LOCUS       sequence-88427-e        6722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6722)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-MyosinIIA-C-18
FEATURES             Location/Qualifiers
     source          1..6722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             38..712
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 gtgaccgctc gagccgctag cgctaccggt cgccaccatg gtgagtgtga ttaaaccaga
       61 catgaagatc aagctgcgta tggaaggcgc tgtaaatgga cacccgttcg cgattgaagg
      121 agttggcctt gggaagcctt tcgagggaaa acagagtatg gaccttaaag tcaaagaagg
      181 cggacctctg cctttcgcct atgacatctt gacaactgtg ttctgttacg gcaacagggt
      241 attcgccaaa tacccagaaa atatagtaga ctatttcaag cagtcgtttc ctgagggcta
      301 ctcttgggaa cgaagcatga attacgaaga cgggggcatt tgtaacgcga caaacgacat
      361 aaccctggat ggtgactgtt atatctatga aattcgattt gatggtgtga actttcctgc
      421 caatggtcca gttatgcaga agaggactgt gaaatgggag ccatccactg agaaattgta
      481 tgtgcgtgat ggagtgctga agggtgatgt taacatggct ctgtcgcttg aaggaggtgg
      541 ccattaccga tgtgacttca aaactactta taaagctaag aaggttgtcc agttgccaga
      601 ctatcacttt gtggaccacc acattgagat taaaagccac gacaaagatt acagtaatgt
      661 taatctgcat gagcacgccg aagcgcattc tgagctgccg aggcaggcca agtccggact
      721 cagatctggc agcggtggag gcagcgcatc cggcggaagc ggaagcgctc agcaggctgc
      781 agacaagtac ctctatgtgg ataaaaactt catcaataac ccgctggccc aagctgactg
      841 ggctgccaag aagttggtat gggtgccttc cagcaagaat ggctttgaac cagccagcct
      901 caaggaggag gtgggagaag aggccattgt agagctggta gagaatggga agaaggtgaa
      961 ggtgaacaag gacgacatcc agaagatgaa cccacccaag ttctccaagg tggaggacat
     1021 ggcagagctc acgtgcctca acgaagcttc ggtgctgcac aacctcaagg agcgatacta
     1081 ctcagggctt atctacacct attcaggcct gttctgtgtg gtcatcaacc cttataagaa
     1141 cctgcccatc tactcagagg agatcgtgga gatgtacaag ggcaagaaga ggcacgagat
     1201 gccaccccac atctacgcca tcacagatac tgcctaccgg agcatgatgc aggaccggga
     1261 agatcagtcc atcctgtgca cgggggagtc tggagcaggg aagacagaga acaccaagaa
     1321 agtcatccag tacctggcac atgtggcctc ctcacacaag agcaagaagg accaggggga
     1381 gttggagcgg cagctgctac aggccaaccc tatcctagag gcctttggaa acgccaagac
     1441 ggtgaagaat gacaactcct ctcgattcgg taaattcatt cgtatcaact ttgatgtcaa
     1501 tggctacatt gttggtgcca acattgagac ttatcttctg gagaaatctc gtgctatccg
     1561 ccaagccaaa gaggagcgga ccttccacat cttctactac ctgctgtctg gggccggaga
     1621 acacctgaag actgatctcc tgttggagcc atacaacaaa taccgcttcc tgtccaacgg
     1681 gcacgtcacc atccctgggc agcaggacaa ggacatgttc caggagacaa tggaggccat
     1741 gagaattatg ggtatcccag aggatgagca gatgggcttg ctgcgggtca tctctggggt
     1801 ccttcagctt ggcaacattg ccttcaagaa ggagcggaac actgaccagg cgtccatgcc
     1861 ggacaacaca gctgctcaaa aggtgtccca cctcctgggg atcaatgtga ccgacttcac
     1921 cagaggcatc ctcaccccac gcatcaaggt gggcagagac tatgtgcaga aggcgcagac
     1981 taaagagcag gctgactttg ccattgaggc cttggccaag gctacctatg agcggatgtt
     2041 ccgctggctg gtgcttcgca tcaacaaagc tctggacaag accaagaggc agggcgcctc
     2101 atttatcggg atactggaca tcgctggctt tgaaatcttt gatctgaact ccttcgagca
     2161 gctgtgcatc aactacacca acgagaagct gcagcagctg ttcaaccaca ccatgttcat
     2221 cctggagcag gaggagtacc agcgagaggg catcgagtgg aacttcatcg acttcggcct
     2281 ggacctgcag ccctgcatcg acctcattga gaagccggcg ggtcccccag gcatcctggc
     2341 cctgctagat gaggagtgct ggtttcctaa ggccactgac aagagcttcg tggagaaggt
     2401 ggtgcaggag cagggcaccc accccaagtt ccagaagccc aagcaactga aggacaaggc
     2461 tgatttctgc attatccact atgccggcaa ggtggactat aaagctgacg agtggctgat
     2521 gaagaacatg gaccccttga acgacaacat cgccacgctg cttcaccagt cctcagacaa
     2581 gtttgtctct gagctgtgga aggatgtgga tcggatcatt ggcttggacc aagtggctgg
     2641 aatgtccgag acagcactac ctggtgcctt caagacccgg aagggcatgt tccgtactgt
     2701 cggacagctg tacaaggagc agctggccaa gctcatggcc acgttgagga ataccaaccc
     2761 caacttcgtg cgctgcatca ttcccaacca tgagaagaag gccggcaaac tggacccgca
     2821 cttggtgctg gaccagctgc gctgcaatgg cgtccttgag ggcatccgga tctgccgcca
     2881 gggctttccc aacagggtgg tcttccagga gttccggcag aggtatgaga tcctcacccc
     2941 caactccatc ccgaagggct tcatggatgg caagcaagcg tgtgtgctca tgatcaaagc
     3001 cttggagctt gacagcaacc tgtaccgcat cggccagagc aaagtgttct tccgggcagg
     3061 agtgctcgcc cacctggagg aagagcggga cctgaagatc accgatgtca tcattggctt
     3121 ccaggcctgc tgcaggggct acctggccag gaaggccttt gccaagaggc agcaacagct
     3181 gaccgccatg aaggtcctac agaggaactg tgctgcgtac ctcaggctgc gcaactggca
     3241 gtggtggagg ctcttcacca aggtcaagcc cctgttgaac tcaataagac atgaggatga
     3301 gctgttagcc aaggaggcgg aactgacaaa ggttcgagag aaacatctgg ctgcagagaa
     3361 caggctgaca gagatggaga cgatgcagtc tcagctcatg gcagagaagc tgcagcttca
     3421 ggagcagctg caggcggaga cagagctgtg tgccgaggct gaggagctcc gggcccgtct
     3481 gacagcgaag aagcaggagc tggaggagat atgccatgac ctggaggcca gggtggagga
     3541 ggaggaggag cgctgccagt acctgcaggc cgagaagaag aagatgcagc agaacatcca
     3601 ggaacttgag gagcagttgg aggaggagga gagcgcccgg cagaagctgc agcttgagaa
     3661 ggtgaccacc gaggccaagc tgaagaaact ggaggaggac cagatcatca tggaggacca
     3721 gaactgcaaa ctggccaagg agaagaaact gctggaagac agagtagctg agttcactac
     3781 caacctcatg gaagaggagg agaagtccaa gagcctggcc aagctcaaga acaagcacga
     3841 ggcaatgatc accgacctgg aagagcgcct ccgtagggag gagaagcaga ggcaggagtt
     3901 ggagaagacc cgtcgcaagc tggagggaga ctccacagac ctcagtgacc agattgctga
     3961 gctccaggcg cagatagcag agctcaagat gcagctggcc aagaaggagg aggagttgca
     4021 ggctgccttg gccagagtgg aagaagaagc tgctcagaag aatatggccc tgaagaagat
     4081 ccgagaactg gaaactcaga tatctgagct ccaggaggac ctggagtcgg agcgagcctc
     4141 caggaataaa gccgagaagc agaaacggga tctgggagag gagctggagg cgctgaagac
     4201 agagctggag gacacgctgg actccacggc tgcccagcag gagctgaggt cgaagcgtga
     4261 gcaggaggtg agcatcctga agaagactct ggaggacgag gccaagaccc atgaggccca
     4321 gatccaggag atgaggcaga agcactcaca ggctgtggag gagctggcag atcagttgga
     4381 gcagacgaag cgggtaaaag ctacccttga gaaggcgaag cagaccctgg agaatgagcg
     4441 gggagagctg gccaatgagg tgaaggccct gctgcaaggc aagggcgact cagagcacaa
     4501 gcgcaagaag gtggaggcgc agctgcaaga actgcaggtc aagttcagcg agggagagcg
     4561 cgtgcgaacc gaactggccg acaaggtcac caagctgcag gttgaactgg acagtgtgac
     4621 cggtctcctt agccagtctg acagcaagtc cagcaagctt acgaaggact tctctgcgct
     4681 ggagtcccag cttcaggaca cacaggagtt gctccaggag gagaaccggc agaagctgag
     4741 cctgagcacc aagctcaagc agatggagga tgagaaaaac tccttcaggg agcagctgga
     4801 ggaggaggag gaggccaagc gcaacttgga gaagcagatc gccacgctcc atgcccaggt
     4861 gaccgacatg aagaagaaga tggaggacgg tgtagggtgc ctggagactg cagaggaggc
     4921 gaagcggagg cttcagaagg acttggaagg cctgagccag cggcttgagg agaaggtggc
     4981 tgcctacgat aagctggaga agaccaagac acggctgcag caggagctgg acgacctgct
     5041 ggttgacctg gaccaccagc ggcagagcgt ctccaacctg gaaaagaagc agaagaagtt
     5101 cgaccagctc ctagccgagg agaagaccat ctcggccaag tatgcagagg agcgtgaccg
     5161 agctgaggct gaggcccgtg agaaggagac aaaggcgcta tcactggccc gggcgcttga
     5221 ggaggccatg gagcagaagg cagagctgga gcggctcaac aagcagttcc gcacggagat
     5281 ggaggacctc atgagctcca aggatgacgt gggcaagagt gtccacgagc tggagaagtc
     5341 caagcgggcc ttggagcagc aggtggagga gatgaagacc cagctggagg agctggagga
     5401 tgagctgcag gccacggagg atgccaagct ccgcctggag gtgaacctgc aggccatgaa
     5461 ggcccagttt gagcgggatc tgcagggccg ggatgaacag agcgaggaga agaagaagca
     5521 gctggtcaga caggtgcggg agatggaggc ggagctggag gatgagagga agcagcgctc
     5581 catggccatg gccgcacgca agaaactgga gatggatctg aaggacctgg aggcacacat
     5641 tgacacagcc aataagaacc gggaagaggc catcaaacag ctgcggaagc ttcaggccca
     5701 gatgaaggac tgcatgcggg agctggacga cacgcgcgcc tcccgggagg agatcctggc
     5761 gcaggccaag gagaatgaga agaagctgaa gagcatggag gccgagatga ttcagctgca
     5821 ggaggaactg gcagctgctg agcgtgctaa gcgtcaggcc caacaggaac gggacgagct
     5881 ggctgatgag atcgccaaca gcagtggcaa aggggcccta gcattagagg agaagcggcg
     5941 actggaggcc cgcattgccc tgctggagga ggagctggag gaggaacagg gcaacacgga
     6001 gctgatcaac gatcggctga agaaggccaa cctgcagatc gaccaaataa acaccgacct
     6061 gaacctggaa cgcagccacg cacagaagaa tgagaatgcg cgacagcagc tggaacgcca
     6121 gaacaaggag ctcaaggcca agctgcagga aatggagagt gctgtcaagt ccaaatacaa
     6181 ggcctccatc gcggccttgg aggccaaaat tgcacagctg gaggaacagc tggacaacga
     6241 gaccaaggag cgccaggcag cctccaagca ggtgcgccgg acggagaaga agctgaagga
     6301 cgtgctgctg caggtggagg acgagcggag gaacgcggaa cagttcaagg accaggctga
     6361 caaggcgtcc acccgcctga agcagcttaa acggcagcta gaggaggctg aagaggaggc
     6421 ccagcgggcc aatgcctcac gccggaagct gcagcgtgag ctggaagatg ccacagagac
     6481 cgctgatgct atgaaccgcg aggtcagctc cctgaagaac aaactgaggc gtggggacct
     6541 gccatttgtc gtgactcgcc gaattgttcg gaaaggcact ggcgactgct cagacgagga
     6601 ggtcgacggt aaagcagatg gggccgatgc caaggcagct gaataggaat tctgcagtcg
     6661 acggtaccgc gggcccggga tccaccggat ctagataact gatcataatc agccatacca
     6721 ca
//
