LOCUS       sequence-88396-e        1927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1927)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..710
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 gaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagtgtgatt aaaccagaca
       61 tgaagatcaa gctgcgtatg gaaggcgctg taaatggaca cccgttcgcg attgaaggag
      121 ttggccttgg gaagcctttc gagggaaaac agagtatgga ccttaaagtc aaagaaggcg
      181 gacctctgcc tttcgcctat gacatcttga caactgtgtt ctgttacggc aacagggtat
      241 tcgccaaata cccagaaaat atagtagact atttcaagca gtcgtttcct gagggctact
      301 cttgggaacg aagcatgaat tacgaagacg ggggcatttg taacgcgaca aacgacataa
      361 ccctggatgg tgactgttat atctatgaaa ttcgatttga tggtgtgaac tttcctgcca
      421 atggtccagt tatgcagaag aggactgtga aatgggagcc atccactgag aaattgtatg
      481 tgcgtgatgg agtgctgaag ggtgatgtta acatggctct gtcgcttgaa ggaggtggcc
      541 attaccgatg tgacttcaaa actacttata aagctaagaa ggttgtccag ttgccagact
      601 atcactttgt ggaccaccac attgagatta aaagccacga caaagattac agtaatgtta
      661 atctgcatga gcacgccgaa gcgcattctg agctgccgag gcaggccaag tccggactca
      721 gatctggcag cggtggaggc agcgcatccg gcggaagcgg aagcgatgat gatatcgccg
      781 cgctcgtcgt cgacaacggc tccggcatgt gcaaggccgg cttcgcgggc gacgatgccc
      841 cccgggccgt cttcccctcc atcgtggggc gccccaggca ccagggcgtg atggtgggca
      901 tgggtcagaa ggattcctat gtgggcgacg aggcccagag caagagaggc atcctcaccc
      961 tgaagtaccc catcgagcac ggcatcgtca ccaactggga cgacatggag aaaatctggc
     1021 accacacctt ctacaatgag ctgcgtgtgg ctcccgagga gcaccccgtg ctgctgaccg
     1081 aggcccccct gaaccccaag gccaaccgcg agaagatgac ccagatcatg tttgagacct
     1141 tcaacacccc agccatgtac gttgctatcc aggctgtgct atccctgtac gcctctggcc
     1201 gtaccactgg catcgtgatg gactccggtg acggggtcac ccacactgtg cccatctacg
     1261 aggggtatgc cctcccccat gccatcctgc gtctggacct ggctggccgg gacctgactg
     1321 actacctcat gaagatcctc accgagcgcg gctacagctt caccaccacg gccgagcggg
     1381 aaatcgtgcg tgacattaag gagaagctgt gctacgtcgc cctggacttc gagcaagaga
     1441 tggccacggc tgcttccagc tcctccctgg agaagagcta cgagctgcct gacggccagg
     1501 tcatcaccat tggcaatgag cggttccgct gccctgaggc actcttccag ccttccttcc
     1561 tgggcatgga gtcctgtggc atccacgaaa ctaccttcaa ctccatcatg aagtgtgacg
     1621 tggacatccg caaagacctg tacgccaaca cagtgctgtc tggcggcacc accatgtacc
     1681 ctggcattgc cgacaggatg cagaaggaga tcactgccct ggcacccagc acaatgaaga
     1741 tcaagatcat tgctcctcct gagcgcaagt actccgtgtg gatcggcggc tccatcctgg
     1801 cctcgctgtc caccttccag cagatgtgga tcagcaagca ggagtatgac gagtccggcc
     1861 cctccatcgt ccaccgcaaa tgcttctagg gatccaccgg atctagataa ctgatcataa
     1921 tcagcca
//
