LOCUS       sequence-88352-e        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Alpha-V-Integrin-25
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    228..280
                     /label=MCS
                     /note="multiple cloning site"
     CDS             296..1012
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 attggttacc actaatgtca cctggggcat tcagccagcg cccatgcctg tgcctgtgtg
       61 ggtgatcatt ttagcagttc tagcaggatt gttgctactg gctgttttgg tatttgtaat
      121 gtacaggatg ggctttttta aacgggtccg gccacctcaa gaagaacaag aaagggagca
      181 gcttcaacct catgaaaatg gtgaaggaaa ctcagaaact ccgggatctc gagctcaagc
      241 ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc ccaccggtcg ccaccatgag
      301 caagggcgcc gagctgttca ccggcatcgt gcccatcctg atcgagctga atggcgatgt
      361 gaatggccac aagttcagcg tgagcggcga gggcgagggc gatgccacct acggcaagct
      421 gaccctgaag ttcatctgca ccaccggcaa gctgcctgtg ccctggccca ccctggtggc
      481 caccctgagc tacggcgtgc agtgcttctc acgctacccc gatcacatga agcagcacga
      541 cttcttcaag agcgccatgc ctgagggcta catccaggag cgcaccatct tcttcgagga
      601 tgacggcaac tacaagacgc gcgccgaggt gaagttcgag ggcgataccc tggtgagtcg
      661 catcgagctg accggcactg atttcaagga ggatggcaac atcctgggca ataagatgga
      721 gtacaactac aacgccacca atgtgtacat cgtggccgac aaggccagga atggcatcaa
      781 ggtgaacttc aaggtccgcc acaacatcaa ggatggcagc gtgcagctgg ccgaccacta
      841 ccagcagaat acccccatcg gcgatggccc tgtgctgctg cccgataacc actacctgtc
      901 cacccagagc gccctgtcca aggaccccaa cgagaagcgc gatcacatga tctacctcga
      961 gttcgtgacc gccgccgcca tcacccacgg catggatgag ctgtacaagt aaagcggccg
     1021 cgactctaga tcataatcag ccataccaca ttt
//
