LOCUS       sequence-88383-e        3040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 400 / 490, Emission: 468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3040)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-PSD95-N-14
FEATURES             Location/Qualifiers
     source          1..3040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      51..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2271..2987
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 gcatgctagt gacgtcagat ccgctagcgc taccggactc agatctcgag gccaccatgg
       61 actgtctctg tatagtgaca accaagaaat accgctacca agatgaagac acgccccctc
      121 tggaacacag cccggcccac ctccccaacc aggccaattc tccccctgtg attgtcaaca
      181 cggacaccct agaagcccca ggatatgagt tgcaggtgaa tggaacagag ggggagatgg
      241 agtatgagga gatcacattg gaaaggggta actcaggtct gggcttcagc atcgcaggtg
      301 gcactgacaa cccgcacatc ggtgacgacc cgtccatttt tatcaccaag atcattcctg
      361 gtggggctgc agcccaggat ggccgcctca gggtcaatga cagcatcctg tttgtaaatg
      421 aagtggatgt tcgggaggtg acccattcag ctgcggtgga ggccctcaaa gaggcaggtt
      481 ccatcgttcg cctctatgtc atgcgccgga aacccccagc cgaaaaggtc atggagatca
      541 aactcatcaa agggcctaaa ggacttggct tcagcattgc ggggggcgtt gggaaccagc
      601 acatccctgg agataacagc atctatgtaa cgaagatcat cgaaggaggt gctgcccaca
      661 aggatggcag gttgcagatt ggagacaaga tcctggcggt caacagtgtg gggctggagg
      721 acgtcatgca cgaggatgcc gtggcagccc tgaagaacac atatgacgtt gtgtacctaa
      781 aggtggccaa gcccagcaat gcctacctga gtgacagcta tgctccccca gacatcacaa
      841 cctcgtattc tcagcacctg gacaatgaga tcagtcatag cagctacttg ggcactgact
      901 accccacagc catgaccccc acttcccctc ggcgctactc ccctgtggcc aaggacctgc
      961 tgggggagga agacattccc cgggaaccaa ggcggatcgt gatccatcgg ggctccaccg
     1021 gcctgggctt caacatcgtg ggcggcgagg atggtgaagg catcttcatc tccttcatcc
     1081 ttgctggggg tccagccgac ctcagtgggg agctacggaa gggggaccag atcctgtcgg
     1141 tcaatggtgt tgacctccgc aatgccagtc acgaacaggc tgccattgcc ctgaagaatg
     1201 cgggtcagac ggtcacgatc atcgctcagt ataaaccaga agagtatagt cgattcgagg
     1261 ccaagatcca tgatcttcgg gaacagctca tgaatagtag cctaggctca gggactgcat
     1321 ccttgcgaag caaccccaag aggggcttct acattagggc cctgtttgat tacgacaaga
     1381 ccaaggactg cggtttcttg agccaggccc tgagcttccg cttcggggat gtgcttcatg
     1441 tcattgacgc tggtgacgaa gagtggtggc aagcacggcg ggtccactcc gacagtgaga
     1501 ccgacgacat tggcttcatt cccagcaaac ggcgggtcga gcgacgagag tggtcaaggt
     1561 taaaggccaa ggactggggc tccagctctg gatcacaggg tcgagaagac tcggttctga
     1621 gctatgagac ggtgacccag atggaagtgc actatgctcg tcccatcatc atccttggac
     1681 ccaccaaaga ccgtgccaac gatgatcttc tctccgagtt ccccgacaag tttggatcct
     1741 gtgtccctca tacgacacgt cctaagcggg aatatgagat agacggccgg gattaccact
     1801 ttgtctcctc ccgggagaaa atggagaagg acatccaggc acacaagttc attgaggctg
     1861 gccagtacaa cagccacctc tatgggacca gcgtccagtc tgtgcgagag gtagcagagc
     1921 aggggaagca ctgcatcctc gatgtctcgg ccaatgccgt gcggcggctg caggcggccc
     1981 acctgcaccc catcgccatc ttcatccgtc cccgctccct ggagaatgtg ctagagatca
     2041 ataagcggat cacagaggag caagcccgga aagccttcga cagagccacg aagctggagc
     2101 aggagttcac agagtgcttc tcagccatcg tagagggcga cagctttgaa gagatctatc
     2161 acaaagtgaa acgtgtcatt gaagacctct caggccccta catctgggtc ccagcccgag
     2221 agagactcgg cagcgcatcc ggcggaagcg gaagcggacc ggtcgccacc atgagcaagg
     2281 gcgccgagct gttcaccggc atcgtgccca tcctgatcga gctgaatggc gatgtgaatg
     2341 gccacaagtt cagcgtgagc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     2401 tgaagttcat ctgcaccacc ggcaagctgc ctgtgccctg gcccaccctg gtggccaccc
     2461 tgagctacgg cgtgcagtgc ttctcacgct accccgatca catgaagcag cacgacttct
     2521 tcaagagcgc catgcctgag ggctacatcc aggagcgcac catcttcttc gaggatgacg
     2581 gcaactacaa gacgcgcgcc gaggtgaagt tcgagggcga taccctggtg agtcgcatcg
     2641 agctgaccgg cactgatttc aaggaggatg gcaacatcct gggcaataag atggagtaca
     2701 actacaacgc caccaatgtg tacatcgtgg ccgacaaggc caggaatggc atcaaggtga
     2761 acttcaaggt ccgccacaac atcaaggatg gcagcgtgca gctggccgac cactaccagc
     2821 agaatacccc catcggcgat ggccctgtgc tgctgcccga taaccactac ctgtccaccc
     2881 agagcgccct gtccaaggac cccaacgaga agcgcgatca catgatctac ctcgagttcg
     2941 tgaccgccgc cgccatcacc cacggcatgg atgagctgta caagtaaagc ggccgcgact
     3001 ctagatcata atcagccata ccacattgta gagattgcct
//
