LOCUS       sequence-88381-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 400 / 490, Emission: 468 
            / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    189..241
                     /label=MCS
                     /note="multiple cloning site"
     CDS             255..971
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 agcgccgcgg ctcgctgtgc tctggctgcc agaagcccat cacgggacgc tgcatcactg
       61 ccatgggcaa gaaatttcac cccgaacact ttgtctgtgc cttctgcctc aagcagctca
      121 acaaaggaac cttcaaagaa cagaacgaca agccctactg tcagaactgc tttctcaaac
      181 tcttctgtct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
      241 caccggtcgc caccatgagc aagggcgccg agctgttcac cggcatcgtg cccatcctga
      301 tcgagctgaa tggcgatgtg aatggccaca agttcagcgt gagcggcgag ggcgagggcg
      361 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcctgtgc
      421 cctggcccac cctggtggcc accctgagct acggcgtgca gtgcttctca cgctaccccg
      481 atcacatgaa gcagcacgac ttcttcaaga gcgccatgcc tgagggctac atccaggagc
      541 gcaccatctt cttcgaggat gacggcaact acaagacgcg cgccgaggtg aagttcgagg
      601 gcgataccct ggtgagtcgc atcgagctga ccggcactga tttcaaggag gatggcaaca
      661 tcctgggcaa taagatggag tacaactaca acgccaccaa tgtgtacatc gtggccgaca
      721 aggccaggaa tggcatcaag gtgaacttca aggtccgcca caacatcaag gatggcagcg
      781 tgcagctggc cgaccactac cagcagaata cccccatcgg cgatggccct gtgctgctgc
      841 ccgataacca ctacctgtcc acccagagcg ccctgtccaa ggaccccaac gagaagcgcg
      901 atcacatgat ctacctcgag ttcgtgaccg ccgccgccat cacccacggc atggatgagc
      961 tgtacaagta aagcggccgc gactctaga
//
