LOCUS       sequence-88386-e        2609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 400 / 490, Emission: 468 
            / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2609)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-TBRII-N-18
FEATURES             Location/Qualifiers
     source          1..2609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1353..1364
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1853..2569
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 cgtcagatcc gctagcgcca ccatgggtcg ggggctgctc aggggcctgt ggccgctgca
       61 catcgtcctg tggacgcgta tcgccagcac gatcccaccg cacgttcaga agtcggatgt
      121 ggaaatggag gcccagaaag atgaaatcat ctgccccagc tgtaatagga ctgcccatcc
      181 actgagacat attaataacg acatgatagt cactgacaac aacggtgcag tcaagtttcc
      241 acaactgtgt aaattttgtg atgtgagatt ttccacctgt gacaaccaga aatcctgcat
      301 gagcaactgc agcatcacct ccatctgtga gaagccacag gaagtctgtg tggctgtatg
      361 gagaaagaat gacgagaaca taacactaga gacagtttgc catgacccca agctccccta
      421 ccatgacttt attctggaag atgctgcttc tccaaagtgc attatgaagg aaaaaaaaaa
      481 gcctggtgag actttcttca tgtgttcctg tagctctgat gagtgcaatg acaacatcat
      541 cttctcagaa gaatataaca ccagcaatcc tgacttgttg ctagtcatat ttcaagtgac
      601 aggcatcagc ctcctgccac cactgggagt tgccatatct gtcatcatca tcttctactg
      661 ctaccgcgtt aaccggcagc agaagctgag ttcaacctgg gaaaccggca agacgcggaa
      721 gctcatggag ttcagcgagc actgtgccat catcctggaa gatgaccgct ctgacatcag
      781 ctccacgtgt gccaacaaca tcaaccacaa cacagagctg ctgcccattg agctggacac
      841 cctggtgggg aaaggtcgct ttgctgaggt ctataaggcc aagctgaagc agaacacttc
      901 agagcagttt gagacagtgg cagtcaagat ctttccctat gaggagtatg cctcttggaa
      961 gacagagaag gacatcttct cagacatcaa tctgaagcat gagaacatac tccagttcct
     1021 gacggctgag gagcggaaga cggagttggg gaaacaatac tggctgatca ccgccttcca
     1081 cgccaagggc aacctacagg agtacctgac gcggcatgtc atcagctggg aggacctgcg
     1141 caagctgggc agctccctcg cccgggggat tgctaacctc cacagtgatc acactccatg
     1201 tgggaggccc aagatgccca tcgtgcacag ggacctcaag agctccaata tcctcgtgaa
     1261 gaacgaccta acctgctgcc tgtgtgactt tgggctttcc ctgcgtctgg accctactct
     1321 gtctgtggat gacctggcta acagtgggca ggtgggaact gcaagataca tggctccaga
     1381 agtcctagaa tccaggatga atttggagaa tgttgagtcc ttcaagcaga ccgatgtcta
     1441 ctccatggct ctggtgctct gggaaatgac atctcgctgt aatgcagtgg gagaagtaaa
     1501 agattatgag cctccatttg gttccaaggt gcgggagcac ccctgtgtcg aaagcatgaa
     1561 ggacaacgtg ttgagagatc gagggcgacc agaaattccc agcttctggc tcaaccacca
     1621 gggcatccag atggtgtgtg agacgttgac tgagtgctgg gaccacgacc cagaggcccg
     1681 tctcacagcc cagtgtgtgg cagaacgctt cagtgagctg gagcatctgg acaggctctc
     1741 ggggaggagc tgctcggagg agaagattcc tgaagacggc tccctaaaca ctaccaaaag
     1801 cagcggtgga gcaagcgcag ccagtggtag cgcggatcca ccggtcgcca ccatgagcaa
     1861 gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc gagctgaatg gcgatgtgaa
     1921 tggccacaag ttcagcgtga gcggcgaggg cgagggcgat gccacctacg gcaagctgac
     1981 cctgaagttc atctgcacca ccggcaagct gcctgtgccc tggcccaccc tggtggccac
     2041 cctgagctac ggcgtgcagt gcttctcacg ctaccccgat cacatgaagc agcacgactt
     2101 cttcaagagc gccatgcctg agggctacat ccaggagcgc accatcttct tcgaggatga
     2161 cggcaactac aagacgcgcg ccgaggtgaa gttcgagggc gataccctgg tgagtcgcat
     2221 cgagctgacc ggcactgatt tcaaggagga tggcaacatc ctgggcaata agatggagta
     2281 caactacaac gccaccaatg tgtacatcgt ggccgacaag gccaggaatg gcatcaaggt
     2341 gaacttcaag gtccgccaca acatcaagga tggcagcgtg cagctggccg accactacca
     2401 gcagaatacc cccatcggcg atggccctgt gctgctgccc gataaccact acctgtccac
     2461 ccagagcgcc ctgtccaagg accccaacga gaagcgcgat cacatgatct acctcgagtt
     2521 cgtgaccgcc gccgccatca cccacggcat ggatgagctg tacaagtaaa gcggccgcga
     2581 ctctagatca taatcagcca taccacatt
//
