LOCUS       sequence-88360-e        2433 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 400 / 490, Emission: 
            468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2433)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-C-Src-7
FEATURES             Location/Qualifiers
     source          1..2433
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..98
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             1677..2393
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcatgg
       61 ggagcagcaa gagcaagccc aaggacccca gccagcgccg gcgcagcctg gagccacccg
      121 acagcaccca ccacggggga ttcccagcct cgcagacccc caacaagaca gcagcccccg
      181 acacgcaccg cacccccagc cgctcctttg ggaccgtggc caccgagccc aagctcttcg
      241 ggggcttcaa cacttctgac accgtcacgt cgccgcagcg tgccggggca ctggctggcg
      301 gcgtcaccac tttcgtggct ctctatgact acgagtcccg gactgaaacg gacttgtcct
      361 tcaagaaagg agaacgcctg cagattgtca acaacacgga aggtgactgg tggctggctc
      421 attccctcac tacaggacag acgggctaca tccccagtaa ctatgtcgcg ccctcagact
      481 ccatccaggc tgaagagtgg tactttggga agatcactcg tcgggagtcc gagcggctgc
      541 tgctcaaccc cgaaaacccc cggggaacct tcttggtccg ggagagcgag acgacaaaag
      601 gtgcctattg cctctccgtt tctgactttg acaacgccaa ggggctcaat gtgaagcact
      661 acaagatccg caagctggac agcggcggct tctacatcac ctcacgcaca cagttcagca
      721 gcctgcagca gctggtggcc tactactcca aacatgctga tggcttgtgc caccgcctga
      781 ccaacgtctg ccccacgtcc aagccccaga cccagggact cgccaaggac gcgtgggaaa
      841 tcccccggga gtcgctgcgg ctggaggtga agctggggca gggctgcttt ggagaggtct
      901 ggatggggac ctggaacggc accaccagag tggccataaa gactctgaag cccggcacca
      961 tgtccccgga ggccttcctg caggaagccc aagtgatgaa gaagctccgg catgagaagc
     1021 tggttcagct gtacgcagtg gtgtcggaag agcccatcta catcgtcact gagtacatga
     1081 gcaaggggag cctcctggat ttcctgaagg gagagatggg caagtacctg cggctgccac
     1141 agctcgtcga tatggctgct cagattgcat ccggcatggc ctatgtggag aggatgaact
     1201 acgtgcaccg agacctgcgg gcggccaaca tcctggtggg ggagaacctg gtgtgcaagg
     1261 tggctgactt tgggctggca cgcctcatcg aggacaacga gtacacagca cggcaaggtg
     1321 ccaagttccc catcaagtgg acagcccccg aggcagccct ctatggccgg ttcaccatca
     1381 agtcggatgt ctggtccttc ggcatcctgc tgactgagct gaccaccaag ggccgggtgc
     1441 catacccagg gatggtcaac agggaggtgc tggaccaggt ggagaggggc taccgcatgc
     1501 cctgcccgcc cgagtgcccc gagtcgctgc atgacctcat gtgccagtgc tggcggaagg
     1561 accctgagga gcggcccact tttgagtacc tgcaggcctt cctggaggac tacttcacct
     1621 cgacagagcc ccagtaccag cctggagaga acctacggga tccaccggtc gccaccatga
     1681 gcaagggcgc cgagctgttc accggcatcg tgcccatcct gatcgagctg aatggcgatg
     1741 tgaatggcca caagttcagc gtgagcggcg agggcgaggg cgatgccacc tacggcaagc
     1801 tgaccctgaa gttcatctgc accaccggca agctgcctgt gccctggccc accctggtgg
     1861 ccaccctgag ctacggcgtg cagtgcttct cacgctaccc cgatcacatg aagcagcacg
     1921 acttcttcaa gagcgccatg cctgagggct acatccagga gcgcaccatc ttcttcgagg
     1981 atgacggcaa ctacaagacg cgcgccgagg tgaagttcga gggcgatacc ctggtgagtc
     2041 gcatcgagct gaccggcact gatttcaagg aggatggcaa catcctgggc aataagatgg
     2101 agtacaacta caacgccacc aatgtgtaca tcgtggccga caaggccagg aatggcatca
     2161 aggtgaactt caaggtccgc cacaacatca aggatggcag cgtgcagctg gccgaccact
     2221 accagcagaa tacccccatc ggcgatggcc ctgtgctgct gcccgataac cactacctgt
     2281 ccacccagag cgccctgtcc aaggacccca acgagaagcg cgatcacatg atctacctcg
     2341 agttcgtgac cgccgccgcc atcacccacg gcatggatga gctgtacaag taaagcggcc
     2401 gcgactctag atcataatca gccataccac att
//
