LOCUS       sequence-88411-e        1740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1740)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..706
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     CDS             complement(1502..1513)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagt gtgattaaac cagacatgaa
       61 gatcaagctg cgtatggaag gcgctgtaaa tggacacccg ttcgcgattg aaggagttgg
      121 ccttgggaag cctttcgagg gaaaacagag tatggacctt aaagtcaaag aaggcggacc
      181 tctgcctttc gcctatgaca tcttgacaac tgtgttctgt tacggcaaca gggtattcgc
      241 caaataccca gaaaatatag tagactattt caagcagtcg tttcctgagg gctactcttg
      301 ggaacgaagc atgaattacg aagacggggg catttgtaac gcgacaaacg acataaccct
      361 ggatggtgac tgttatatct atgaaattcg atttgatggt gtgaactttc ctgccaatgg
      421 tccagttatg cagaagagga ctgtgaaatg ggagccatcc actgagaaat tgtatgtgcg
      481 tgatggagtg ctgaagggtg atgttaacat ggctctgtcg cttgaaggag gtggccatta
      541 ccgatgtgac ttcaaaacta cttataaagc taagaaggtt gtccagttgc cagactatca
      601 ctttgtggac caccacattg agattaaaag ccacgacaaa gattacagta atgttaatct
      661 gcatgagcac gccgaagcgc attctgagct gccgaggcag gccaagtccg gactcagatc
      721 tcgagccatg aagccaggat tcagtccccg tgggggtggc tttggcggcc gagggggctt
      781 tggtgaccgt ggtggtcgtg gaggccgagg gggctttggc gggggccgag gccaaggcgg
      841 aggctttaga ggtcgtggac gaggaggagg tggaggcggc ggcggcggtg gaggaggagg
      901 aagaggtggt ggaggcttcc attttggtgg caaccggggt cgtggtcggg gaggaaaaag
      961 aggaaaccag tcggggaaga atgtgatggt ggagccgcat cggcatgagg gtgtcttcat
     1021 ttgtcgggga aaggaagatg cactggtcac caagaacctg gtccctgggg aatcagttta
     1081 tggagagaag agagtctcga tttcggaagg agatgacaaa attgagtacc gagcctggaa
     1141 ccccttccgc tccaagcttg cagcagcaat cctgggtggt gtggaccaga tccacatcaa
     1201 accgggggct aaggttctct acctcggggc tgcctcgggc accacggtct cccatgtctc
     1261 tgacatcgtt ggtccggatg gtctagtcta tgcagtcgag ttctcccacc gctctggccg
     1321 tgacctcatt aacttggcca agaagaggac caacatcatt cctgtgatcg aggatgctcg
     1381 acacccacac aaataccgca tgctcatcgc aatggtggat gtgatctttg ctgatgtggc
     1441 ccagccagac cagacccgga ttgtggccct gaatgcccac accttcctgc gtaatggagg
     1501 acactttgtg atttccatta aggccaactg cattgactcc acagcctcag ccgaggccgt
     1561 gtttgcctcc gaagtgaaaa agatgcaaca ggagaacatg aagccgcagg agcagttgac
     1621 ccttgagcca tatgaaagag accatgccgt ggtcgtggga gtgtacaggc caccccccaa
     1681 ggtgaagaac tgaggatcca ccggatctag ataactgatc ataatcagcc ataccacatt
//
