LOCUS       sequence-88419-e        2085 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Note- This plasmid contains Keratin 18. Localization: Cytokeratin, 
            Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2085)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Keratin-17
FEATURES             Location/Qualifiers
     source          1..2085
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1357..1366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1363..2040
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 gacgtcagat ccgctagcac catgagcttc accactcgct ccaccttctc caccaactac
       61 cggtccctgg gctctgtcca ggcgcccagc tacggcgccc ggccggtcag cagcgcggcc
      121 agcgtctatg caggcgctgg gggctctggt tcccggatct ccgtgtcccg ctccaccagc
      181 ttcaggggcg gcatggggtc cgggggcctg gccaccggga tagccggggg tctggcagga
      241 atgggaggca tccagaacga gaaggagacc atgcaaagcc tgaacgaccg cctggcctct
      301 tacctggaca gagtgaggag cctggagacc gagaaccgga ggctggagag caaaatccgg
      361 gagcacttgg agaagaaggg accccaggtc agagactgga gccattactt caagatcatc
      421 gaggacctga gggctcagat cttcgcaaat actgtggaca atgcccgcat cgttctgcag
      481 attgacaatg cccgtcttgc tgctgatgac tttagagtca agtatgagac agagctggcc
      541 atgcgccagt ctgtggagaa cgacatccat gggctccgca aggtcattga tgacaccaat
      601 atcacacgac tgcagctgga gacagagatc gaggctctca aggaggagct gctcttcatg
      661 aagaagaacc acgaagagga agtaaaaggc ctacaagccc agattgccag ctctgggttg
      721 accgtggagg tagatgcccc caaatctcag gacctcgcca agatcatggc agacatccgg
      781 gcccaatatg acgagctggc tcggaagaac cgagaggagc tagacaagta ctggtctcag
      841 cagattgagg agagcaccac agtgctcacc acacagtctg ctgaggttgg agctgctgag
      901 acgacgctca cagagctgag acgtacagtc cagtccttgg agatcgacct ggactccatg
      961 agaaatctga aggccagctt ggagaacagc ctgagggagg tggaggcccg ctacgcccta
     1021 cagatggagc agctcaacgg gatcctgctg caccttgagt cagagctggc acagacccgg
     1081 gcagagggac agcgccaggc ccaggagtat gaggccctgc tgaacatcaa ggtcaagctg
     1141 gaggctgaga tcgccaccta ccgccgcctg ctggaagatg gcgaggactt taatcttggt
     1201 gatgccttgg acagcagcaa ctccatgcaa accatccaaa agaccaccac ccgccggata
     1261 gtggatggca aagtggtgtc tgagaccaat gacaccaaag ttctgaggca tcgaattctg
     1321 cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag tgtgattaaa
     1381 ccagacatga agatcaagct gcgtatggaa ggcgctgtaa atggacaccc gttcgcgatt
     1441 gaaggagttg gccttgggaa gcctttcgag ggaaaacaga gtatggacct taaagtcaaa
     1501 gaaggcggac ctctgccttt cgcctatgac atcttgacaa ctgtgttctg ttacggcaac
     1561 agggtattcg ccaaataccc agaaaatata gtagactatt tcaagcagtc gtttcctgag
     1621 ggctactctt gggaacgaag catgaattac gaagacgggg gcatttgtaa cgcgacaaac
     1681 gacataaccc tggatggtga ctgttatatc tatgaaattc gatttgatgg tgtgaacttt
     1741 cctgccaatg gtccagttat gcagaagagg actgtgaaat gggagccatc cactgagaaa
     1801 ttgtatgtgc gtgatggagt gctgaagggt gatgttaaca tggctctgtc gcttgaagga
     1861 ggtggccatt accgatgtga cttcaaaact acttataaag ctaagaaggt tgtccagttg
     1921 ccagactatc actttgtgga ccaccacatt gagattaaaa gccacgacaa agattacagt
     1981 aatgttaatc tgcatgagca cgccgaagcg cattctgagc tgccgaggca ggccaagtaa
     2041 tgataggcgg ccgcgactct agatcataat cagccatacc acatt
//
