactgcgtga agcagctgag
     3481 gggcttcgca tggccaccaa tgcagctgcg cagaacgcca tcaagaagaa gttggtgcag
     3541 cgcctggagc atgcagccaa gcaagctgca gcctctgcaa cacagaccat tgctgcagcc
     3601 caacatgcag cctctgcccc caaggcctct gcgggccccc agcccctact ggtgcagagc
     3661 tgtaaggccg tggcagagca gattccgttg ctggtgcagg gtgtccgagg aagtcaagct
     3721 cagcctgaca gccctagtgc tcagctcgcc ctcattgctg ccagccagag cttcctgcag
     3781 ccaggtggga agatggtggc agcggcaaaa gcctcagtgc caacaattca ggaccaggct
     3841 tcagcgatgc agctgagtca gtgcgcaaag aacctaggca ctgccctggc cgagctgcgc
     3901 actgctgctc agaaggctca ggaggcatgt ggacctttgg agatggattc tgcactgagt
     3961 gttgtacaaa atctagagaa agacctacag gaaataaaag cagcagctcg agatggcaag
     4021 cttaagccct tacctgggga aacaatggag aagtgtaccc aagatcttgg caacagcacc
     4081 aaagcggtga gctctgccat cgccaagctg ctgggggaga ttgcccaggg caacgagaac
     4141 tatgcaggta ttgcagctcg ggatgtagct ggtggactaa ggtcactagc ccaggctgcg
     4201 cggggtgtgg ctgcgctgac atcagatcct gcagtgcagg ccattgtgct tgacacagcc
     4261 agcgacgttc tggacaaggc cagcagcctc attgaagagg caaaaaaagc atctggacac
     4321 ccaggggacc cagaaagcca gcaaaggctt gctcaggtag ctaaagcagt gacccaagcc
     4381 ctgaaccgct gtgtcagctg cctgcctggc caaagagacg tggataatgc cctacgagcc
     4441 gttggagatg ccagcaagcg actcctgagt gacttgcttc ctccaagcac ggggacattt
     4501 caagaagcac agagccgatt gaatgaagct gcagctgggt taaatcaggc cgccacagaa
     4561 ctggtgcaag cctcccgagg aactcctcag gacctggctc gggcctccgg ccgatttgga
     4621 caggacttca gcaccttcct ggaagctggt gtggagatgg ccggacaggc cccgagccag
     4681 gaggaccgag cccaggtggt gtccaacctg aagggcatat ccatgtcttc aagcaaactt
     4741 ctccttgccg ctaaggcctt gtccacagac cctgcttctc ccaacctcaa gagtcagctg
     4801 gctgcggctg cccgggcagt gacggacagc atcaaccagc tcatcaccat gtgcacccag
     4861 caggcacctg gccagaagga gtgtgacaat gcacttcggc agctagagac agtccgagaa
     4921 ctcctggaga atccagtcca gcccatcaac gacatgtcct acttcggttg cttggacagt
     4981 gtcatggaga actccaaggt cctaggtgag gccatgactg gcatctccca aaatgccaag
     5041 aatggaaatc tgccggagtt tggagatgcc attgccacag cctccaaggc tctctgtggc
     5101 ttcacggagg cagccgcaca ggcagcatat ctagttggtg tctctgaccc caacagccaa
     5161 gctggacagc aaggactggt ggaacccaca cagtttgccc gtgcaaacca ggcaattcag
     5221 atggcctgtc agagtctggg ggagcctggc tgtacccagg cccaggtgtt atctgcagcc
     5281 actattgtag ccaaacacac atctgcattg tgtaacagct gtcgcctggc ttccgctagg
     5341 actgccaatc ccactgccaa gcgccagttt gtacagtcag ccaaggaggt ggccaacagt
     5401 acagccaatc ttgtcaagac catcaaggca ctagatgggg acttcacaga agagaaccgt
     5461 gcccagtgcc gagcagccac agcccctctg ctggaagctg tggataacct gagtgccttt
     5521 gcctccaacc ctgagttctc cagcgtccct gcccagatca gccctgaggg cagagcagcc
     5581 atggagccca ttgtaatctc tgctaagaca atgttggaga gtgctggagg gctcatccag
     5641 acagctcggg cgttagcagt caatccccga gaccccccac gttggtctgt gctagctggc
     5701 cactctcgga ctgtctcaga ttccatcaag aaacttatta caagcatgag ggacaaagcc
     5761 ccagggcagc tggagtgtga gacagccatt gcggctctga acagctgctt gcgggaccta
     5821 gatcaggctt cgcttgccgc tgtcagccag cagcttgctc cccgtgaagg aatctctcaa
     5881 gaggctttgc acacccagat gctcactgca gtgcaagaaa tttctcatct cattgagccg
     5941 ctggccagcg ctgctcgggc tgaagcctcc cagctgggac acaaggtgtc ccaaatggcc
     6001 cagtactttg aaccactcac cctggctgca gtgggtgctg cgtctaagac cctgagccac
     6061 ccgcagcaga tggcacttct ggaccagact aaaacgttgg cagagtctgc cttgcagttg
     6121 ctatatactg ccaaggaggc tggtggtaac cccaagcaag cagcacacac ccaggaagcc
     6181 ctggaggagg ctgtgcagat gatgacagag gccgtagaag acctgacgac gaccctcaac
     6241 gaagcagcca gtgctgcagg agtcgttggc ggcatggtgg actctatcac tcaggccatc
     6301 aaccagctag atgaaggacc tatgggtgac ccagaaggct cattcgtaga ttaccagaca
     6361 accatggtga ggacagccaa ggccattgct gtcactgttc aggagatggt aaccaagtca
     6421 aacaccagcc ctgaagagct aggccctctt gccaaccagc tgaccagtga ctatggccga
     6481 ctggcctcac aagccaagcc tgcagctgtg gctgctgaaa atgaagagat aggcgctcac
     6541 atcaaacacc gagtacagga gctgggccat ggctgctctg ctctggtcac caaggcaggt
     6601 gccttgcagt gtagccccag tgatgtctac accaagaagg agctcataga gtgtgcccgc
     6661 agagtgtcag agaaggtctc ccatgttctg gctgcactcc aggctgggaa tcgtggtacc
     6721 caggcctgca ttacagcagc cagtgctgtg tctggtatca ttgctgacct cgacaccacc
     6781 atcatgttcg ctactgctgg cacacttaac cgtgagggtg ctgaaacttt tgctgaccac
     6841 cgggagggta tcttaaagac agcaaaggtt cttgtggagg acaccaaggt cctagtgcag
     6901 aatgcagctg ggagccagga gaagttggca caagccgccc agtcctccgt ggccaccatt
     6961 acccgcctcg ctgatgtggt caagctcggt gcagccagcc taggagccga agaccctgaa
     7021 actcaggtgg tgctgatcaa tgcagtaaag gacgtagcca aggccctggg tgacctcatc
     7081 agcgctacga aggctgcagc gggcaaagtt ggggatgacc ctgcagtgtg gcagctcaag
     7141 aactctgcca aggtgatggt gaccaatgtg acatcattgc tcaagacagt gaaggctgtg
     7201 gaagatgagg ccaccaaagg cacacgggcc ctagaggcaa ccacagagca catacgtcag
     7261 gaactggcgg tcttctgttc cccagagcca cctgccaaga cctctacccc tgaagacttc
     7321 atccgaatga ccaagggtat tactatggca acagccaaag ccgttgctgc tggcaattcc
     7381 tgtcgacagg aagatgtcat tgccacagcc aatctgagcc gacgggctat tgcggacatg
     7441 cttcgggctt gcaaggaagc agctttccac ccagaagtgg cgcctgatgt acggctccga
     7501 gccctgcact atggccggga gtgtgccaat ggttacctgg aactgctgga ccacgtgctg
     7561 ctgacccttc agaagccaaa cccagacctg aagcagcagc taactggaca ctcaaagcgg
     7621 gttgctggct ctgtgactga gctcatccag gctgctgaag ccatgaaagg aacagagtgg
     7681 gtggacccag aggaccctac tgtcattgct gagaatgagc tcttgggagc cgcagccgcc
     7741 atcgaggctg cagccaagaa gctggagcag ctgaagccca gggccaaacc caaggaggcg
     7801 gatgagtcct tgaactttga ggaacaaatc ctagaagctg ccaagtccat cgctgcagcc
     7861 accagtgcac tggtaaaggc tgcgtcagca gcccagaggg agctggtggc tcaaggaaag
     7921 gtgggcgcca ttccagccaa tgcactggat gatgggcagt ggtcgcaggg cctcatttcc
     7981 gctgcccgta tggtggctgc ggccaccaac aatctgtgtg aagcagctaa cgcagctgtc
     8041 cagggccatg ctagccagga gaaactcatt tcctcagcca agcaagtagc tgcctccaca
     8101 gctcagctcc tggtagcttg caaggtcaaa gcagatcagg actctgaggc aatgaaacgg
     8161 ctccaggctg ctggcaatgc agtgaagagg gcctcagata acctggtaaa ggcggcccag
     8221 aaagctgcag ccttcgaaga ccaggagaat gagacggtgg tggtgaagga gaaaatggtt
     8281 gggggcattg cccagattat cgcagcacag gaagagatgc ttcggaagga acgagagctg
     8341 gaagaggctc ggaaaaagct cgcccagatc cggcagcagc agtacaagtt cttgccttca
     8401 gagcttcgag acgagcacta aaaaagccct gtgtatttaa tgcagaccca gcccagagac
     8461 tgtgcctgcc actaccaaag ccttctgggc tgctcaggac ccaagctgcc caaccccagc
     8521 actcccccaa agtgcctgcc aaaccctggg cctggccctg cccagtccca ctgcaagccg
     8581 tgtcctctcc ctgactccca agtgcctttg caccctaggg cccctaagtg cctgcccccc
     8641 tccagagtat taacgctcca agagtattat taatgctgcc gtacctcagt ttgaacctgc
     8701 cagggcccca gctgctccag cctgccagca gcttccagcc agtccccacg gccacgtcag
     8761 ctcaactcat ccctttttga tactatatcc cctacccagc tacctatggg gcttgagggt
     8821 tgtaaaccca aacaggtcag actcccggaa ttctgcagtc gacggtaccg cgggcccggg
     8881 atccaccgga tctagataac tgatcataat cagccata
//
