LOCUS       sequence-88393-e        2553 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 400 / 490, Emission: 468 
            / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2553)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2553
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      61..70
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1797..2513
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 ctagtgacgt cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
       61 ccatggcggc cccccgcccg tctcccgcga tccccgtttc ggtctcggct ccggcttttt
      121 acgccccgca gaagaagttc ggccctgtgg tggccccaaa gcccaaagtg aatcccttcc
      181 ggcccaggga cagcgagcct cccccggcac ccggggccca gcgcgcacag atgggccggg
      241 tgggcgatat tcccccgccg cccccggaag actttcccct gcctccacct ccccttgctg
      301 gggatggcga cgatgcagag ggtgctctgg gaggtgcctt cccgccgccc cctcccccga
      361 tcgaggaatc atttccccct gcgcctctgg aggaggagat cttcccttcc ccgccgcctc
      421 ctccggagga ggagggaggg cctgaggccc ccataccgcc cccaccacag cccagggaga
      481 aggtgagcag tattgatttg gagatcgact ctctgtcctc actgctggat gacatgacca
      541 agaatgatcc tttcaaagcc cgggtgtcat ctggatatgt gcccccacca gtggccactc
      601 cattcagttc caagtccagt accaagcctg cagccggggg cacagcaccc ctgcctcctt
      661 ggaagtcccc ttccagctcc cagcctctgc cccaggttcc ggctccggct cagagccaga
      721 cacagttcca tgttcagccc cagccccagc ccaagcctca ggtccaactc catgtccagt
      781 cccagaccca gcctgtgtct ttggctaaca cccagccccg agggccccca gcctcatctc
      841 cggctccagc ccctaagttt tctccagtga ctcctaagtt tactcctgtg gcttccaagt
      901 tcagtcctgg agccccaggt ggatctgggt cacaaccaaa tcaaaaattg gggcaccccg
      961 aagctctttc tgctggcaca ggctcccctc aacctcccag cttcacctat gcccagcaga
     1021 gggagaagcc ccgagtgcag gagaagcagc accccgtgcc cccaccggct cagaaccaaa
     1081 accaggtgcg ctcccctggg gccccagggc ccctgactct gaaggaggtg gaggagctgg
     1141 agcagctgac ccagcagcta atgcaggaca tggagcatcc tcagaggcag aatgtggctg
     1201 tcaacgaact ctgcggccga tgccatcaac ccctggcccg ggcgcagcca gccgtccgcg
     1261 ctctagggca gctgttccac atcgcctgct tcacctgcca ccagtgtgcg cagcagctcc
     1321 agggccagca gttctacagt ctggaggggg cgccgtactg cgagggctgt tacactgaca
     1381 ccctggagaa gtgtaacacc tgcggggagc ccatcactga ccgcatgctg agggccacgg
     1441 gcaaggccta tcacccgcac tgcttcacct gtgtggtctg cgcccgcccc ctggagggca
     1501 cctccttcat cgtggaccag gccaaccggc cccactgtgt ccccgactac cacaagcagt
     1561 acgccccgag gtgctccgtc tgctctgagc ccatcatgcc tgagcctggc cgagatgaga
     1621 ctgtgcgagt ggtcgccctg gacaagaact tccacatgaa gtgttacaag tgtgaggact
     1681 gcgggaagcc cctgtcgatt gaggcagatg acaatggctg cttccccctg gacggtcacg
     1741 tgctctgtcg gaagtgccac actgctagag cccagacgga tccaccggtc gccaccatga
     1801 gcaagggcgc cgagctgttc accggcatcg tgcccatcct gatcgagctg aatggcgatg
     1861 tgaatggcca caagttcagc gtgagcggcg agggcgaggg cgatgccacc tacggcaagc
     1921 tgaccctgaa gttcatctgc accaccggca agctgcctgt gccctggccc accctggtgg
     1981 ccaccctgag ctacggcgtg cagtgcttct cacgctaccc cgatcacatg aagcagcacg
     2041 acttcttcaa gagcgccatg cctgagggct acatccagga gcgcaccatc ttcttcgagg
     2101 atgacggcaa ctacaagacg cgcgccgagg tgaagttcga gggcgatacc ctggtgagtc
     2161 gcatcgagct gaccggcact gatttcaagg aggatggcaa catcctgggc aataagatgg
     2221 agtacaacta caacgccacc aatgtgtaca tcgtggccga caaggccagg aatggcatca
     2281 aggtgaactt caaggtccgc cacaacatca aggatggcag cgtgcagctg gccgaccact
     2341 accagcagaa tacccccatc ggcgatggcc ctgtgctgct gcccgataac cactacctgt
     2401 ccacccagag cgccctgtcc aaggacccca acgagaagcg cgatcacatg atctacctcg
     2461 agttcgtgac cgccgccgcc atcacccacg gcatggatga gctgtacaag taaagcggcc
     2521 gcgactctag atcataatca gccataccac att
//
