LOCUS       sequence-88364-e        1671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 400 / 490, 
            Emission: 468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1671)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-EB3-7
FEATURES             Location/Qualifiers
     source          1..1671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             914..1630
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 ggactcagat ctcgagctca agcttcgaat tctgcagtcg actgccacca tggccgtcaa
       61 tgtgtactcc acatctgtga ccagtgaaaa tctgagtcgc catgatatgc ttgcatgggt
      121 caacgactcc ctgcacctca actataccaa gatagaacag ctttgttcag gggcagccta
      181 ctgccagttc atggacatgc tcttccccgg ctgtgtgcac ttgaggaaag tgaagttcca
      241 ggccaaacta gagcatgaat acatccacaa cttcaaggtg ctgcaagcag ctttcaagaa
      301 gatgggtgtt gacaaaatca ttcctgtaga gaaattagtg aaaggaaaat tccaagataa
      361 ttttgagttt attcagtggt ttaagaaatt ctttgacgca aactatgatg gaaaggatta
      421 caaccctctg ctggcgcggc agggccagga cgtagcgcca cctcctaacc caggtgatca
      481 gatcttcaac aaatccaaga aactcattgg cacagcagtt ccacagagga cgtcccccac
      541 aggcccaaaa aacatgcaga cctctggccg gctgagcaat gtggcccccc cctgcattct
      601 ccggaagaat cctccatcag cccgaaatgg cggccatgag actgatgccc aaattcttga
      661 actcaaccaa cagctggtgg acttgaagct gacagtggat gggctggaga aggaacgtga
      721 cttctacttc agcaaacttc gtgacatcga gctcatctgc caggagcatg aaagtgaaaa
      781 cagccctgtt atctcaggca tcattggcat cctctatgcc acagaggaag gattcgcacc
      841 ccctgaggac gatgagattg aagagcatca acaagaagac caggacgagt acctggatcc
      901 accggtcgcc accatgagca agggcgccga gctgttcacc ggcatcgtgc ccatcctgat
      961 cgagctgaat ggcgatgtga atggccacaa gttcagcgtg agcggcgagg gcgagggcga
     1021 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcctgtgcc
     1081 ctggcccacc ctggtggcca ccctgagcta cggcgtgcag tgcttctcac gctaccccga
     1141 tcacatgaag cagcacgact tcttcaagag cgccatgcct gagggctaca tccaggagcg
     1201 caccatcttc ttcgaggatg acggcaacta caagacgcgc gccgaggtga agttcgaggg
     1261 cgataccctg gtgagtcgca tcgagctgac cggcactgat ttcaaggagg atggcaacat
     1321 cctgggcaat aagatggagt acaactacaa cgccaccaat gtgtacatcg tggccgacaa
     1381 ggccaggaat ggcatcaagg tgaacttcaa ggtccgccac aacatcaagg atggcagcgt
     1441 gcagctggcc gaccactacc agcagaatac ccccatcggc gatggccctg tgctgctgcc
     1501 cgataaccac tacctgtcca cccagagcgc cctgtccaag gaccccaacg agaagcgcga
     1561 tcacatgatc tacctcgagt tcgtgaccgc cgccgccatc acccacggca tggatgagct
     1621 gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt t
//
