LOCUS       sequence-88387-e        1245 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Outer Mitochondrial Membrane, Excitation: 400 / 490, 
            Emission: 468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1245)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-TOMM20-N-10
FEATURES             Location/Qualifiers
     source          1..1245
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             489..1205
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgcc accatggtgg gtcggaacag cgccatcgcc gccggtgtat
       61 gcggggccct tttcattggg tactgcatct acttcgaccg caaaagacga agtgacccca
      121 acttcaagaa caggcttcga gaacgaagaa agaaacagaa gcttgccaag gagagagctg
      181 ggctttccaa gttacctgac cttaaagatg ctgaagctgt tcagaagttc ttccttgaag
      241 aaatacagct tggtgaagag ttactagctc aaggtgaata tgagaagggc gtagaccatc
      301 tgacaaatgc aattgctgtg tgtggacagc cacagcagtt actgcaggtc ttacagcaaa
      361 ctcttccacc accagtgttc cagatgcttc tgactaagct cccaacaatt agtcagagaa
      421 ttgtaagtgc tcagagcttg gctgaagatg atgtggaagg cggtagcggg gatccaccgg
      481 tcgccaccat gagcaagggc gccgagctgt tcaccggcat cgtgcccatc ctgatcgagc
      541 tgaatggcga tgtgaatggc cacaagttca gcgtgagcgg cgagggcgag ggcgatgcca
      601 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgcct gtgccctggc
      661 ccaccctggt ggccaccctg agctacggcg tgcagtgctt ctcacgctac cccgatcaca
      721 tgaagcagca cgacttcttc aagagcgcca tgcctgaggg ctacatccag gagcgcacca
      781 tcttcttcga ggatgacggc aactacaaga cgcgcgccga ggtgaagttc gagggcgata
      841 ccctggtgag tcgcatcgag ctgaccggca ctgatttcaa ggaggatggc aacatcctgg
      901 gcaataagat ggagtacaac tacaacgcca ccaatgtgta catcgtggcc gacaaggcca
      961 ggaatggcat caaggtgaac ttcaaggtcc gccacaacat caaggatggc agcgtgcagc
     1021 tggccgacca ctaccagcag aataccccca tcggcgatgg ccctgtgctg ctgcccgata
     1081 accactacct gtccacccag agcgccctgt ccaaggaccc caacgagaag cgcgatcaca
     1141 tgatctacct cgagttcgtg accgccgccg ccatcaccca cggcatggat gagctgtaca
     1201 agtaaagcgg ccgcgactct agatcataat cagccatacc acatt
//
