LOCUS       sequence-88399-e        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Alpha-V-Integrin-25
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    250..302
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      312..321
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             318..995
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 ttgaggatat caccaactcc acattggtta ccactaatgt cacctggggc attcagccag
       61 cgcccatgcc tgtgcctgtg tgggtgatca ttttagcagt tctagcagga ttgttgctac
      121 tggctgtttt ggtatttgta atgtacagga tgggcttttt taaacgggtc cggccacctc
      181 aagaagaaca agaaagggag cagcttcaac ctcatgaaaa tggtgaagga aactcagaaa
      241 ctccgggatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
      301 ccccaccggt cgccaccatg gtgagtgtga ttaaaccaga catgaagatc aagctgcgta
      361 tggaaggcgc tgtaaatgga cacccgttcg cgattgaagg agttggcctt gggaagcctt
      421 tcgagggaaa acagagtatg gaccttaaag tcaaagaagg cggacctctg cctttcgcct
      481 atgacatctt gacaactgtg ttctgttacg gcaacagggt attcgccaaa tacccagaaa
      541 atatagtaga ctatttcaag cagtcgtttc ctgagggcta ctcttgggaa cgaagcatga
      601 attacgaaga cgggggcatt tgtaacgcga caaacgacat aaccctggat ggtgactgtt
      661 atatctatga aattcgattt gatggtgtga actttcctgc caatggtcca gttatgcaga
      721 agaggactgt gaaatgggag ccatccactg agaaattgta tgtgcgtgat ggagtgctga
      781 agggtgatgt taacatggct ctgtcgcttg aaggaggtgg ccattaccga tgtgacttca
      841 aaactactta taaagctaag aaggttgtcc agttgccaga ctatcacttt gtggaccacc
      901 acattgagat taaaagccac gacaaagatt acagtaatgt taatctgcat gagcacgccg
      961 aagcgcattc tgagctgccg aggcaggcca agtaatgata ggcggccgcg actctagatc
     1021 ataatcagcc ataccac
//
