LOCUS       sequence-88345-e        2228 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 400 / 504, 
            Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   York AG, Ghitani A, Vaziri A, Davidson MW, Shroff H
  TITLE     Confined activation and subdiffractive localization enables 
            whole-cell PALM with genetically expressed probes.
  JOURNAL   Nat Methods. 2011 Apr;8(4):327-33. doi: 10.1038/nmeth.1571. Epub 
            2011 Feb 13.
  PUBMED    21317909
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1571"; journalName: "Nat Methods"; date: "2011-04"; 
            volume: "8"; issue: "4"; pages: "327-33"
COMMENT     Sequence Label: PA-mCherry1-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2228
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1473..1482
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1479..2189
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1624..1642)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gaccgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattccca
       61 tgtccaccag gtccgtgtcc tcgtcctcct accgcaggat gttcggcggc ccgggcaccg
      121 cgagccggcc gagctccagc cggagctacg tgactacgtc cacccgcacc tacagcctgg
      181 gcagcgcgct gcgccccagc accagccgca gcctctacgc ctcgtccccg ggcggcgtgt
      241 atgccacgcg ctcctctgcc gtgcgcctgc ggagcagcgt gcccggggtg cggctcctgc
      301 aggactcggt ggacttctcg ctggccgacg ccatcaacac cgagttcaag aacacccgca
      361 ccaacgagaa ggtggagctg caggagctga atgaccgctt cgccaactac atcgacaagg
      421 tgcgcttcct ggagcagcag aataagatcc tgctggccga gctcgagcag ctcaagggcc
      481 aaggcaagtc gcgcctgggg gacctctacg aggaggagat gcgggagctg cgccggcagg
      541 tggaccagct aaccaacgac aaagcccgcg tcgaggtgga gcgcgacaac ctggccgagg
      601 acatcatgcg cctccgggag aaattgcagg aggagatgct tcagagagag gaagccgaaa
      661 acaccctgca atctttcaga caggatgttg acaatgcgtc tctggcacgt cttgaccttg
      721 aacgcaaagt ggaatctttg caagaagaga ttgccttttt gaagaaactc cacgaagagg
      781 aaatccagga gctgcaggct cagattcagg aacagcatgt ccaaatcgat gtggatgttt
      841 ccaagcctga cctcacggct gccctgcgtg acgtacgtca gcaatatgaa agtgtggctg
      901 ccaagaacct gcaggaggca gaagaatggt acaaatccaa gtttgctgac ctctctgagg
      961 ctgccaaccg gaacaatgac gccctgcgcc aggcaaagca ggagtccact gagtaccgga
     1021 gacaggtgca gtccctcacc tgtgaagtgg atgcccttaa aggaaccaat gagtccctgg
     1081 aacgccagat gcgtgaaatg gaagagaact ttgccgttga agctgctaac taccaagaca
     1141 ctattggccg cctgcaggat gagattcaga atatgaagga ggaaatggct cgtcaccttc
     1201 gtgaatacca agacctgctc aatgttaaga tggcccttga cattgagatt gccacctaca
     1261 ggaagctgct ggaaggcgag gagagcagga tttctctgcc tcttccaaac ttttcctccc
     1321 tgaacctgag ggaaactaat ctggattcac tccctctggt tgatacccac tcaaaaagga
     1381 cacttctgat taagacggtt gaaactagag atggacaggt tatcaacgaa acttctcagc
     1441 atcacgatga ccttgaaggg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg
     1501 ataacatggc catcattaag gagttcatgc gcttcaaggt gcacatggag gggtccgtga
     1561 acggccacgt gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
     1621 ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cacctgggac atcctgagcc
     1681 ctcagttcat gtacggctcc aatgcctacg tgaagcaccc cgccgacatc cccgactact
     1741 ttaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaaattc gaggacggcg
     1801 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
     1861 agctgcgcgg caccaacttc ccctccgacg gccccgtgat gcagaagaag accatgggct
     1921 gggaggccct ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gaggtcaagc
     1981 ccagagtgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
     2041 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa ccgcaagctg gacatcacca
     2101 gccacaacga ggactacacc atcgtggagc agtacgagag agccgagggc cgccactcca
     2161 ccggcggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat aatcagccat
     2221 accacatt
//
