LOCUS       sequence-88425-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Mito-3
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..92
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             114..791
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 acgtcagatc cgctagcatg tccgtcctga cgccgctgct gctgcggggc ttgacaggct
       61 cggcccggcg gctcccagtg ccgcgcgcca agatccattc gttgggggat ccaatggtga
      121 gtgtgattaa accagacatg aagatcaagc tgcgtatgga aggcgctgta aatggacacc
      181 cgttcgcgat tgaaggagtt ggccttggga agcctttcga gggaaaacag agtatggacc
      241 ttaaagtcaa agaaggcgga cctctgcctt tcgcctatga catcttgaca actgtgttct
      301 gttacggcaa cagggtattc gccaaatacc cagaaaatat agtagactat ttcaagcagt
      361 cgtttcctga gggctactct tgggaacgaa gcatgaatta cgaagacggg ggcatttgta
      421 acgcgacaaa cgacataacc ctggatggtg actgttatat ctatgaaatt cgatttgatg
      481 gtgtgaactt tcctgccaat ggtccagtta tgcagaagag gactgtgaaa tgggagccat
      541 ccactgagaa attgtatgtg cgtgatggag tgctgaaggg tgatgttaac atggctctgt
      601 cgcttgaagg aggtggccat taccgatgtg acttcaaaac tacttataaa gctaagaagg
      661 ttgtccagtt gccagactat cactttgtgg accaccacat tgagattaaa agccacgaca
      721 aagattacag taatgttaat ctgcatgagc acgccgaagc gcattctgag ctgccgaggc
      781 aggccaagta agcggccgcg actctagatc ataatcagcc ataccacat
//
