LOCUS       sequence-88397-e        3493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Alpha Actinin, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3493)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Alpha-Actinin-19
FEATURES             Location/Qualifiers
     source          1..3493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2771..2780
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2777..3454
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gcaccatcat
       61 ggaccattat gattctcagc aaaccaacga ttacatgcag ccagaagagg actgggaccg
      121 ggacctgctc ctggacccgg cctgggagaa gcagcagaga aagacattca cggcatggtg
      181 taactcccac ctccggaagg cggggacaca gatcgagaac atcgaagagg acttccggga
      241 tggcctgaag ctcatgctgc tgctggaggt catctcaggt gaacgcttgg ccaagccaga
      301 gcgaggcaag atgagagtgc acaagatctc caacgtcaac aaggccctgg atttcatagc
      361 cagcaaaggc gtcaaactgg tgtccatcgg agccgaagaa atcgtggatg ggaatgtgaa
      421 gatgaccctg ggcatgatct ggaccatcat cctgcgcttt gccatccagg acatctccgt
      481 ggaagagact tcagccaagg aagggctgct cctgtggtgt cagagaaaga cagcccctta
      541 caaaaatgtc aacatccaga acttccacat aagctggaag gatggcctcg gcttctgtgc
      601 tttgatccac cgacaccggc ccgagctgat tgactacggg aagctgcgga aggatgatcc
      661 actcacaaat ctgaatacgg cttttgacgt ggcagagaag tacctggaca tccccaagat
      721 gctggatgcc gaagacatcg ttggaactgc ccgaccggat gagaaagcca tcatgactta
      781 cgtgtctagc ttctaccacg ccttctctgg agcccagaag gcggagacag cagccaatcg
      841 catctgcaag gtgttggccg tcaaccagga gaacgagcag cttatggaag actacgagaa
      901 gctggccagt gatctgttgg agtggatccg ccgcacaatc ccgtggctgg agaaccgggt
      961 gcccgagaac accatgcatg ccatgcaaca gaagctggag gacttccggg actaccggcg
     1021 cctgcacaag ccgcccaagg tgcaggagaa gtgccagctg gagatcaact tcaacacgct
     1081 gcagaccaag ctgcggctca gcaaccggcc tgccttcatg ccctctgagg gcaggatggt
     1141 ctcggacatc aacaatgcct ggggctgcct ggagcaggtg gagaagggct atgaggagtg
     1201 gttgctgaat gagatccgga ggctggagcg actggaccac ctggcagaga agttccggca
     1261 gaaggcctcc atccacgagg cctggactga cggcaaagag gccatgctgc gacagaagga
     1321 ctatgagacc gccaccctct cggagatcaa ggccttgctc aagaagcatg aggccttcga
     1381 gagtgacctg gctgcccacc aggaccgtgt ggagcagatt gccgccatcg cacaggagct
     1441 caatgagctg gactattatg actcacccag tgtcaacgcc cgttgccaaa agatctgtga
     1501 ccagtgggac aatctggggg ccctaactca gaagcgaagg gaagctctgg agcggaccga
     1561 gaaactgctg gagaccattg accagctgta cttggagtat gccaagcggg ctgcaccctt
     1621 caacaactgg atggaggggg ccatggagga cctgcaggac accttcattg tgcacaccat
     1681 tgaggagatc cagggactga ccacagccca tgagcagttc aaggccaccc tccctgatgc
     1741 cgacaaggag cgcctggcca tcctgggcat ccacaatgag gtgtccaaga ttgtccagac
     1801 ctaccacgtc aatatggcgg gcaccaaccc ctacacaacc atcacgcctc aggagatcaa
     1861 tggcaaatgg gaccacgtgc ggcagctggt gcctcggagg gaccaagctc tgacggagga
     1921 gcatgcccga cagcagcaca atgagaggct acgcaagcag tttggagccc aggccaatgt
     1981 catcgggccc tggatccaga ccaagatgga ggagatcggg aggatctcca ttgagatgca
     2041 tgggaccctg gaggaccagc tcagccacct gcggcagtat gagaagagca tcgtcaacta
     2101 caagccaaag attgatcagc tggagggcga ccaccagctc atccaggagg cgctcatctt
     2161 cgacaacaag cacaccaact acaccatgga gcacatccgt gtgggctggg agcagctgct
     2221 caccaccatc gccaggacca tcaatgaggt agagaaccag atcctgaccc gggatgccaa
     2281 gggcatcagc caggagcaga tgaatgagtt ccgggcctcc ttcaaccact ttgaccggaa
     2341 gaagacaggc atgatggaca cggatgattt ccgcgcctgc ctgatctcca tgggttacaa
     2401 catgggagaa gcagaatttg cccgcatcat gagcattgtg gaccccaacc gcctgggggt
     2461 agtgacattc caggccttca ttgacttcat gtcccgcgag acagccgaca cagatacagc
     2521 agaccaagtc atggcttcct tcaagatcct ggctggggac aagaactaca ttaccatgga
     2581 cgagctgcgc cgcgagctgc cacccgacca ggctgagtac tgcatcgcgc ggatggcccc
     2641 ctacaccggc cccgactccg tgccaggtgc tctggactac atgtccttct ccacggcgct
     2701 gtacggcgag agtgacctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga
     2761 tccaccggtc gccaccatgg tgagtgtgat taaaccagac atgaagatca agctgcgtat
     2821 ggaaggcgct gtaaatggac acccgttcgc gattgaagga gttggccttg ggaagccttt
     2881 cgagggaaaa cagagtatgg accttaaagt caaagaaggc ggacctctgc ctttcgccta
     2941 tgacatcttg acaactgtgt tctgttacgg caacagggta ttcgccaaat acccagaaaa
     3001 tatagtagac tatttcaagc agtcgtttcc tgagggctac tcttgggaac gaagcatgaa
     3061 ttacgaagac gggggcattt gtaacgcgac aaacgacata accctggatg gtgactgtta
     3121 tatctatgaa attcgatttg atggtgtgaa ctttcctgcc aatggtccag ttatgcagaa
     3181 gaggactgtg aaatgggagc catccactga gaaattgtat gtgcgtgatg gagtgctgaa
     3241 gggtgatgtt aacatggctc tgtcgcttga aggaggtggc cattaccgat gtgacttcaa
     3301 aactacttat aaagctaaga aggttgtcca gttgccagac tatcactttg tggaccacca
     3361 cattgagatt aaaagccacg acaaagatta cagtaatgtt aatctgcatg agcacgccga
     3421 agcgcattct gagctgccga ggcaggccaa gtaatgatag gcggccgcga ctctagatca
     3481 taatcagcca tac
//
