LOCUS       sequence-8835-ex       11282 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pPIC3.5K converted Gateway Destionation vector for multiple copy 
            integration of gene of interest into Pichia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11282)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 11282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pEZYpci
FEATURES             Location/Qualifiers
     source          1..11282
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..937
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     protein_bind    959..1083
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1512..1817)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1614..1633)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2162..2821)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2736..2755
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2822..2924)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(3102..3226)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3327..3573
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(3986..6520)
                     /codon_start=1
                     /gene="Pichia pastoris HIS4"
                     /product="multifunctional enzyme, required for histidine 
                     biosynthesis"
                     /label=PpHIS4
                     /note="auxotrophic marker for Pichia pastoris"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE
                     LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL
                     VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII
                     DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI
                     PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER
                     TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED
                     IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP
                     KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE
                     LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP
                     GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE
                     VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA
                     LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED
                     AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV
                     QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK
                     HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
     CDS             complement(6934..7749)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6934..7749)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7657..7676
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7741..7760
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(7914..7933)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    8128..8884
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     primer_bind     complement(8919..8941)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9027..9167
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9082..9101
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9182..9199)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9353..9941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9433..9452)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10112..10903,10904..10972))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10735..10754
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10973..11077)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11145..11163
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaagga tctgcagaat tgattaatac
      961 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     1021 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     1081 atgaatcaac tacttagatg gtattagtga cctgtagtcg accgacagcc ttccaaatgt
     1141 tcttcgggtg atgctgccaa cttagtcgac cgacagcctt ccaaatgttc ttctcaaacg
     1201 gaatcgtcgt atccagccta ctcgctattg tcctcaatgc cgtattaaat cataaaaaga
     1261 aataagaaaa agaggtgcga gcctcttttt tgtgtgacaa aataaaaaca tctacctatt
     1321 catatacgct agtgtcatag tcctgaaaat catctgcatc aagaacaatt tcacaactct
     1381 tatacttttc tcttacaagt cgttcggctt catctggatt ttcagcctct atacttacta
     1441 aacgtgataa agtttctgta atttctactg tatcgacctg cagactggct gtgtataagg
     1501 gagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg
     1561 cggtggctga gatcagccac ttcttccccg ataacggaga ccggcacact ggccatatcg
     1621 gtggtcatca tgcgccagct ttcatccccg atatgcacca ccgggtaaag ttcacgggag
     1681 actttatctg acagcagacg tgcactggcc agggggatca ccatccgtcg cccgggcgtg
     1741 tcaataatat cactctgtac atccacaaac agacgataac ggctctctct tttataggtg
     1801 taaaccttaa actgcatttc accagcccct gttctcgtca gcaaaagagc cgttcatttc
     1861 aataaaccgg gcgacctcag ccatcccttc ctgattttcc gctttccagc gttcggcacg
     1921 cagacgacgg gcttcattct gcatggttgt gcttaccaga ccggagatat tgacatcata
     1981 tatgccttga gcaactgata gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata
     2041 gcatacctct ttttgacata cttcgggtat acatatcagt atatattctt ataccgcaaa
     2101 aatcagcgcg caaatacgca tactgttatc tggcttttag taagccggat ccacgcggcg
     2161 tttacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2221 catggaagcc atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2281 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2341 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2401 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2461 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2521 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2581 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
     2641 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2701 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2761 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2821 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2881 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc
     2941 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga
     3001 agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact
     3061 tagtcgacta caggtcacta ataccatcta agtagttgat tcatagtgac tggatatgtt
     3121 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
     3181 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtcctc gacaagcttg
     3241 cggccgcgaa ttaattcgcc ttagacatga ctgttcctca gttcaagttg ggcacttacg
     3301 agaagaccgg tcttgctaga ttctaatcaa gaggatgtca gaatgccatt tgcctgagag
     3361 atgcaggctt catttttgat acttttttat ttgtaaccta tatagtatag gatttttttt
     3421 gtcattttgt ttcttctcgt acgagcttgc tcctgatcag cctatctcgc agctgatgaa
     3481 tatcttgtgg taggggtttg ggaaaatcat tcgagtttga tgtttttctt ggtatttccc
     3541 actcctcttc agagtacaga agattaagtg agaagttcgt ttgtgcaagc ttatcgataa
     3601 gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa
     3661 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg
     3721 cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg acagcatcgc
     3781 cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat gcgcacccgt
     3841 tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt cgctacttgg
     3901 agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggatct atcgaatcta
     3961 aatgtaagtt aaaatctcta aataattaaa taagtcccag tttctccata cgaaccttaa
     4021 cagcattgcg gtgagcatct agaccttcaa cagcagccag atccatcact gcttggccaa
     4081 tatgtttcag tccctcagga gttacgtctt gtgaagtgat gaacttctgg aaggttgcag
     4141 tgttaactcc gctgtattga cgggcatatc cgtacgttgg caaagtgtgg ttggtaccgg
     4201 aggagtaatc tccacaactc tctggagagt aggcaccaac aaacacagat ccagcgtgtt
     4261 gtacttgatc aacataagaa gaagcattct cgatttgcag gatcaagtgt tcaggagcgt
     4321 actgattgga catttccaaa gcctgctcgt aggttgcaac cgatagggtt gtagagtgtg
     4381 caatacactt gcgtacaatt tcaacccttg gcaactgcac agcttggttg tgaacagcat
     4441 cttcaattct ggcaagctcc ttgtctgtca tatcgacagc caacagaatc acctgggaat
     4501 caataccatg ttcagcttga gacagaaggt ctgaggcaac gaaatctgga tcagcgtatt
     4561 tatcagcaat aactagaact tcagaaggcc cagcaggcat gtcaatacta cacagggctg
     4621 atgtgtcatt ttgaaccatc atcttggcag cagtaacgaa ctggtttcct ggaccaaata
     4681 ttttgtcaca cttaggaaca gtttctgttc cgtaagccat agcagctact gcctgggcgc
     4741 ctcctgctag cacgatacac ttagcaccaa ccttgtgggc aacgtagatg acttctgggg
     4801 taagggtacc atccttctta ggtggagatg caaaaacaat ttctttgcaa ccagcaactt
     4861 tggcaggaac acccagcatc agggaagtgg aaggcagaat tgcggttcca ccaggaatat
     4921 agaggccaac tttctcaata ggtcttgcaa aacgagagca gactacacca gggcaagtct
     4981 caacttgcaa cgtctccgtt agttgagctt catggaattt cctgacgtta tctatagaga
     5041 gatcaatggc tctcttaacg ttatctggca attgcataag ttcctctggg aaaggagctt
     5101 ctaacacagg tgtcttcaaa gcgactccat caaacttggc agttagttct aaaagggctt
     5161 tgtcaccatt ttgacgaaca ttgtcgacaa ttggtttgac taattccata atctgttccg
     5221 ttttctggat aggacgacga agggcatctt caatttcttg tgaggaggcc ttagaaacgt
     5281 caattttgca caattcaata cgaccttcag aagggacttc tttaggtttg gattcttctt
     5341 taggttgttc cttggtgtat cctggcttgg catctccttt ccttctagtg acctttaggg
     5401 acttcatatc caggtttctc tccacctcgt ccaacgtcac accgtacttg gcacatctaa
     5461 ctaatgcaaa ataaaataag tcagcacatt cccaggctat atcttccttg gatttagctt
     5521 ctgcaagttc atcagcttcc tccctaattt tagcgttcaa caaaacttcg tcgtcaaata
     5581 accgtttggt ataagaacct tctggagcat tgctcttacg atcccacaag gtggcttcca
     5641 tggctctaag accctttgat tggccaaaac aggaagtgcg ttccaagtga cagaaaccaa
     5701 cacctgtttg ttcaaccaca aatttcaagc agtctccatc acaatccaat tcgataccca
     5761 gcaacttttg agttgctcca gatgtagcac ctttatacca caaaccgtga cgacgagatt
     5821 ggtagactcc agtttgtgtc cttatagcct ccggaataga ctttttggac gagtacacca
     5881 ggcccaacga gtaattagaa gagtcagcca ccaaagtagt gaatagacca tcggggcggt
     5941 cagtagtcaa agacgccaac aaaatttcac tgacagggaa ctttttgaca tcttcagaaa
     6001 gttcgtattc agtagtcaat tgccgagcat caataatggg gattatacca gaagcaacag
     6061 tggaagtcac atctaccaac tttgcggtct cagaaaaagc ataaacagtt ctactaccgc
     6121 cattagtgaa acttttcaaa tcgcccagtg gagaagaaaa aggcacagcg atactagcat
     6181 tagcgggcaa ggatgcaact ttatcaacca gggtcctata gataacccta gcgcctggga
     6241 tcatcctttg gacaactctt tctgccaaat ctaggtccaa aatcacttca ttgataccat
     6301 tattgtacaa cttgagcaag ttgtcgatca gctcctcaaa ttggtcctct gtaacggatg
     6361 actcaacttg cacattaact tgaagctcag tcgattgagt gaacttgatc aggttgtgca
     6421 gctggtcagc agcataggga aacacggctt ttcctaccaa actcaaggaa ttatcaaact
     6481 ctgcaacact tgcgtatgca ggtagcaagg gaaatgtcat acttgaagtc ggacagtgag
     6541 tgtagtcttg agaaattctg aagccgtatt tttattatca gtgagtcagt catcaggaga
     6601 tcctctacgc cggacgcatc gtggccgacc tgcagggggg gggggggcgc tgaggtctgc
     6661 ctcgtgaaga aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga
     6721 aagtgaggga gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga
     6781 acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca
     6841 actcagcaaa agttcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct
     6901 ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg
     6961 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
     7021 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
     7081 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
     7141 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt
     7201 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
     7261 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
     7321 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
     7381 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
     7441 cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
     7501 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
     7561 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
     7621 caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
     7681 taaatcagca tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat
     7741 atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga
     7801 tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttc
     7861 cccccccccc ctgcaggtcg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     7921 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     7981 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     8041 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     8101 aatgcaggag tcgcataagg gagagcgtcg agtatctatg attggaagta tgggaatggt
     8161 gatacccgca ttcttcagtg tcttgaggtc tcctatcaga ttatgcccaa ctaaagcaac
     8221 cggaggagga gatttcatgg taaatttctc tgacttttgg tcatcagtag actcgaactg
     8281 tgagactatc tcggttatga cagcagaaat gtccttcttg gagacagtaa atgaagtccc
     8341 accaataaag aaatccttgt tatcaggaac aaacttcttg tttcgaactt tttcggtgcc
     8401 ttgaactata aaatgtagag tggatatgtc gggtaggaat ggagcgggca aatgcttacc
     8461 ttctggacct tcaagaggta tgtagggttt gtagatactg atgccaactt cagtgacaac
     8521 gttgctattt cgttcaaacc attccgaatc cagagaaatc aaagttgttt gtctactatt
     8581 gatccaagcc agtgcggtct tgaaactgac aatagtgtgc tcgtgttttg aggtcatctt
     8641 tgtatgaata aatctagtct ttgatctaaa taatcttgac gagccaaggc gataaatacc
     8701 caaatctaaa actcttttaa aacgttaaaa ggacaagtat gtctgcctgt attaaacccc
     8761 aaatcagctc gtagtctgat cctcatcaac ttgaggggca ctatcttgtt ttagagaaat
     8821 ttgcggagat gcgatatcga gaaaaaggta cgctgatttt aaacgtgaaa tttatctcaa
     8881 gatctctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     8941 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     9001 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     9061 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     9121 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
     9181 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9241 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9301 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9361 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9421 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9481 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9541 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9601 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9661 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9721 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9781 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9841 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9901 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9961 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10021 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10081 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10141 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10201 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10261 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10321 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    10381 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg
    10441 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10501 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10561 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    10621 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10681 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
    10741 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10801 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10861 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10921 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10981 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    11041 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    11101 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
    11161 ggccctttcg tcttcaagaa ttaattctca tgtttgacag cttatcatcg ataagctgac
    11221 tcatgttggt attgtgaaat agacgcagat cgggaacact gaaaaataac agttattatt
    11281 cg
//
