LOCUS       sequence-88357-e        1291 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 400 / 490, Emission: 468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1291)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-CENPA-N-22
FEATURES             Location/Qualifiers
     source          1..1291
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    455..507
                     /label=MCS
                     /note="multiple cloning site"
     CDS             521..1237
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 cccatggctt gtgacgtcag atccgctagc caccatgggc ccgcgccgcc ggagccgaaa
       61 gcccgaggcc ccgaggaggc gcagcccgag cccgaccccg acccccggcc cctcccggcg
      121 gggcccctcc ttaggcgctt cctcccatca acacagtcgg cggagacaag gttggctaaa
      181 ggagatccga aagcttcaga agagcacaca cctcttgata aggaagctgc ccttcagccg
      241 cctggcaaga gaaatatgtg ttaaattcac tcgtggtgtg gacttcaatt ggcaagccca
      301 ggccctattg gccctacaag aggcagcaga agcatttcta gttcatctct ttgaggacgc
      361 ctatctcctc accttacatg caggccgagt tactctcttc ccaaaggatg tgcaactggc
      421 ccggaggatc cggggccttg aggagggact cggcctcgag ctcaagcttc gaattctgca
      481 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atgagcaagg gcgccgagct
      541 gttcaccggc atcgtgccca tcctgatcga gctgaatggc gatgtgaatg gccacaagtt
      601 cagcgtgagc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      661 ctgcaccacc ggcaagctgc ctgtgccctg gcccaccctg gtggccaccc tgagctacgg
      721 cgtgcagtgc ttctcacgct accccgatca catgaagcag cacgacttct tcaagagcgc
      781 catgcctgag ggctacatcc aggagcgcac catcttcttc gaggatgacg gcaactacaa
      841 gacgcgcgcc gaggtgaagt tcgagggcga taccctggtg agtcgcatcg agctgaccgg
      901 cactgatttc aaggaggatg gcaacatcct gggcaataag atggagtaca actacaacgc
      961 caccaatgtg tacatcgtgg ccgacaaggc caggaatggc atcaaggtga acttcaaggt
     1021 ccgccacaac atcaaggatg gcagcgtgca gctggccgac cactaccagc agaatacccc
     1081 catcggcgat ggccctgtgc tgctgcccga taaccactac ctgtccaccc agagcgccct
     1141 gtccaaggac cccaacgaga agcgcgatca catgatctac ctcgagttcg tgaccgccgc
     1201 cgccatcacc cacggcatgg atgagctgta caagtaaagc ggccgcgact ctagatcata
     1261 atcagccata ccacattgta gaagaattcc t
//
