LOCUS       sequence-88378-e        6816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 400 / 490, Emission: 468 / 
            511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6816)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-MyosinIIB-C-18
FEATURES             Location/Qualifiers
     source          1..6816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..758
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 cgcagctagt gacgtcagat ccgctagcgc taccggtcgc caccatgagc aagggcgccg
       61 agctgttcac cggcatcgtg cccatcctga tcgagctgaa tggcgatgtg aatggccaca
      121 agttcagcgt gagcggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      181 tcatctgcac caccggcaag ctgcctgtgc cctggcccac cctggtggcc accctgagct
      241 acggcgtgca gtgcttctca cgctaccccg atcacatgaa gcagcacgac ttcttcaaga
      301 gcgccatgcc tgagggctac atccaggagc gcaccatctt cttcgaggat gacggcaact
      361 acaagacgcg cgccgaggtg aagttcgagg gcgataccct ggtgagtcgc atcgagctga
      421 ccggcactga tttcaaggag gatggcaaca tcctgggcaa taagatggag tacaactaca
      481 acgccaccaa tgtgtacatc gtggccgaca aggccaggaa tggcatcaag gtgaacttca
      541 aggtccgcca caacatcaag gatggcagcg tgcagctggc cgaccactac cagcagaata
      601 cccccatcgg cgatggccct gtgctgctgc ccgataacca ctacctgtcc acccagagcg
      661 ccctgtccaa ggaccccaac gagaagcgcg atcacatgat ctacctcgag ttcgtgaccg
      721 ccgccgccat cacccacggc atggatgagc tgtacaagtc cggaggtagc gcatccagtg
      781 ggggtgcgtc aggcagtggc agcggatctg gcgcgcagag aactggactc gaggatccag
      841 agaggtatct ctttgtggac agggctgtca tctacaaccc tgccactcaa gctgattgga
      901 cagctaaaaa gctagtgtgg attccatcag aacgccatgg ttttgaggca gctagtatca
      961 aagaagaacg gggagatgaa gttatggtgg agttggcaga gaatggaaag aaagcaatgg
     1021 tcaacaaaga tgatattcag aagatgaacc cacctaagtt ttccaaggtg gaggatatgg
     1081 cagaattgac atgcttgaat gaagcttccg ttttacataa tctgaaggat cgctactatt
     1141 caggactaat ctatacttat tctggactct tctgtgtagt tataaaccct tacaagaatc
     1201 ttccaattta ctctgagaat attattgaaa tgtacagagg gaagaagcgt catgagatgc
     1261 ctccacacat ctatgctata tctgaatctg cttacagatg catgcttcaa gatcgtgagg
     1321 accagtcaat tctttgcacg ggtgagtcag gtgctgggaa gacagaaaat acaaagaaag
     1381 ttattcagta ccttgcccat gttgcttctt cacataaagg aagaaaggac cataatattc
     1441 ctggggaact tgaacggcag cttttgcaag caaatccaat tctggaatca tttggaaatg
     1501 cgaagactgt gaaaaatgat aactcatctc gttttggcaa atttattcgg atcaactttg
     1561 atgtaactgg ctatatcgtt ggggccaaca ttgaaacata ccttctggaa aagtctcgtg
     1621 ctgttcgtca agcaaaagat gaacgtactt ttcatatctt ttaccagttg ttatctggag
     1681 caggagaaca cctaaagtct gatttgcttc ttgaaggatt taataactac aggtttctct
     1741 ccaatggcta tattcctatt ccgggacagc aagacaaaga taatttccag gagaccatgg
     1801 aagcaatgca cataatgggc ttctcccatg aagagattct gtcaatgctt aaagtagtat
     1861 cttcagtgct acagtttgga aatatttctt tcaaaaagga gagaaatact gatcaagctt
     1921 ccatgccaga aaatacagtt gcgcagaagc tctgccatct tcttgggatg aatgtgatgg
     1981 agtttactcg ggccatcctg actccccgga tcaaggtcgg ccgagactat gtgcaaaaag
     2041 cccagaccaa agaacaggca gattttgcag tagaagcatt ggcaaaagct acctatgagc
     2101 ggctctttcg ctggctcgtt catcgcatca ataaagctct ggataggacc aaacgtcagg
     2161 gagcatcttt cattggaatc ctggatattg ctggatttga aatttttgag ctgaactcct
     2221 ttgaacaact ttgcatcaac tacaccaatg agaagctgca gcagctgttc aaccacacca
     2281 tgtttatcct agaacaagag gaataccagc gcgaaggcat cgagtggaac ttcatcgatt
     2341 tcgggctgga tctgcagcca tgcatcgacc taatagagag acctgcgaac cctcctggtg
     2401 tactggccct tttggatgaa gaatgctggt tccctaaagc cacagataaa acctttgttg
     2461 aaaaactggt tcaagagcaa ggttcccact ccaagtttca gaaacctcga caattaaaag
     2521 acaaagctga tttttgcatt atacattatg cagggaaggt ggactataag gcagatgagt
     2581 ggctgatgaa gaatatggac cccctgaatg acaacgtggc cacccttttg caccagtcat
     2641 cagacagatt tgtggcagag ctttggaaag atgtggaccg tatcgtgggt ctggatcaag
     2701 tcactggtat gactgagaca gcttttggct ccgcatataa aaccaagaag ggcatgtttc
     2761 gtaccgttgg gcaactctac aaagaatctc tcaccaagct gatggcaact ctccgaaaca
     2821 ccaaccctaa ctttgttcgt tgtatcattc caaatcacga gaagagggct ggaaaattgg
     2881 atccacacct agtcctagat cagcttcgct gtaatggtgt cctggaaggg atccgaatct
     2941 gtcgccaggg cttccctaac cgaatagttt tccaggaatt cagacagaga tatgagatcc
     3001 taactccaaa tgctattcct aaaggtttta tggatggtaa acaggcctgt gaacgaatga
     3061 tccgggcttt agaattggac ccaaacttgt acagaattgg acagagcaag atatttttca
     3121 gagctggagt tctggcacac ttagaggaag aaagagattt aaaaatcacc gatatcatta
     3181 tcttcttcca ggccgtttgc agaggttacc tggccagaaa ggcctttgcc aagaagcagc
     3241 agcaactaag tgccttaaag gtcttgcagc ggaactgtgc cgcgtacctg aaattacggc
     3301 actggcagtg gtggcgagtc ttcacaaagg tgaagccgct tctacaagtg actcgccagg
     3361 aggaagaact tcaggccaaa gatgaagagc tgttgaaggt gaaggagaag cagacaaagg
     3421 tggaaggaga gctggaggag atggagcgga agcaccagca gcttttagaa gagaagaata
     3481 tccttgcaga acaactacaa gcagagactg agctctttgc tgaagcagaa gagatgaggg
     3541 caagacttgc tgctaaaaag caggaattag aagagattct acatgacttg gagtctaggg
     3601 ttgaagaaga agaagaaaga aaccaaatcc tccaaaatga aaagaaaaaa atgcaagcac
     3661 atattcagga cctggaagaa cagctagacg aggaggaagg ggctcggcaa aagctgcagc
     3721 tggaaaaggt gacagcagag gccaagatca agaagatgga agaggagatt ctgcttctcg
     3781 aggaccaaaa ttccaagttc atcaaagaaa agaaactcat ggaagatcgc attgctgagt
     3841 gttcctctca gctggctgaa gaggaagaaa aggcgaaaaa cttggccaaa atcaggaata
     3901 agcaagaagt gatgatctca gatctagaag aacgcttaaa gaaggaagaa aagactcgtc
     3961 aggaactgga aaaggccaaa agaaaactcg acggggagac gaccgacctg caggaccaga
     4021 tcgcagagct gcaggcgcag attgatgagc tcaagctgca gctggccaag aaggaggagg
     4081 agctgcaggg cgcactggcc agaggtgatg atgaaacact ccataagaac aatgccctta
     4141 aagttgtgcg agagctacaa gcccaaattg ctgaacttca ggaagacttt gaatccgaga
     4201 aggcttcacg gaacaaggcc gaaaagcaga aaagggactt gagtgaggaa ctggaagctc
     4261 tgaaaacaga gctggaggac acgctggaca ccacggcagc ccagcaggaa ctacgtacaa
     4321 aacgtgaaca agaagtggca gagctgaaga aagctcttga ggaggaaact aagaaccatg
     4381 aagctcaaat ccaggacatg agacaaagac acgcaacagc cctggaggag ctctcagagc
     4441 agctggaaca ggccaagcgg ttcaaagcaa atctagagaa gaacaagcag ggcctggaga
     4501 cagataacaa ggagctggcg tgtgaggtga aggtcctgca gcaggtcaag gctgagtctg
     4561 agcacaagag gaagaagctc gacgcgcagg tccaggagct ccatgccaag gtctctgaag
     4621 gcgacaggct cagggtggag ctggcggaga aagcaagtaa gctgcagaat gagctagata
     4681 atgtctccac ccttctggaa gaagcagaga agaagggtat taaatttgct aaggatgcag
     4741 ctagtcttga gtctcaacta caggatacac aggagcttct tcaggaggag acacgccaga
     4801 aactaaacct gagcagtcgg atccggcagc tggaagagga gaagaacagt cttcaggagc
     4861 agcaggagga ggaggaggag gccaggaaga acctggagaa gcaagtgctg gccctgcagt
     4921 cccagttggc tgataccaag aagaaagtag atgacgacct gggaacaatt gaaagtctgg
     4981 aagaagccaa gaagaagctt ctgaaggacg cggaggccct gagccagcgc ctggaggaga
     5041 aggcactggc gtatgacaaa ctggagaaga ccaagaaccg cctgcagcag gagctggacg
     5101 acctcacggt ggacctggac caccagcgcc aggtcgcctc caacttggag aagaagcaga
     5161 agaagtttga ccagctgtta gcagaagaga agagcatctc tgctcgctat gccgaagagc
     5221 gggaccgggc cgaagccgag gccagagaga aagaaaccaa agccctgtca ctggcccggg
     5281 ccctcgagga agccctggag gccaaggagg agtttgagag gcagaacaag cagctccgag
     5341 cagacatgga agacctcatg agctccaaag atgatgtggg aaaaaacgtt cacgaacttg
     5401 aaaaatccaa acgggcccta gagcagcagg tggaggaaat gaggacccag ctggaggagc
     5461 tggaagacga actccaggcc acggaagatg ccaagcttcg tctggaggtc aacatgcagg
     5521 ccatgaaggc gcagttcgag agagacctgc aaaccaggga tgagcagaat gaagagaaga
     5581 agcggctgct gatcaaacag gtgcgggagc tcgaggcgga gctggaggat gagaggaaac
     5641 agcgggcgct tgctgtagct tcaaagaaaa agatggagat agacctgaag gacctcgaag
     5701 cccaaatcga ggctgcgaac aaagctcggg atgaggtgat taagcagctc cgcaagctcc
     5761 aggctcagat gaaggattac caacgtgaat tagaagaagc tcgtgcatcc agagatgaga
     5821 tttttgctca atccaaagag agtgaaaaga aattgaagag tctggaagca gaaatccttc
     5881 aattgcagga ggaacttgcc tcatctgagc gagcccgccg acacgccgag caggagagag
     5941 atgagctggc ggacgagatc accaacagcg cctctggcaa gtccgcgctg ctggatgaga
     6001 agcggcgtct ggaagctcgg atcgcacagc tggaggagga gctggaagag gagcagagca
     6061 acatggagct gctcaacgac cgcttccgca agaccactct acaggtggac acactgaacg
     6121 ccgagctagc agccgagcgc agcgccgccc agaagagtga caatgcacgc cagcaactgg
     6181 agcggcagaa caaggagctg aaggccaagc tgcaggaact cgagggtgct gtcaagtcta
     6241 agttcaaggc caccatctca gccctggagg ccaagattgg gcagctggag gagcagcttg
     6301 agcaggaagc caaggaacga gcagccgcca acaaattagt ccgtcgcact gagaagaagc
     6361 tgaaagaaat cttcatgcag gttgaggatg agcgtcgaca cgcggaccag tataaagagc
     6421 agatggagaa ggccaacgct cggatgaagc agcttaaacg ccagctggag gaagcagaag
     6481 aagaagcgac gcgtgccaac gcatctcggc gtaaactcca gcgggaactg gatgatgcca
     6541 ccgaggccaa cgagggcctg agccgcgagg tcagcaccct gaagaaccgg ctgaggcggg
     6601 gtggccccat cagcttctct tccagccgat ctggccggcg ccagctgcac cttgaaggag
     6661 cttccctgga gctctccgac gatgacacag aaagtaagac cagtgatgtc aacgagacgc
     6721 agccacccca gtcagagtaa ggtaccgcgg gcccgggatc caccggatct agataactga
     6781 tcataatcag ccataccaca ttgtagaaaa tagcat
//
