LOCUS       sequence-88410-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..52
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     3..23
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             97..771
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
       61 gaaccgtcag atccgctagc gctaccggtc gccaccatgg tgagtgtgat taaaccagac
      121 atgaagatca agctgcgtat ggaaggcgct gtaaatggac acccgttcgc gattgaagga
      181 gttggccttg ggaagccttt cgagggaaaa cagagtatgg accttaaagt caaagaaggc
      241 ggacctctgc ctttcgccta tgacatcttg acaactgtgt tctgttacgg caacagggta
      301 ttcgccaaat acccagaaaa tatagtagac tatttcaagc agtcgtttcc tgagggctac
      361 tcttgggaac gaagcatgaa ttacgaagac gggggcattt gtaacgcgac aaacgacata
      421 accctggatg gtgactgtta tatctatgaa attcgatttg atggtgtgaa ctttcctgcc
      481 aatggtccag ttatgcagaa gaggactgtg aaatgggagc catccactga gaaattgtat
      541 gtgcgtgatg gagtgctgaa gggtgatgtt aacatggctc tgtcgcttga aggaggtggc
      601 cattaccgat gtgacttcaa aactacttat aaagctaaga aggttgtcca gttgccagac
      661 tatcactttg tggaccacca cattgagatt aaaagccacg acaaagatta cagtaatgtt
      721 aatctgcatg agcacgccga agcgcattct gagctgccga ggcaggccaa gtccggactc
      781 agatctaagc tgaaccctcc tgatgagagt ggccccggct gcatgagctg caagtgtgtg
      841 ctctcctgag aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagata
      901 actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
      961 acacctcccc ctgaacctga aacataaaat gaat
//
