LOCUS       sequence-88372-e        1688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Capping Protein , Excitation: 400 / 490, 
            Emission: 468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1688)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-MCPA2-N-18
FEATURES             Location/Qualifiers
     source          1..1688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             940..1656
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 agtgacgtca gatccgctag cgccaccatg gcggatctgg aggagcagtt gtccgatgag
       61 gagaaggttc gtatagcagc aaaattcatc attcacgccc ctcctggaga atttaatgag
      121 gtttttaatg atgttcgact attgcttaat aatgataatc ttctcaggga aggagccgca
      181 catgcatttg cacagtataa cctggaccag tttacaccag taaaaattga aggctatgaa
      241 gatcaggttt tgataacaga acatggagac ttgggaaatg gaaaattttt ggatccaaaa
      301 aacagaatct gtttcaaatt tgatcattta agaaaggaag caactgatcc aaggccctac
      361 gaagcagaaa atgccattga atcctggaga acttcagtag aaactgcact gcgagcttat
      421 gtaaaagagc attatccgaa tggggtctgc actgtgtatg gcaaaaaagt agatgggcag
      481 caaaccatta ttgcatgcat agaaagccat cagtttcaag caaaaaactt ttggaatggt
      541 cgttggaggt cagagtggaa gtttacagtc actccttcca ccacacaggt ggttggcatt
      601 ttgaaaattc aggttcatta ttatgaagat ggtaatgttc agctagtgag tcataaagat
      661 atacaagatt ccctcacagt atctaatgag gtgcaaacag caaaagaatt tataaagatt
      721 gtagaagctg cagaaaatga ataccagact gccatcagtg agaattatca gacgatgtcg
      781 gacactacct tcaaagcctt acgtcgacag ttgccagtga cacgtactaa gatcgactgg
      841 aacaagatcc ttagctacaa gattggcaaa gagatgcaga atgcaagtcg cggaggcagt
      901 ggtggggtac cgcgggcccg ggatccaccg gtcgccacca tgagcaaggg cgccgagctg
      961 ttcaccggca tcgtgcccat cctgatcgag ctgaatggcg atgtgaatgg ccacaagttc
     1021 agcgtgagcg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1081 tgcaccaccg gcaagctgcc tgtgccctgg cccaccctgg tggccaccct gagctacggc
     1141 gtgcagtgct tctcacgcta ccccgatcac atgaagcagc acgacttctt caagagcgcc
     1201 atgcctgagg gctacatcca ggagcgcacc atcttcttcg aggatgacgg caactacaag
     1261 acgcgcgccg aggtgaagtt cgagggcgat accctggtga gtcgcatcga gctgaccggc
     1321 actgatttca aggaggatgg caacatcctg ggcaataaga tggagtacaa ctacaacgcc
     1381 accaatgtgt acatcgtggc cgacaaggcc aggaatggca tcaaggtgaa cttcaaggtc
     1441 cgccacaaca tcaaggatgg cagcgtgcag ctggccgacc actaccagca gaataccccc
     1501 atcggcgatg gccctgtgct gctgcccgat aaccactacc tgtccaccca gagcgccctg
     1561 tccaaggacc ccaacgagaa gcgcgatcac atgatctacc tcgagttcgt gaccgccgcc
     1621 gccatcaccc acggcatgga tgagctgtac aagtaaagcg gccgcgactc tagatcataa
     1681 tcagccat
//
