LOCUS       sequence-88400-e        3142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3142)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Beta-Catenin-20
FEATURES             Location/Qualifiers
     source          1..3142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..708
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     regulatory      763..772
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             814..849
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 ccgtcagatc cgctagcgct accggtcgcc accatggtga gtgtgattaa accagacatg
       61 aagatcaagc tgcgtatgga aggcgctgta aatggacacc cgttcgcgat tgaaggagtt
      121 ggccttggga agcctttcga gggaaaacag agtatggacc ttaaagtcaa agaaggcgga
      181 cctctgcctt tcgcctatga catcttgaca actgtgttct gttacggcaa cagggtattc
      241 gccaaatacc cagaaaatat agtagactat ttcaagcagt cgtttcctga gggctactct
      301 tgggaacgaa gcatgaatta cgaagacggg ggcatttgta acgcgacaaa cgacataacc
      361 ctggatggtg actgttatat ctatgaaatt cgatttgatg gtgtgaactt tcctgccaat
      421 ggtccagtta tgcagaagag gactgtgaaa tgggagccat ccactgagaa attgtatgtg
      481 cgtgatggag tgctgaaggg tgatgttaac atggctctgt cgcttgaagg aggtggccat
      541 taccgatgtg acttcaaaac tacttataaa gctaagaagg ttgtccagtt gccagactat
      601 cactttgtgg accaccacat tgagattaaa agccacgaca aagattacag taatgttaat
      661 ctgcatgagc acgccgaagc gcattctgag ctgccgaggc aggccaagtc cggactcaga
      721 tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cggccaccat ggctactcaa
      781 gctgacctga tggagttgga catggccatg gagccggaca gaaaagctgc tgtcagccac
      841 tggcagcagc agtcttactt ggattctgga atccattctg gtgccaccac cacagctcct
      901 tccctgagtg gcaagggcaa ccctgaggaa gaagatgttg acacctccca agtcctttat
      961 gaatgggagc aaggcttttc ccagtccttc acgcaagagc aagtagctga tattgacggg
     1021 cagtatgcaa tgactagggc tcagagggtc cgagctgcca tgttccctga gacgctagat
     1081 gagggcatgc agatcccatc cacgcagttt gacgctgctc atcccactaa tgtccagcgc
     1141 ttggctgaac catcacagat gttgaaacat gcagttgtca atttgattaa ctatcaggat
     1201 gacgcggaac ttgccacacg tgcaattcct gagctgacaa aactgctaaa cgatgaggac
     1261 caggtggtag ttaataaagc tgctgttatg gtccatcagc tttccaaaaa ggaagcttcc
     1321 agacatgcca tcatgcgctc ccctcagatg gtgtctgcca ttgtacgcac catgcagaat
     1381 acaaatgatg tagagacagc tcgttgtact gctgggactc tgcacaacct ttctcaccac
     1441 cgcgagggct tgctggccat ctttaagtct ggtggcatcc cagcgctggt gaaaatgctt
     1501 gggtcaccag tggattctgt actgttctac gccatcacga cactgcataa tctcctgctc
     1561 catcaggaag gagctaaaat ggcagtgcgc ctagctggtg gactgcagaa aatggttgct
     1621 ttgctcaaca aaacaaacgt gaaattcttg gctattacaa cagactgcct tcagatctta
     1681 gcttatggca atcaagagag caagctcatc attctggcca gtggtggacc ccaagcctta
     1741 gtaaacataa tgaggaccta cacttatgag aagcttctgt ggaccacaag cagagtgctg
     1801 aaagtgctgt ctgtctgctc tagcaacaag ccggccattg tagaagctgg tgggatgcag
     1861 gcactggggc ttcatctgac agacccaagt cagcgacttg ttcaaaactg tctttggact
     1921 ctcagaaacc tttcagatgc agcgactaag caggaaggga tggaaggcct ccttgggact
     1981 ctagtgcagc ttctgggttc cgatgatata aatgtggtca cctgtgcagc tggaattctc
     2041 tctaacctca cttgcaataa ttacaaaaac aagatgatgg tgtgccaagt gggtggcata
     2101 gaggctcttg tacgcaccgt ccttcgtgct ggtgacaggg aagacatcac tgagcctgcc
     2161 atctgtgctc ttcgtcatct gaccagccgg catcaggaag ccgagatggc ccagaatgcc
     2221 gttcgccttc attatggact gcctgttgtg gttaaactcc tgcacccacc atcccactgg
     2281 cctctgataa aggcaactgt tggattgatt cgaaaccttg ccctttgccc agcaaatcat
     2341 gcgcctttgc gggaacaggg tgctattcca cgactagttc agctgcttgt acgagcacat
     2401 caggacaccc aacggcgcac ctccatgggt ggaacgcagc agcagtttgt ggagggcgtg
     2461 cgcatggagg agatagtaga agggtgtact ggagctctcc acatccttgc tcgggacgtt
     2521 cacaaccgga ttgtaatccg aggactcaat accattccat tgtttgtgca gttgctttat
     2581 tctcccattg aaaatatcca aagagtagct gcaggggtcc tctgtgaact tgctcaggac
     2641 aaggaggctg cagaggccat tgaagctgag ggagccacag ctcccctgac agagttactc
     2701 cactccagga atgaaggcgt ggcaacatac gcagctgctg tcctattccg aatgtctgag
     2761 gacaagccac aggattacaa gaagcggctt tcagtcgagc tgaccagttc cctcttcagg
     2821 acagagccaa tggcttggaa tgagactgca gatcttggac tggacattgg tgcccaggga
     2881 gaagcccttg gatatcgcca ggatgatccc agctaccgtt cttttcactc tggtggatac
     2941 ggccaggatg ccttggggat ggaccctatg atggagcatg agatgggtgg ccaccaccct
     3001 ggtgctgact atccagttga tgggctgcct gatctgggac acgcccagga cctcatggat
     3061 gggctgcccc caggtgatag caatcagctg gcctggtttg atactgacct gtaaaagctt
     3121 ggatccaccg gatctagata ac
//
