LOCUS       sequence-88428-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Nucleus-9
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..107
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     58..78
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      139..148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             145..819
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     CDS             850..870
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             874..894
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             898..918
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
       61 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
      121 gtcagatccg ctagcattgc caccatggtg agtgtgatta aaccagacat gaagatcaag
      181 ctgcgtatgg aaggcgctgt aaatggacac ccgttcgcga ttgaaggagt tggccttggg
      241 aagcctttcg agggaaaaca gagtatggac cttaaagtca aagaaggcgg acctctgcct
      301 ttcgcctatg acatcttgac aactgtgttc tgttacggca acagggtatt cgccaaatac
      361 ccagaaaata tagtagacta tttcaagcag tcgtttcctg agggctactc ttgggaacga
      421 agcatgaatt acgaagacgg gggcatttgt aacgcgacaa acgacataac cctggatggt
      481 gactgttata tctatgaaat tcgatttgat ggtgtgaact ttcctgccaa tggtccagtt
      541 atgcagaaga ggactgtgaa atgggagcca tccactgaga aattgtatgt gcgtgatgga
      601 gtgctgaagg gtgatgttaa catggctctg tcgcttgaag gaggtggcca ttaccgatgt
      661 gacttcaaaa ctacttataa agctaagaag gttgtccagt tgccagacta tcactttgtg
      721 gaccaccaca ttgagattaa aagccacgac aaagattaca gtaatgttaa tctgcatgag
      781 cacgccgaag cgcattctga gctgccgagg caggccaagg gatcctccgg actcagatct
      841 cgagctgatc caaaaaagaa gagaaaggta gatccaaaaa agaagagaaa ggtagatcca
      901 aaaaagaaga gaaaggtaaa gcttgaataa gcggccgcga ctctagatca taatcagcca
      961 taccacattt gtagaggttt tacttgcttt aaaaa
//
