LOCUS       sequence-88426-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: Dronpa-Mito-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..57
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     8..28
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             86..160
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      188..197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             194..871
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
       61 ttagtgaacc gtcagatccg ctagcatgtc cgtcctgacg ccgctgctgc tgcggggctt
      121 gacaggctcg gcccggcggc tcccagtgcc gcgcgccaag atccattcgt tgggggatcc
      181 accggtcgcc accatggtga gtgtgattaa accagacatg aagatcaagc tgcgtatgga
      241 aggcgctgta aatggacacc cgttcgcgat tgaaggagtt ggccttggga agcctttcga
      301 gggaaaacag agtatggacc ttaaagtcaa agaaggcgga cctctgcctt tcgcctatga
      361 catcttgaca actgtgttct gttacggcaa cagggtattc gccaaatacc cagaaaatat
      421 agtagactat ttcaagcagt cgtttcctga gggctactct tgggaacgaa gcatgaatta
      481 cgaagacggg ggcatttgta acgcgacaaa cgacataacc ctggatggtg actgttatat
      541 ctatgaaatt cgatttgatg gtgtgaactt tcctgccaat ggtccagtta tgcagaagag
      601 gactgtgaaa tgggagccat ccactgagaa attgtatgtg cgtgatggag tgctgaaggg
      661 tgatgttaac atggctctgt cgcttgaagg aggtggccat taccgatgtg acttcaaaac
      721 tacttataaa gctaagaagg ttgtccagtt gccagactat cactttgtgg accaccacat
      781 tgagattaaa agccacgaca aagattacag taatgttaat ctgcatgagc acgccgaagc
      841 gcattctgag ctgccgaggc aggccaagta atgataggcg gccgcgactc tagatcataa
      901 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
      961 tgaacctgaa acataaaaat gaatgcaatt gtgt
//
