LOCUS       sequence-88401-e        3518 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 503, 
            Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3518)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..3518
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..708
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     protein_bind    2527..2538
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gtgtgattaa accagacatg
       61 aagatcaagc tgcgtatgga aggcgctgta aatggacacc cgttcgcgat tgaaggagtt
      121 ggccttggga agcctttcga gggaaaacag agtatggacc ttaaagtcaa agaaggcgga
      181 cctctgcctt tcgcctatga catcttgaca actgtgttct gttacggcaa cagggtattc
      241 gccaaatacc cagaaaatat agtagactat ttcaagcagt cgtttcctga gggctactct
      301 tgggaacgaa gcatgaatta cgaagacggg ggcatttgta acgcgacaaa cgacataacc
      361 ctggatggtg actgttatat ctatgaaatt cgatttgatg gtgtgaactt tcctgccaat
      421 ggtccagtta tgcagaagag gactgtgaaa tgggagccat ccactgagaa attgtatgtg
      481 cgtgatggag tgctgaaggg tgatgttaac atggctctgt cgcttgaagg aggtggccat
      541 taccgatgtg acttcaaaac tacttataaa gctaagaagg ttgtccagtt gccagactat
      601 cactttgtgg accaccacat tgagattaaa agccacgaca aagattacag taatgttaat
      661 ctgcatgagc acgccgaagc gcattctgag ctgccgaggc aggccaagtc cggactcaga
      721 tctggcagcg gtggaggctt ggaggagccg gaggccgcga cgcggacagc cgcggccgtg
      781 gattgcaaag acagacccgg gttcccagtg aagaggttaa tacaagcccg cttgcccttc
      841 aaacgcctga accttgttcc caaggagaaa gttgaggaag acacatcacc aaaagcagct
      901 gtggaaagca aagtcccgga tctacaattg tctttgggca cttttgaaag ccagtgtcac
      961 acaggctctc acgtaggctt gagtacaaag ttggtcggtg gccagggccc cattgatagc
     1021 ttcttgaggg caacaatcaa gccagtaccg agtgtggtca ttatcgacct gacggagaac
     1081 tgcagtgaca ttccagacag ccctgagggc cacagtgaac taagtcctga cactgctggt
     1141 gtggtcacca cagtggaagg agctgccaag cagcaggagc actctgcagc agagctgtgt
     1201 ctcctagaga ccccttcaga catcacttgt cacatggaag aggagcctgg gagtccagga
     1261 gacccaaaga ggacgggtga ctgtcaggct ggttcactac agagctgtcc ggagctgacc
     1321 ccaggttcta ggacatgccc caccaaggag ttatcaagct ggagcaaggc aggggatctc
     1381 ctatttatag agaaagtgcc tgtggtggtg ctagaggaca tcctggccac taagccctcc
     1441 attgcctctc ttcccatgat gtccctggat aggagtgtca cttcagagag tgaaatactg
     1501 gagtcctgcc ccgaggatga ctccatactg agccactcct ccaccaactc ctcttctcca
     1561 accagctctc ctgagggacc atccacaccc ccagaacatc ggggtgggag aagctccccc
     1621 tccaccccag cctgcagagt tgccaagaac tttgtcaaag gctccacaga gaagggcagg
     1681 agcaaactgc acagagatag agaacagcag agggaagaga aggaaaagct gagggaagag
     1741 atacgacgag ccaaggagga ggcacggaag aagaaggagg aggagaagga gctgaaggaa
     1801 aaggagcgga gggaaaagcg ggagaaagat gagaaggaga aggcggagaa gcagcggcta
     1861 aaggaggaaa aacgcaagga gcggcaggaa gctctggagg ctaaactgga ggagaagagg
     1921 aagaaggagg aagagaagcg gttacgggag gaagagaagc ggttacggga agaagagaag
     1981 cgcattaagg cagaaaaggc agagatcact cgattcttcc agaaaccgaa gactccgcag
     2041 gctcccaaga ccctggccgg ttcctgtggt aagtttgccc cctttgaaat caaagaacac
     2101 atggtgctgg ctccacggtg ccgagctgcc ttggatcaag acctgtgcga tcagctggac
     2161 cagctcctgc agcagcagag tgtggcaagc accttcctca gtgacctaaa aagccggctg
     2221 cccttgagat cggggcccac ccgggtctgt ggccatgaca cagacatcat gaacagggat
     2281 gtggtcattg tggagagcag caaggtggac ggtgtgtctg aaaggaagaa gtttggccgc
     2341 atgaagcttc tgcagttctc agagaaccac cggccagcat actgggggac gtggaacaag
     2401 aagacagcca tcatccgccc ccggaacccc tgggcccagg acaaagacct tcttgactat
     2461 gaggtagaca gtgacgatga gtgggaagag gaggaacctg gggagtcttt gtcccacagt
     2521 gagggggatg aggatgacga tgtgggggaa gatgaggatg aggacgatgg cttctttgtg
     2581 ccccatgggt acttatctga agatgaaggc gtgactgagg agtgtgcaga ccctgagaac
     2641 cacaaagtac accagaagct gaaggccaag gagtgggatg agctcctagc caagggcaag
     2701 cgctttcgtg tgctgcagcc tgtgcacgtg ggatgtgtat gggcatcaga ggcagccaac
     2761 tgcacgagtt ccgacttgaa gctgctgcag cagttcactg catgcctgct ggatgtggca
     2821 tcccctgatg agccagaacc cggggcctcc aggagggaaa agagggacca gcatatcctg
     2881 gcccagttgc ttccgctgct acatggcaat gtgaatggga gcaaggtgat catccatgaa
     2941 ttccaggagc aatgccgcag gggcctgctg acccttccaa gtcccacccc acacctgcag
     3001 atgcccaact tggaggatgc tgttgctgtg ccatccaagg ccaggctgaa gcgccttatc
     3061 tctgagaact ctgcctatga gaagcggccc aacttccgta tgtgctggta tgtgcaccca
     3121 gaggtgctca agagctttgg ccaggagtgc ctgccagtgc cctgccagtg gacctacatc
     3181 accaccatgc cctcagcacc cagggaagac agtggcagtg cttctactga ggggcctggc
     3241 cagagcacac ctatgctact caagcggaag ccagcggcca ccatgtgcat cacgcagttt
     3301 atgaagaagc ggcgctatga tggacaggtt gggtctgggg acatggatgg cttccaggca
     3361 gacacagaag aggatgaaga ggatgacaca gactgtatga tcatagatgt gccagatgtt
     3421 gggagtgacg tgtcagaagc tcccatacct gctcccacac tttgcaagtg aggatccacc
     3481 ggatctagat aactgatcat aatcagccat accacatt
//
