LOCUS       sequence-88344-e        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 400 / 504, Emission: 515 
            / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-VASP-5
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..736
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(174..192)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acggtcagat ccgctagcgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc
       61 catcattaag gagttcatgc gcttcaaggt gcacatggag gggtccgtga acggccacgt
      121 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      181 gaaggtgacc aagggcggcc ccctgccctt cacctgggac atcctgagcc ctcagttcat
      241 gtacggctcc aatgcctacg tgaagcaccc cgccgacatc cccgactact ttaagctgtc
      301 cttccccgag ggcttcaagt gggagcgcgt gatgaaattc gaggacggcg gcgtggtgac
      361 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
      421 caccaacttc ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggccct
      481 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gaggtcaagc ccagagtgaa
      541 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
      601 cgtgcagctg cccggcgcct acaacgtcaa ccgcaagctg gacatcacca gccacaacga
      661 ggactacacc atcgtggagc agtacgagag agccgagggc cgccactcca ccggcggcat
      721 ggacgagctg tacaagtccg gactcagatc tatgagcgag acggtcatct gttccagccg
      781 ggctactgtg atgctttatg atgacagcaa caagcgatgg ctccctgctg gcactggtcc
      841 ccaggccttc agccgcgtcc agatatacca caaccccact gctaactcct tccgagttgt
      901 tggccgcaag atgcagccgg atcagcaggt ggttatcaac tgtgccatca ttcggggtgt
      961 caagtacaat caggccactc ccatcttcca tcagtggcga gatgcccgcc aggtctgggg
     1021 cctcaacttc ggcagcaagg aggacgccat acagtttgca acaggcatgg ccaacgccct
     1081 agaggccttg gaaggaggtg ggcctccccc agccccagca ccccctgcct ggtctgccca
     1141 gaatggtccc tccccagagg agctggaaca acagaaaagg cagccggagc atatggagcg
     1201 ccgggtctcc aatgcaggag gcccacctgc tcccccagct gggggccctc ctccacctcc
     1261 aggacctccc cctcctccag gtcccccccc acccccaggt ctgccctcct caggggtatc
     1321 tggggcaggt catggagcag gggcagcccc accccctgca cccccactcc ctacaacaca
     1381 gggccccaat agtgggggtt ccggggcccc aggcctggct gctgccattg ctggagccaa
     1441 actcaggaaa gtgagcaagc aggaggaggc ctctgggggg cccctggccc ccaaagctga
     1501 gaacagtcga agcactggtg gggggcttat ggaagagatg aacgccatgc tggcccggag
     1561 aagaaaagcc acacaggttg gggagaagcc ccccaaagac gagtcagcca gtgaggagtc
     1621 agaggcccga ctccctgccc agagtgaacc tgtgagaaga ccctgggaga agaacagcac
     1681 aaccttgcca aggatgaagt cgtcctcttc tgtgactacc tccgaggccc acccctcgac
     1741 gccctgctcc agtgatgact ccgacttgga gagggtgaag caggagcttc tggaagaggt
     1801 gcggaaggag ctacagaaaa tgaaagagga aatcatcgaa gtctttgtcc aggagctgag
     1861 gaagcggggt tctccttgag cggccgcgac tctagatcat aatcagccat accacat
//
