LOCUS       sequence-88415-e        1200 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1200)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-H2B-6
FEATURES             Location/Qualifiers
     source          1..1200
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..456
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      469..478
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             475..1152
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgccaccat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc
      121 tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc
      181 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
      241 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
      301 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
      361 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
      421 ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtcgc caccatggtg
      481 agtgtgatta aaccagacat gaagatcaag ctgcgtatgg aaggcgctgt aaatggacac
      541 ccgttcgcga ttgaaggagt tggccttggg aagcctttcg agggaaaaca gagtatggac
      601 cttaaagtca aagaaggcgg acctctgcct ttcgcctatg acatcttgac aactgtgttc
      661 tgttacggca acagggtatt cgccaaatac ccagaaaata tagtagacta tttcaagcag
      721 tcgtttcctg agggctactc ttgggaacga agcatgaatt acgaagacgg gggcatttgt
      781 aacgcgacaa acgacataac cctggatggt gactgttata tctatgaaat tcgatttgat
      841 ggtgtgaact ttcctgccaa tggtccagtt atgcagaaga ggactgtgaa atgggagcca
      901 tccactgaga aattgtatgt gcgtgatgga gtgctgaagg gtgatgttaa catggctctg
      961 tcgcttgaag gaggtggcca ttaccgatgt gacttcaaaa ctacttataa agctaagaag
     1021 gttgtccagt tgccagacta tcactttgtg gaccaccaca ttgagattaa aagccacgac
     1081 aaagattaca gtaatgttaa tctgcatgag cacgccgaag cgcattctga gctgccgagg
     1141 caggccaagt aatgataggc ggccgcgact ctagatcata atcagccata ccacattgag
//
