LOCUS       sequence-8843-ex       13173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13173)
  AUTHORS   Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, 
            Lichtman JW
  TITLE     Transgenic strategies for combinatorial expression of fluorescent 
            proteins in the nervous system.
  JOURNAL   Nature. 2007 Nov 1. 450(7166):56-62.
  PUBMED    17972876
REFERENCE   2  (bases 1 to 13173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Nov 1. 450(7166):56-62."
COMMENT     Sequence Label: Thy1-Brainbow-1.0 H
FEATURES             Location/Qualifiers
     source          1..13173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(6254..6287)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(6304..6337)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6353..6362
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6357..6366
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6359..7036
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     CDS             7079..7753
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     primer_bind     complement(7810..7830)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7810..7828)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7848..7865)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7854..7965
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(8124..8157)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      8276..8285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8282..8284,8285..8287,8288..9001)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    9124..9245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9161..9180)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9215..9234
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(9260..9293)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      9416..9425
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9422..9424,9425..9427,9428..10141)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    10184..10217
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(13004..13023)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13123..13145
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcaggatc gcggccgcga tccccgggcg agctcgaatt cagagaccgg
     2101 gaaccaaact agcctttaaa aaacataagt acaggagcca gcaagatggc tcagtgggta
     2161 aaggtgccta ccagcaagcc tgacagcctg agttcagtcc ccacgaacta cgtggtagga
     2221 gaggaccaac caactctgga aatctgttct gcaaacacat gctcacacac acacacacaa
     2281 atagtataaa caattttaaa tttcatttaa aaataatttg taaacaaaat cattagcaca
     2341 ggttttagaa agagcctctt ggtgacatca agttgatgct gtagatgggg tatcattcct
     2401 gaggacccaa aaccgggtct cagcctttcc ccattctgag agttctctct tttctcagcc
     2461 actagctgaa gagtagagtg gctcagcact gggctcttga gttcccaagt cctacaactg
     2521 gtcagcctga ctactaacca gccatgaaga aacaaggagt ggatgggctg agtctgctgg
     2581 gatgggagtg gagttagtaa gtggccatgg atgtaatgac cccagcaatg ctggctagaa
     2641 ggcatgcctc ctttccttgt ctggagacgg aacgggatca tcttgtactc acagaaggga
     2701 gaacattcta gctggttggg ccaaaatgtg caagttcacc tggaggtggt ggtgcatgct
     2761 tttaactcca gtactcagga ggcagggcca ggtggatctc tgtgagttca agaccagcct
     2821 gcactatgga gagagttttg ggacagccag agttacacag aaaaatcctg gtggaaaatc
     2881 tgaaagaaag agagaaagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa
     2941 agaaagaaag aaagaaagga agaaagaaag aaagagtggc aggcaggcag gcaggaggaa
     3001 ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa
     3061 aataggtgcg acttcaagat ccggagttac aagcagaatg cactgtttcc ctaacagggc
     3121 caagtgtttt gagtaactga aggtgggcat gatgcctggg aagcagaaac aagccaggca
     3181 gatgcacccc ttgccttgct tccgaagggc tgcagtagca tggaaaacat ggaaaacaac
     3241 caatccattc cctttgctga tataacaggc tccaaagcca aaacctgtca ctggaggctc
     3301 aagagcagat ctccagccaa gaggcaaagg aatgggggaa gctggagggc ctccctctgg
     3361 ttatccaggc ttctgaaggt tcaagcaaag aaagggttac aaccttaaaa ggagagcgtc
     3421 ccggggtatg ggtagaagac tgctccaccc cgacccccag ggtccctaac cgtcttttcc
     3481 ctgggcgagt cagcccaatc acaggactga gagtgcctct ttagtagcag caagccactt
     3541 cggacaccca aatggaacac ctccagtcag ccctcgccga ccaccccacc ccctccatcc
     3601 ttttccctca gcctccgatt ggctgaatct agagtccctc cctgctcccc cctctctccc
     3661 cacccctggt aaaactgcgg gcttcaggct gggtgcagca actggaggcg ttggcgacca
     3721 ggaggaggct gcagctaggg gagtccaggt gagagcaggc cgacgggagg gacccgcaca
     3781 tgcaaggacc gccgcagggc gaggatgcaa gccttcccca gctacagttt tgggaaagga
     3841 taccagggcg ctcctatatg ggggcgcggg aacctgggga aagaaggtgc tcccaggtcg
     3901 aggtgggaga ggaaggcagt gcggggtcac gggctttctc cctgctaacg gacgctttcg
     3961 aagagtgggt gccggagaga accatgagga aggacatcaa ggacagcctt tggtccccaa
     4021 gctcaaatcg ctttagtggt gcgaatagag ggaggaggtg ggtggcaaac tggagggagt
     4081 ccccagcggt gacctcgtgg ctggctggtg cggggcacca ggtaagaaac gcaatgttgc
     4141 gggaggggac tgggtggcag gcgcggggga ggggaaagct agaaaggatg cgagggagcg
     4201 gaggggggag ggagcggggg aatctcaact ggtagaggaa agttaaaatg aggaaatagc
     4261 atcagggtgg ggttagccaa gccgggcctc agggaaaggg cgcaaagttt gtctgggtgt
     4321 gggcttaggt gggctgggta tgagattcgg ggcgccgaaa acactgctgc gcctctgcca
     4381 aatcacgcta cccctgtatc tagttctgct aggcttctcc agccccagcc ccaattcttt
     4441 tctcagtgtc cccttccctc ccctgaatct caagcccaca ctccctcctc cataacccac
     4501 tgttatcaaa tctaagtcat ttgccaccca acaaccatca ggaggcggaa gcagacggga
     4561 ggagtttgag atcaacttgg gctacatcac gagttccaag ctcaccaagg cttcttaagg
     4621 agaccttgtc tctaaaatta attaattaat taattaatag tcccctttct ctgccacaga
     4681 accttgggat ctggctcctg gtcgcagctc cccccacccc aggctgacat tcactgccat
     4741 agcccatccg gaaatcctag tctattcccc atggatcttg aactgcagag agaatggcag
     4801 agtggcccgc cctgtgcaaa ggatgttcct agcctaggtg gagctcgcga actcggagac
     4861 tgtgcctctc ttgggcaagg acaggctaga cagcctgccg gtgttgagct agggcactgt
     4921 ggggaaggca gagaacctgt gcagggcacg caatgaacac aggaccagaa aactgcagcc
     4981 ctaggaacac tcaagagctg gccatttgca agcatctctg gcctccgtgc ttctcactca
     5041 tgtcccatgt cttatacagg cctctgtggc acctcgcttg cctgatctca tccctagccg
     5101 ttaagctttc tgcatgactt atcacttggg gcataatgct ggatacctac cattttctta
     5161 gaccccatca aaatcctatt tgagtgtacg gttcggagaa ctccttattt atccggtaaa
     5221 tgtcttttac tctgctctca gggagctgag gcaggacatt cctgagatac attgggagag
     5281 gaatacagtt tcaataaaat aataggttgg gtggaggtac atgcctataa tgccaccact
     5341 caggaaatgg tggcagcttc gtgagtttga ggccaaccca agaaacatag tgaaaccctg
     5401 tcagtaaata agtaagcaag tatttgagta tctactatat gctagggctg acctggacat
     5461 taggggtcat cttctgaaca aactagtgct tgagggaggt atttggggtt tttgtttgtt
     5521 taatggatct gaatgagttc cagagactgg ctacacagcg atatgactga gcttaacacc
     5581 cctaaagcat acagtcagac caattagaca ataaaaggta tgtatagctt accaaataaa
     5641 aaaattgtat tttcaagaga gtgtctgtct gtgtagccct ggctgttctt gaactcactc
     5701 tgtagaccag gctggcctgg aaatccatct gcctctgcct ctctgcctct ctgcctctct
     5761 gcctctctct ctgcctctct ctgcctctct ctgcccctct ctgcccctct ctgcccctct
     5821 ctgccgccct ctgccttttg ccctctgccc tctgttctct ggcctctgcc ctctccctgc
     5881 cttctccctc tgcctctgcc tcttgagtgc tggaatcaaa ggtgtgagct ctataggtct
     5941 taagttccag aagaaacgta atgaagtcac ccagcaggag gtgctcaggg acagcaagac
     6001 acacacaccc aggacactag gctcccactt ccttggcttt ctctgagtgg caaaggacct
     6061 taggcagtgt cactccctaa gagaagggga taaagagagg ggctgaggta ttcagtcatg
     6121 tgctccgtgg atctcaagcc ctcaaggtaa atggggaccc acctgtccta ccagctggct
     6181 gacctgtagc tttccccacc acagaatcca agtcggaact cttggcacct agaggatctc
     6241 gactagcgag ctcataactt cgtataggat actttatacg aagttataac gttagatctc
     6301 gagataactt cgtatagcat acattatacg aagttatacc ggctagtgat tcgccaccat
     6361 ggtggcctcc tccgaggacg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg
     6421 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     6481 cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat
     6541 cctgtccccc cagttccagt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc
     6601 cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     6661 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta
     6721 caaggtgaag ttccgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac
     6781 catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga
     6841 gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat
     6901 ctacatggcc aagaagcccg tgcagctgcc cggctactac tacgtggaca ccaagctgga
     6961 catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg
     7021 ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct ccggcaccgc
     7081 ctcctccgag gacgtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccgt
     7141 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     7201 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     7261 cccccagttc cagtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgacta
     7321 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     7381 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
     7441 gaagttccgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
     7501 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
     7561 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat
     7621 ggccaagaag cccgtgcagc tgcccggcta ctactacgtg gacaccaagc tggacatcac
     7681 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
     7741 cctgttcctg taggaattgg ccgcgactct aggattgatc tagagtcgcg gctagataat
     7801 agggtaccac ctttagtgag ggttaattgc tagagctcgc tgatcagcct cgactgtgcc
     7861 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     7921 tgccactctc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     7981 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     8041 caatagcagg catgctgggg cgccgctccg agggccgcca ccacctgttc ctgtaggaat
     8101 tggccgcgac tctaggatct aggataactt cgtataggat actttatacg aagttattga
     8161 tcaattcaca gccaccatgc tgtgctgcat cagaagaact aaaccggttg agaagaatga
     8221 agaggccgat caggagctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac
     8281 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     8341 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     8401 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     8461 cctcgtgacc accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa
     8521 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     8581 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     8641 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     8701 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     8761 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     8821 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     8881 ctacctgagc taccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     8941 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     9001 aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
     9061 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     9121 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9181 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9241 tcttaacgcg cgattcgaga taacttcgta tagcatacat tatacgaagt tataccggaa
     9301 ttaattcaca gccaccatgc tgtgctgcat cagaagaact aaaccggttg agaagaatga
     9361 agaggccgat caggagctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac
     9421 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     9481 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     9541 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     9601 cctcgtgacc accctgacct ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa
     9661 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     9721 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     9781 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     9841 caagctggag tacaacgcca tcagcgacaa cgtctatatc accgccgaca agcagaagaa
     9901 cggcatcaag gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     9961 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
    10021 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
    10081 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
    10141 aagcggccgc gactctagat caccgcgact ctagatcacg cgcgaagttc ctattctcta
    10201 gaaagtatag gaacttctcg agttcgaggt ccttcctctg cagaggtctt gcttctcccg
    10261 gtcagctgac tccctcccca agtccttcaa atatctcaga acatggggag aaacggggac
    10321 cttgtccctc ctaaggaacc ccagtgctgc atgccatcat cccccccacc ctcgccccca
    10381 cccccgccac ttctccctcc atgcatacca ctagctgtca ttttgtactc tgtatttatt
    10441 ctagggctgc ttctgattat ttagtttgtt ctttccctgg agacctgtta gaacataagg
    10501 gcgtatggtg ggtaggggag gcaggatatc agtcccctgg ggcgagttcc tccctgccaa
    10561 ccaagccaga tgcctgaaag agatatggat gagggaagtt ggactgtgcc tgtacctggt
    10621 acagtcatac tctgttgaaa gaatcatcgg ggaggggggg gggctcaaga ggggagagct
    10681 ctctgagcct ttgtggacca tccaatgagg atgagggctt agattctacc aggtcattct
    10741 cagccaccac acacaagcgc tctgccatca ctgaagaagc cccctagggc tcttgggcca
    10801 gggcacactc agtaaagatg caggttcagt cagggaatga tggggaaagg ggtaggaggt
    10861 gggggaggga tcaccccctc ctctaaaaca cgagcctgct gtctccaaag gcctctgcct
    10921 gtagtgaggg tggcagaaga agacaaggag ccagaactct gactccagga tctaagtccg
    10981 tgcaggaagg ggatcctaga accatctggt tggacccagc ttaccaaggg agagccttta
    11041 ttccttcttt cccttgcccc tctgtgccag cccctcttgc tgtccctgat cccccagaca
    11101 gcgagagtct tgcaacctgc ctcttccaag acctcctaat ctcaggggca ggcggtggag
    11161 tgagatccgg cgtgcacact ttttggaaga tagctttccc aaggatcctc tcccccactg
    11221 gcagctctgc ctgtcccatc accatgtata ataccaccac tgctacagca tctcaccgag
    11281 gaaagaaaac tgcacaataa aaccaagcct ctggagtgtg tcctggtgtc tgtctcttct
    11341 gtgtcctggc gtctgtctct tctgtgttct tccaaggtca gaaacaaaaa ccacacactt
    11401 caacctggat ggctcggctg agcacttctg tgtgcagaag gtccaaccag actctggggt
    11461 accccggccc tccctattcc cttgcctcct gtctcccgct ttttatagct ccctatgctg
    11521 ggcttctctg gagagtgaaa tctttgccca aatcaatgcg cattctctct gctgagtcat
    11581 ctggcgacag cagttgagtt cacccgccaa cacatgggcc cagctatgta gccgaaccct
    11641 ggctctggaa gtgccaggga ctttgtgcat aagtatgtac catgcccttt tttcacagtc
    11701 ctagctctgc agaagtgcag cctgaaggcc tgtctgctga gaggacatgc cctggagccc
    11761 tgaaacaggc acagtgggag gaggaacgga ggatgacagg catcaggccc tcagtccaaa
    11821 agcaaccact tgagaatggg ctggagtacg aaacatgggg tcccgtccct ggatccctcc
    11881 tcaaagagta ataagtaaaa tataaacagg taccccaggc cgttctgggt ttgggttgta
    11941 atgggatcca tttgcagaga actattgaga cagcccagcc gtactgtgac aggcaatgtg
    12001 ggggaggagg ttgaatcact tggtatttag catgaataga ataattccct gaacattttt
    12061 cttaaacatc catatctaaa ttaccaccac tcgctcccag tcttcctgcc tttgcgccag
    12121 cctcctgtct ggccatgcct gaagaaggct ggagaagcca cccacctcag gccatgacac
    12181 tgccagccac ttggcaggtg cagccaaacc tgagctgtcc cagaaaggga cattctcaag
    12241 acccaggcac cctgatcagc actgacttgg agctacaagt gtcatgccag aaaagtctct
    12301 aagaaaacct tttcagggaa aagggggtga ctcaacaccg ggcaagtttg ggaagcccca
    12361 cccttcgagt gatggaagag cagataggaa gcctcagaag agagacaccg gcacccaggt
    12421 aacgttcctc atgtggtctc tgtcacacta ggtgctcttc cctggacatc tccgtgacca
    12481 cactctcagt tcttagggag atgcgggtgc tctctgaggc tatctcagag ttgcagattc
    12541 tgaggcctag agtgactaca gtcagcctag gaagccacag aggactgtgg accaggaggg
    12601 cagaagagga gaagggaaga aaaaccatca gataggactt gcaatgaaac taacccaaga
    12661 caatcataat gcagacagga atgttaaagg cgttcagcag ctggccatga cacccatctg
    12721 tccctctggc caagtcagca agcctggaag acctgggact cctgcccata tgtcctaagc
    12781 tccccaccca cccactcgtt cactgtcctt attctctctc taccttcagc cacttagttt
    12841 cctaccttaa gtcctagaat tgatcctggc gtaatagcga agaggcccgc accgatcgcc
    12901 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
    12961 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
    13021 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
    13081 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
    13141 agaggttttc accgtcatca ccgaaacgcg cga
//
