LOCUS       sequence-88367-e        1232 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Adaptor, Excitation: 400 / 490, Emission: 468
            / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1232)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-H2B-6
FEATURES             Location/Qualifiers
     source          1..1232
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..456
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             475..1191
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgccaccat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc
      121 tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc
      181 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
      241 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
      301 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
      361 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
      421 ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtcgc caccatgagc
      481 aagggcgccg agctgttcac cggcatcgtg cccatcctga tcgagctgaa tggcgatgtg
      541 aatggccaca agttcagcgt gagcggcgag ggcgagggcg atgccaccta cggcaagctg
      601 accctgaagt tcatctgcac caccggcaag ctgcctgtgc cctggcccac cctggtggcc
      661 accctgagct acggcgtgca gtgcttctca cgctaccccg atcacatgaa gcagcacgac
      721 ttcttcaaga gcgccatgcc tgagggctac atccaggagc gcaccatctt cttcgaggat
      781 gacggcaact acaagacgcg cgccgaggtg aagttcgagg gcgataccct ggtgagtcgc
      841 atcgagctga ccggcactga tttcaaggag gatggcaaca tcctgggcaa taagatggag
      901 tacaactaca acgccaccaa tgtgtacatc gtggccgaca aggccaggaa tggcatcaag
      961 gtgaacttca aggtccgcca caacatcaag gatggcagcg tgcagctggc cgaccactac
     1021 cagcagaata cccccatcgg cgatggccct gtgctgctgc ccgataacca ctacctgtcc
     1081 acccagagcg ccctgtccaa ggaccccaac gagaagcgcg atcacatgat ctacctcgag
     1141 ttcgtgaccg ccgccgccat cacccacggc atggatgagc tgtacaagta aagcggccgc
     1201 gactctagat cataatcagc cataccacat tt
//
