LOCUS       sequence-88384-e        8926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 400 / 490, Emission: 468 
            / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8926)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 8926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Talin-18
FEATURES             Location/Qualifiers
     source          1..8926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..749
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     regulatory      798..807
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccaccatgag caagggcgcc gagctgttca
       61 ccggcatcgt gcccatcctg atcgagctga atggcgatgt gaatggccac aagttcagcg
      121 tgagcggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      181 ccaccggcaa gctgcctgtg ccctggccca ccctggtggc caccctgagc tacggcgtgc
      241 agtgcttctc acgctacccc gatcacatga agcagcacga cttcttcaag agcgccatgc
      301 ctgagggcta catccaggag cgcaccatct tcttcgagga tgacggcaac tacaagacgc
      361 gcgccgaggt gaagttcgag ggcgataccc tggtgagtcg catcgagctg accggcactg
      421 atttcaagga ggatggcaac atcctgggca ataagatgga gtacaactac aacgccacca
      481 atgtgtacat cgtggccgac aaggccagga atggcatcaa ggtgaacttc aaggtccgcc
      541 acaacatcaa ggatggcagc gtgcagctgg ccgaccacta ccagcagaat acccccatcg
      601 gcgatggccc tgtgctgctg cccgataacc actacctgtc cacccagagc gccctgtcca
      661 aggaccccaa cgagaagcgc gatcacatga tctacctcga gttcgtgacc gccgccgcca
      721 tcacccacgg catggatgag ctgtacaagt ccggactcag atctcgagct caagctagct
      781 tcgaattcgc agaagctgcc accatggttg cgctttcgct gaagattagc attgggaatg
      841 tggtgaagac gatgcaattt gagccatcta ccatggtgta cgacgcctgc cgcatgattc
      901 gtgagcggat cccagaggcc ctggctggcc ctcccaacga ctttgggctc tttctgtcag
      961 atgatgaccc caaaaaaggc atctggctag aggctgggaa agctttggac tactacatgc
     1021 tccgaaatgg ggacaccatg gagtacagaa aaaagcagag acccctgaag atacggatgt
     1081 tagacggaac ggtgaagact atcatggtgg atgactccaa gaccgtcaca gacatgctca
     1141 tgaccatttg tgcccgaatt ggtatcacca accatgatga gtattcactg gttcgagagc
     1201 tgatggaaga aaagaaagat gaggggacag ggaccctgag aaaagacaag accctactgc
     1261 gagatgaaaa gaagatggag aagctaaagc agaagttgca cacagacgat gagttgaact
     1321 ggctggacca tgggcggaca ctgagggaac agggagtgga agagcatgag acgttgctgc
     1381 tgcggagaaa gttcttctac tcagaccaga atgtggattc ccgagaccct gtacagctga
     1441 accttctcta tgtgcaggca cgagatgaca tcctgaatgg ctcccatcct gtctcctttg
     1501 acaaggcctg tgaatttgca ggcttccagt gccagatcca gtttggacct cacaatgaac
     1561 agaagcacaa ggctggcttc cttgacctga aggacttcct gcccaaggag tatgtgaagc
     1621 agaagggaga gcgtaagatc tttcaggcac acaagaattg tgggcagatg agtgaaattg
     1681 aggccaaggt acgctacgtg aagctggccc gttccctcaa gacttacggt gtctccttct
     1741 tcctagtcaa ggaaaagatg aaggggaaga ataaactggt gcccaggctg ttgggcatca
     1801 ctaaggagtg tgtgatgcgt gtggatgaga agaccaagga ggtgattcag gaatggagcc
     1861 tcaccaacat caaacgctgg gctgcctctc ccaagagctt tactctggac tttggagact
     1921 atcaggatgg ctactactca gtacagacga cagaaggcga gcagatcgca cagctcattg
     1981 ctggctacat agatatcatc cttaagaaga aaaaaagcaa ggaccatttt gggctggagg
     2041 gagatgaaga gtctactatg ttggaggact cagtgtctcc caaaaagtca acagtccttc
     2101 agcagcagta caaccgagtg gggaaagtgg agcacggctc tgtggctctg ccagccatca
     2161 tgcgctctgg agcctctggt cctgagaatt tccaggtggg gagcatgcca cctgcccagc
     2221 agcagatcac cagtggccag atgcaccgag gacacatgcc acctctgact tcagcccagc
     2281 aggcgctcac tggaaccatt aactccagca tgcaggctgt gcaggctgcc caggccactc
     2341 tggatgactt tgagactcta ccccctcttg gccaggatgc tgcctccaaa gcttggcgta
     2401 agaacaagat ggatgaatca aagcatgaga tccactccca ggtagatgcc atcacagctg
     2461 gcactgcctc tgtggtaaac ctgacagcag gagatcctgc agagacagac tatacagcag
     2521 tgggctgtgc agtcaccacc atctcttcca acctgacgga gatgtcccgt ggggtgaagc
     2581 tattggctgc cctgctggag gatgaaggcg gcaatggccg gccccttctg caagcagcaa
     2641 agggccttgc aggggctgtg tcggaactgc ttcgcagtgc tcagcccgcc agcgctgagc
     2701 ctcgtcagaa cctgctgcag gcagccggga acgtgggcca ggccagtggg gagctgttgc
     2761 agcaaattgg ggaaagtgac actgaccccc acttccagga tgttctaatg cagctagcaa
     2821 acgcagtggc aagtgctgca gccgccctgg tcctcaaggc caagagtgtg gcccagcgca
     2881 cagaggattc cgggcttcag acccaagtga ttgctgcagc tacgcaatgt gctctgtcca
     2941 cttcccagct ggtggcctgt accaaggtgg tagcacctac aatcagctca cctgtgtgcc
     3001 aagaacagct agtcgaggct gggcgactgg tggccaaagc tgtggagggc tgtgtgtctg
     3061 cctcgcaagc agctacagaa gatggacagc tgcttcgagg ggtaggagca gcagccacgg
     3121 ctgtcaccca ggccctcaat gagctgctgc agcacgtgaa ggcccacgcc accggagctg
     3181 ggcctgccgg tcggtatgac caagccactg ataccatcct caccgtcact gaaaacatct
     3241 tcagctccat gggtgatgct ggggagatgg tgcgccaggc ccgcatcctg gcccaagcca
     3301 cctcagacct ggtcaatgct atcaaggccg atgctgaggg ggagagtgat ctggagaact
     3361 ctagaaagct cctgagtgct gccaagatcc tcgctgatgc caccgccaag atggtggagg
     3421 cggccaaggg agcagccgcc caccctgaca gtgaggaaca gcagcagcga ctgcgtgaag
     3481 cagctgaggg gcttcgcatg gccaccaatg cagctgcgca gaacgccatc aagaagaagt
     3541 tggtgcagcg cctggagcat gcagccaagc aagctgcagc ctctgcaaca cagaccattg
     3601 ctgcagccca acatgcagcc tctgccccca aggcctctgc gggcccccag cccctactgg
     3661 tgcagagctg taaggccgtg gcagagcaga ttccgttgct ggtgcagggt gtccgaggaa
     3721 gtcaagctca gcctgacagc cctagtgctc agctcgccct cattgctgcc agccagagct
     3781 tcctgcagcc aggtgggaag atggtggcag cggcaaaagc ctcagtgcca acaattcagg
     3841 accaggcttc agcgatgcag ctgagtcagt gcgcaaagaa cctaggcact gccctggccg
     3901 agctgcgcac tgctgctcag aaggctcagg aggcatgtgg acctttggag atggattctg
     3961 cactgagtgt tgtacaaaat ctagagaaag acctacagga aataaaagca gcagctcgag
     4021 atggcaagct taagccctta cctggggaaa caatggagaa gtgtacccaa gatcttggca
     4081 acagcaccaa agcggtgagc tctgccatcg ccaagctgct gggggagatt gcccagggca
     4141 acgagaacta tgcaggtatt gcagctcggg atgtagctgg tggactaagg tcactagccc
     4201 aggctgcgcg gggtgtggct gcgctgacat cagatcctgc agtgcaggcc attgtgcttg
     4261 acacagccag cgacgttctg gacaaggcca gcagcctcat tgaagaggca aaaaaagcat
     4321 ctggacaccc aggggaccca gaaagccagc aaaggcttgc tcaggtagct aaagcagtga
     4381 cccaagccct gaaccgctgt gtcagctgcc tgcctggcca aagagacgtg gataatgccc
     4441 tacgagccgt tggagatgcc agcaagcgac tcctgagtga cttgcttcct ccaagcacgg
     4501 ggacatttca agaagcacag agccgattga atgaagctgc agctgggtta aatcaggccg
     4561 ccacagaact ggtgcaagcc tcccgaggaa ctcctcagga cctggctcgg gcctccggcc
     4621 gatttggaca ggacttcagc accttcctgg aagctggtgt ggagatggcc ggacaggccc
     4681 cgagccagga ggaccgagcc caggtggtgt ccaacctgaa gggcatatcc atgtcttcaa
     4741 gcaaacttct ccttgccgct aaggccttgt ccacagaccc tgcttctccc aacctcaaga
     4801 gtcagctggc tgcggctgcc cgggcagtga cggacagcat caaccagctc atcaccatgt
     4861 gcacccagca ggcacctggc cagaaggagt gtgacaatgc acttcggcag ctagagacag
     4921 tccgagaact cctggagaat ccagtccagc ccatcaacga catgtcctac ttcggttgct
     4981 tggacagtgt catggagaac tccaaggtcc taggtgaggc catgactggc atctcccaaa
     5041 atgccaagaa tggaaatctg ccggagtttg gagatgccat tgccacagcc tccaaggctc
     5101 tctgtggctt cacggaggca gccgcacagg cagcatatct agttggtgtc tctgacccca
     5161 acagccaagc tggacagcaa ggactggtgg aacccacaca gtttgcccgt gcaaaccagg
     5221 caattcagat ggcctgtcag agtctggggg agcctggctg tacccaggcc caggtgttat
     5281 ctgcagccac tattgtagcc aaacacacat ctgcattgtg taacagctgt cgcctggctt
     5341 ccgctaggac tgccaatccc actgccaagc gccagtttgt acagtcagcc aaggaggtgg
     5401 ccaacagtac agccaatctt gtcaagacca tcaaggcact agatggggac ttcacagaag
     5461 agaaccgtgc ccagtgccga gcagccacag cccctctgct ggaagctgtg gataacctga
     5521 gtgcctttgc ctccaaccct gagttctcca gcgtccctgc ccagatcagc cctgagggca
     5581 gagcagccat ggagcccatt gtaatctctg ctaagacaat gttggagagt gctggagggc
     5641 tcatccagac agctcgggcg ttagcagtca atccccgaga ccccccacgt tggtctgtgc
     5701 tagctggcca ctctcggact gtctcagatt ccatcaagaa acttattaca agcatgaggg
     5761 acaaagcccc agggcagctg gagtgtgaga cagccattgc ggctctgaac agctgcttgc
     5821 gggacctaga tcaggcttcg cttgccgctg tcagccagca gcttgctccc cgtgaaggaa
     5881 tctctcaaga ggctttgcac acccagatgc tcactgcagt gcaagaaatt tctcatctca
     5941 ttgagccgct ggccagcgct gctcgggctg aagcctccca gctgggacac aaggtgtccc
     6001 aaatggccca gtactttgaa ccactcaccc tggctgcagt gggtgctgcg tctaagaccc
     6061 tgagccaccc gcagcagatg gcacttctgg accagactaa aacgttggca gagtctgcct
     6121 tgcagttgct atatactgcc aaggaggctg gtggtaaccc caagcaagca gcacacaccc
     6181 aggaagccct ggaggaggct gtgcagatga tgacagaggc cgtagaagac ctgacgacga
     6241 ccctcaacga agcagccagt gctgcaggag tcgttggcgg catggtggac tctatcactc
     6301 aggccatcaa ccagctagat gaaggaccta tgggtgaccc agaaggctca ttcgtagatt
     6361 accagacaac catggtgagg acagccaagg ccattgctgt cactgttcag gagatggtaa
     6421 ccaagtcaaa caccagccct gaagagctag gccctcttgc caaccagctg accagtgact
     6481 atggccgact ggcctcacaa gccaagcctg cagctgtggc tgctgaaaat gaagagatag
     6541 gcgctcacat caaacaccga gtacaggagc tgggccatgg ctgctctgct ctggtcacca
     6601 aggcaggtgc cttgcagtgt agccccagtg atgtctacac caagaaggag ctcatagagt
     6661 gtgcccgcag agtgtcagag aaggtctccc atgttctggc tgcactccag gctgggaatc
     6721 gtggtaccca ggcctgcatt acagcagcca gtgctgtgtc tggtatcatt gctgacctcg
     6781 acaccaccat catgttcgct actgctggca cacttaaccg tgagggtgct gaaacttttg
     6841 ctgaccaccg ggagggtatc ttaaagacag caaaggttct tgtggaggac accaaggtcc
     6901 tagtgcagaa tgcagctggg agccaggaga agttggcaca agccgcccag tcctccgtgg
     6961 ccaccattac ccgcctcgct gatgtggtca agctcggtgc agccagccta ggagccgaag
     7021 accctgaaac tcaggtggtg ctgatcaatg cagtaaagga cgtagccaag gccctgggtg
     7081 acctcatcag cgctacgaag gctgcagcgg gcaaagttgg ggatgaccct gcagtgtggc
     7141 agctcaagaa ctctgccaag gtgatggtga ccaatgtgac atcattgctc aagacagtga
     7201 aggctgtgga agatgaggcc accaaaggca cacgggccct agaggcaacc acagagcaca
     7261 tacgtcagga actggcggtc ttctgttccc cagagccacc tgccaagacc tctacccctg
     7321 aagacttcat ccgaatgacc aagggtatta ctatggcaac agccaaagcc gttgctgctg
     7381 gcaattcctg tcgacaggaa gatgtcattg ccacagccaa tctgagccga cgggctattg
     7441 cggacatgct tcgggcttgc aaggaagcag ctttccaccc agaagtggcg cctgatgtac
     7501 ggctccgagc cctgcactat ggccgggagt gtgccaatgg ttacctggaa ctgctggacc
     7561 acgtgctgct gacccttcag aagccaaacc cagacctgaa gcagcagcta actggacact
     7621 caaagcgggt tgctggctct gtgactgagc tcatccaggc tgctgaagcc atgaaaggaa
     7681 cagagtgggt ggacccagag gaccctactg tcattgctga gaatgagctc ttgggagccg
     7741 cagccgccat cgaggctgca gccaagaagc tggagcagct gaagcccagg gccaaaccca
     7801 aggaggcgga tgagtccttg aactttgagg aacaaatcct agaagctgcc aagtccatcg
     7861 ctgcagccac cagtgcactg gtaaaggctg cgtcagcagc ccagagggag ctggtggctc
     7921 aaggaaaggt gggcgccatt ccagccaatg cactggatga tgggcagtgg tcgcagggcc
     7981 tcatttccgc tgcccgtatg gtggctgcgg ccaccaacaa tctgtgtgaa gcagctaacg
     8041 cagctgtcca gggccatgct agccaggaga aactcatttc ctcagccaag caagtagctg
     8101 cctccacagc tcagctcctg gtagcttgca aggtcaaagc agatcaggac tctgaggcaa
     8161 tgaaacggct ccaggctgct ggcaatgcag tgaagagggc ctcagataac ctggtaaagg
     8221 cggcccagaa agctgcagcc ttcgaagacc aggagaatga gacggtggtg gtgaaggaga
     8281 aaatggttgg gggcattgcc cagattatcg cagcacagga agagatgctt cggaaggaac
     8341 gagagctgga agaggctcgg aaaaagctcg cccagatccg gcagcagcag tacaagttct
     8401 tgccttcaga gcttcgagac gagcactaaa aaagccctgt gtatttaatg cagacccagc
     8461 ccagagactg tgcctgccac taccaaagcc ttctgggctg ctcaggaccc aagctgccca
     8521 accccagcac tcccccaaag tgcctgccaa accctgggcc tggccctgcc cagtcccact
     8581 gcaagccgtg tcctctccct gactcccaag tgcctttgca ccctagggcc cctaagtgcc
     8641 tgcccccctc cagagtatta acgctccaag agtattatta atgctgccgt acctcagttt
     8701 gaacctgcca gggccccagc tgctccagcc tgccagcagc ttccagccag tccccacggc
     8761 cacgtcagct caactcatcc ctttttgata ctatatcccc tacccagcta cctatggggc
     8821 ttgagggttg taaacccaaa caggtcagac tcccggaatt ctgcagtcga cggtaccgcg
     8881 ggcccgggat ccaccggatc tagataactg atcataatca gccata
//
