LOCUS       sequence-88377-e        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 400 / 490, Emission: 468 / 
            511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Mito-7
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        39..138
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     89..109
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             167..241
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             275..991
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 aagtctccac cccatgacgt catgggagtt gtttggcacc aaaatcaacg ggactttcca
       61 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      121 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgt ccgtcctgac
      181 gccgctgctg ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc cgcgcgccaa
      241 gatccattcg ttgggggatc caccggtcgc caccatgagc aagggcgccg agctgttcac
      301 cggcatcgtg cccatcctga tcgagctgaa tggcgatgtg aatggccaca agttcagcgt
      361 gagcggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      421 caccggcaag ctgcctgtgc cctggcccac cctggtggcc accctgagct acggcgtgca
      481 gtgcttctca cgctaccccg atcacatgaa gcagcacgac ttcttcaaga gcgccatgcc
      541 tgagggctac atccaggagc gcaccatctt cttcgaggat gacggcaact acaagacgcg
      601 cgccgaggtg aagttcgagg gcgataccct ggtgagtcgc atcgagctga ccggcactga
      661 tttcaaggag gatggcaaca tcctgggcaa taagatggag tacaactaca acgccaccaa
      721 tgtgtacatc gtggccgaca aggccaggaa tggcatcaag gtgaacttca aggtccgcca
      781 caacatcaag gatggcagcg tgcagctggc cgaccactac cagcagaata cccccatcgg
      841 cgatggccct gtgctgctgc ccgataacca ctacctgtcc acccagagcg ccctgtccaa
      901 ggaccccaac gagaagcgcg atcacatgat ctacctcgag ttcgtgaccg ccgccgccat
      961 cacccacggc atggatgagc tgtacaagta aagcggccgc gactctagat cataatcagc
     1021 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     1081 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc tta
//
