LOCUS       sequence-88391-e        4047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 400 / 490, Emission: 468 
            / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4047)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..4047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..750
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 gtgacgtcag atccgctagc gctaccggtc gccaccatga gcaagggcgc cgagctgttc
       61 accggcatcg tgcccatcct gatcgagctg aatggcgatg tgaatggcca caagttcagc
      121 gtgagcggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcctgt gccctggccc accctggtgg ccaccctgag ctacggcgtg
      241 cagtgcttct cacgctaccc cgatcacatg aagcagcacg acttcttcaa gagcgccatg
      301 cctgagggct acatccagga gcgcaccatc ttcttcgagg atgacggcaa ctacaagacg
      361 cgcgccgagg tgaagttcga gggcgatacc ctggtgagtc gcatcgagct gaccggcact
      421 gatttcaagg aggatggcaa catcctgggc aataagatgg agtacaacta caacgccacc
      481 aatgtgtaca tcgtggccga caaggccagg aatggcatca aggtgaactt caaggtccgc
      541 cacaacatca aggatggcag cgtgcagctg gccgaccact accagcagaa tacccccatc
      601 ggcgatggcc ctgtgctgct gcccgataac cactacctgt ccacccagag cgccctgtcc
      661 aaggacccca acgagaagcg cgatcacatg atctacctcg agttcgtgac cgccgccgcc
      721 atcacccacg gcatggatga gctgtacaag tccggactca gatctcgagc tcaagcttcg
      781 aattcgggaa actcgagaac aaagcttgaa aacggccgca tgccagtgtt tcatacgcgc
      841 acgatcgaga gcatcctgga gccggtggca cagcagatct cccacctggt gataatgcac
      901 gaggagggcg aggtggacgg caaagccatt cctgacctca ccgcgcccgt ggccgccgtg
      961 caggcggccg tcagcaacct cgtccgggtt ggaaaagaga ctgttcaaac cactgaggat
     1021 cagattttga agagagatat gccaccagca tttattaagg ttgagaatgc ttgcaccaag
     1081 cttgtccagg cagctcagat gcttcagtca gacccttact cagtgcctgc tcgagattat
     1141 ctaattgatg ggtcaagggg catcctctct ggaacatcag acctgctcct taccttcgat
     1201 gaggctgagg tccgtaaaat tattagagtt tgcaaaggaa ttttggaata tcttacagtg
     1261 gcagaggtgg tggagactat ggaagatttg gtcacttaca caaagaatct tgggccagga
     1321 atgactaaga tggccaagat gattgacgag agacagcagg agctcactca ccaggagcac
     1381 cgagtgatgt tggtgaactc gatgaacacc gtgaaagagt tgctgccagt tctcatttca
     1441 gctatgaaga tttttgtaac aactaaaaac tcaaaaaacc aaggcataga ggaagcttta
     1501 aaaaatcgca attttactgt agaaaaaatg agtgctgaaa ttaatgagat aattcgtgtg
     1561 ttacaactca cctcttggga tgaagatgcc tgggccagca aggacactga agccatgaag
     1621 agagcattgg cctccataga ctccaaactg aaccaggcca aaggttggct ccgtgaccct
     1681 agtgcctccc caggggatgc tggtgagcag gccatcagac agatcttaga tgaagctgga
     1741 aaagttggtg aactctgtgc aggcaaagaa cgcagggaga ttctgggaac ttgcaaaatg
     1801 ctagggcaga tgactgatca agtggctgac ctccgtgcca gaggacaagg atcctcaccg
     1861 gtggccatgc agaaagctca gcaggtatct cagggtctgg atgtgctcac agcaaaagtg
     1921 gaaaatgcag ctcgcaagct ggaagccatg accaactcaa agcagagcat tgcaaagaag
     1981 atcgatgctg ctcagaactg gcttgcagat ccaaatggtg gaccggaagg agaagagcag
     2041 attcgaggtg ctttggctga agctcggaaa atagcagaat tatgtgatga tcctaaagaa
     2101 agagatgaca ttctacgttc ccttggggaa atatctgctc tgacttctaa attagcagat
     2161 ctacgaagac aggggaaagg agattctcca gaggctcgag ccttggccaa acaggtggcc
     2221 acggccctgc agaacctgca gaccaaaacc aaccgggctg tggccaacag cagaccggcc
     2281 aaagcagctg tacaccttga gggcaagatt gagcaagcac agcggtggat tgataatccc
     2341 acagtggatg accgtggagt cggtcaggct gccatccggg ggcttgtggc cgaagggcat
     2401 cgtctggcta atgttatgat ggggccttat cggcaagatc ttctcgccaa gtgtgaccga
     2461 gtggaccagc tgacagccca gctggctgac ctggctgcca gaggggaagg ggagagtcct
     2521 caggcacgag cacttgcatc tcagctccaa gactccttaa aggatctaaa agctcggatg
     2581 caggaggcca tgactcagga agtgtcagat gttttcagcg ataccacaac tcccatcaag
     2641 ctgttggcag tggcagccac ggcgcctcct gatgcgccta acagggaaga ggtatttgat
     2701 gagagggcag ctaactttga aaaccattca ggaaagcttg gtgctacggc cgagaaggcg
     2761 gctgcggttg gtactgctaa taaatcaaca gtggaaggca ttcaggcctc agtgaagacg
     2821 gcccgagaac tcacacccca ggtggtctcg gctgctcgta tcttacttag gaaccctgga
     2881 aatcaagctg cttatgaaca ttttgagacc atgaagaacc agtggatcga taatgttgaa
     2941 aaaatgacag ggctggtgga cgaagccatt gataccaaat ctctgttgga tgcttcagaa
     3001 gaagcaatta aaaaagacct ggacaagtgc aaggtagcta tggccaacat tcagcctcag
     3061 atgctggttg ctggggcaac cagtattgct cgtcgggcca accggatcct gctggtggct
     3121 aagagggagg tggagaattc cgaggatccc aagttccgtg aggctgtgaa agctgcctct
     3181 gatgaattga gcaaaaccat ctccccgatg gtgatggatg caaaagctgt ggctggaaac
     3241 atttccgacc ctggactgca aaagagcttc ctggactcag gatatcggat cctgggagct
     3301 gtggccaagg tcagagaagc cttccaacct caggagcctg acttcccgcc gcctccacca
     3361 gaccttgaac aactccgact aacagatgag cttgctcctc ccaaaccacc tctgcctgaa
     3421 ggtgaggtcc ctccacctag gcctccacca ccagaggaaa aggatgaaga gttccctgag
     3481 cagaaggccg gggaggtgat taaccagcca atgatgatgg ctgccagaca gctccatgat
     3541 gaagctcgca aatggtccag caagggcaat gacatcattg cagcagccaa gcgcatggct
     3601 ctgctgatgg ctgagatgtc tcggctggta agagggggca gtggtaccaa gcgggcactc
     3661 attcagtgtg ccaaggacat cgccaaggcc tcagatgagg tgactcggtt ggccaaggag
     3721 gttgccaagc agtgcacaga taaacggatt agaaccaacc tcttacaggt atgtgagcga
     3781 atcccaacca taagcaccca gctcaaaatc ctgtccacag tgaaggccac catgctgggc
     3841 cggaccaaca tcagtgatga ggagtctgag caggccacag agatgctggt tcacaatgcc
     3901 cagaacctca tgcagtctgt gaaggagact gtgcgggaag ctgaagctgc ttcaatcaaa
     3961 attcgaacag atgctggatt tacactgcgc tgggttagaa agactccctg gtaccagtag
     4021 gcggccgcga ctctagatca taatcag
//
