LOCUS       sequence-88358-e        1380 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 400 / 490, Emission: 
            468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1380)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1380
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    542..594
                     /label=MCS
                     /note="multiple cloning site"
     CDS             608..1324
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 agcatgctag gtgacgtcag atccgctagc caccatgggc cccaagcggc ggcagctgac
       61 gttccgggag aagtcgcgga tcatccagga ggtggaggag aacccggacc tacgcaaggg
      121 cgagatcgcg cggcgcttca acatcccgcc gtccacgctg agcacgatcc tgaagaacaa
      181 gcgcgccatc ctggcgtcgg agcgcaagta cggggtggcc tccacctgcc gcaagaccaa
      241 caagctgtct ccctacgaca agctcgaggg cttgctcatc gcctggttcc agcagatccg
      301 cgccgccggc ctgccggtca agggcatcat cctcaaggag aaggcgctgc gcatagccga
      361 ggagctgggc atggacgact tcaccgcctc caacggctgg ctggaccgct tccgccggcg
      421 ccacggcgtg gtgtcctgca gcggcgtggc ccgcgcccgc gcgcgaaacg ctgccccccg
      481 caccccggcg gcgcctgcca gtccggccgc ggtgccctcg gagggcagtg gcgggagcac
      541 tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccggt
      601 cgccaccatg agcaagggcg ccgagctgtt caccggcatc gtgcccatcc tgatcgagct
      661 gaatggcgat gtgaatggcc acaagttcag cgtgagcggc gagggcgagg gcgatgccac
      721 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcctg tgccctggcc
      781 caccctggtg gccaccctga gctacggcgt gcagtgcttc tcacgctacc ccgatcacat
      841 gaagcagcac gacttcttca agagcgccat gcctgagggc tacatccagg agcgcaccat
      901 cttcttcgag gatgacggca actacaagac gcgcgccgag gtgaagttcg agggcgatac
      961 cctggtgagt cgcatcgagc tgaccggcac tgatttcaag gaggatggca acatcctggg
     1021 caataagatg gagtacaact acaacgccac caatgtgtac atcgtggccg acaaggccag
     1081 gaatggcatc aaggtgaact tcaaggtccg ccacaacatc aaggatggca gcgtgcagct
     1141 ggccgaccac taccagcaga atacccccat cggcgatggc cctgtgctgc tgcccgataa
     1201 ccactacctg tccacccaga gcgccctgtc caaggacccc aacgagaagc gcgatcacat
     1261 gatctacctc gagttcgtga ccgccgccgc catcacccac ggcatggatg agctgtacaa
     1321 gtaaagcggc cgcgactcta gatcataatc agccatacca cattgtagaa agttttgcct
//
