LOCUS       sequence-88382-e        1714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 400 / 490, Emission: 
            468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1714)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..1714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..50
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             54..74
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             78..98
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             111..824
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 gaccgtcaga tccgctagcc accatggatc caaaaaagaa gagaaaggta gatccaaaaa
       61 agaagagaaa ggtagatcca aaaaagaaga gaaaggtacc ggtcgccacc atgagcaagg
      121 gcgccgagct gttcaccggc atcgtgccca tcctgatcga gctgaatggc gatgtgaatg
      181 gccacaagtt cagcgtgagc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      241 tgaagttcat ctgcaccacc ggcaagctgc ctgtgccctg gcccaccctg gtggccaccc
      301 tgagctacgg cgtgcagtgc ttctcacgct accccgatca catgaagcag cacgacttct
      361 tcaagagcgc catgcctgag ggctacatcc aggagcgcac catcttcttc gaggatgacg
      421 gcaactacaa gacgcgcgcc gaggtgaagt tcgagggcga taccctggtg agtcgcatcg
      481 agctgaccgg cactgatttc aaggaggatg gcaacatcct gggcaataag atggagtaca
      541 actacaacgc caccaatgtg tacatcgtgg ccgacaaggc caggaatggc atcaaggtga
      601 acttcaaggt ccgccacaac atcaaggatg gcagcgtgca gctggccgac cactaccagc
      661 agaatacccc catcggcgat ggccctgtgc tgctgcccga taaccactac ctgtccaccc
      721 agagcgccct gtccaaggac cccaacgaga agcgcgatca catgatctac ctcgagttcg
      781 tgaccgccgc cgccatcacc cacggcatgg atgagctgta caagtccgga gaagggcaag
      841 ggcaagggca agggccgggc cgcggctacg cgtatcgatc catgttcgag gcgcgcctgg
      901 tccagggctc catcctcaag aaggtgttgg aggcactcaa ggacctcatc aacgaggcct
      961 gctgggatat tagctccagc ggtgtaaacc tgcagagcat ggactcgtcc cacgtctctt
     1021 tggtgcagct caccctgcgg tctgagggct tcgacaccta ccgctgcgac cgcaacctgg
     1081 ccatgggcgt gaacctcacc agtatgtcca aaatactaaa atgcgccggc aatgaagata
     1141 tcattacact aagggccgaa gataacgcgg ataccttggc gctagtattt gaagcaccaa
     1201 accaggagaa agtttcagac tatgaaatga agttgatgga tttagatgtt gaacaacttg
     1261 gaattccaga acaggagtac agctgtgtag taaagatgcc ttctggtgaa tttgcacgta
     1321 tatgccgaga tctcagccat attggagatg ctgttgtaat ttcctgtgca aaagacggag
     1381 tgaaattttc tgcaagtgga gaacttggaa atggaaacat taaattgtca cagacaagta
     1441 atgtcgataa agaggaggaa gctgttacca tagagatgaa tgaaccagtt caactaactt
     1501 ttgcactgag gtacctgaac ttctttacaa aagccactcc actctcttca acggtgacac
     1561 tcagtatgtc tgcagatgta ccccttgttg tagagtataa aattgcggat atgggacact
     1621 taaaatacta cttggctccc aagatcgagg atgaagaagg atcttaggga tccaccggat
     1681 ctagataact gatcataatc agccatacca catt
//
