LOCUS       sequence-88431-e        2959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Scaffold, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2959)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-PSD95-N-14
FEATURES             Location/Qualifiers
     source          1..2959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2238..2247
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2244..2921
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 cgctaccgga ctcagatctc gaggccacca tggactgtct ctgtatagtg acaaccaaga
       61 aataccgcta ccaagatgaa gacacgcccc ctctggaaca cagcccggcc cacctcccca
      121 accaggccaa ttctccccct gtgattgtca acacggacac cctagaagcc ccaggatatg
      181 agttgcaggt gaatggaaca gagggggaga tggagtatga ggagatcaca ttggaaaggg
      241 gtaactcagg tctgggcttc agcatcgcag gtggcactga caacccgcac atcggtgacg
      301 acccgtccat ttttatcacc aagatcattc ctggtggggc tgcagcccag gatggccgcc
      361 tcagggtcaa tgacagcatc ctgtttgtaa atgaagtgga tgttcgggag gtgacccatt
      421 cagctgcggt ggaggccctc aaagaggcag gttccatcgt tcgcctctat gtcatgcgcc
      481 ggaaaccccc agccgaaaag gtcatggaga tcaaactcat caaagggcct aaaggacttg
      541 gcttcagcat tgcggggggc gttgggaacc agcacatccc tggagataac agcatctatg
      601 taacgaagat catcgaagga ggtgctgccc acaaggatgg caggttgcag attggagaca
      661 agatcctggc ggtcaacagt gtggggctgg aggacgtcat gcacgaggat gccgtggcag
      721 ccctgaagaa cacatatgac gttgtgtacc taaaggtggc caagcccagc aatgcctacc
      781 tgagtgacag ctatgctccc ccagacatca caacctcgta ttctcagcac ctggacaatg
      841 agatcagtca tagcagctac ttgggcactg actaccccac agccatgacc cccacttccc
      901 ctcggcgcta ctcccctgtg gccaaggacc tgctggggga ggaagacatt ccccgggaac
      961 caaggcggat cgtgatccat cggggctcca ccggcctggg cttcaacatc gtgggcggcg
     1021 aggatggtga aggcatcttc atctccttca tccttgctgg gggtccagcc gacctcagtg
     1081 gggagctacg gaagggggac cagatcctgt cggtcaatgg tgttgacctc cgcaatgcca
     1141 gtcacgaaca ggctgccatt gccctgaaga atgcgggtca gacggtcacg atcatcgctc
     1201 agtataaacc agaagagtat agtcgattcg aggccaagat ccatgatctt cgggaacagc
     1261 tcatgaatag tagcctaggc tcagggactg catccttgcg aagcaacccc aagaggggct
     1321 tctacattag ggccctgttt gattacgaca agaccaagga ctgcggtttc ttgagccagg
     1381 ccctgagctt ccgcttcggg gatgtgcttc atgtcattga cgctggtgac gaagagtggt
     1441 ggcaagcacg gcgggtccac tccgacagtg agaccgacga cattggcttc attcccagca
     1501 aacggcgggt cgagcgacga gagtggtcaa ggttaaaggc caaggactgg ggctccagct
     1561 ctggatcaca gggtcgagaa gactcggttc tgagctatga gacggtgacc cagatggaag
     1621 tgcactatgc tcgtcccatc atcatccttg gacccaccaa agaccgtgcc aacgatgatc
     1681 ttctctccga gttccccgac aagtttggat cctgtgtccc tcatacgaca cgtcctaagc
     1741 gggaatatga gatagacggc cgggattacc actttgtctc ctcccgggag aaaatggaga
     1801 aggacatcca ggcacacaag ttcattgagg ctggccagta caacagccac ctctatggga
     1861 ccagcgtcca gtctgtgcga gaggtagcag agcaggggaa gcactgcatc ctcgatgtct
     1921 cggccaatgc cgtgcggcgg ctgcaggcgg cccacctgca ccccatcgcc atcttcatcc
     1981 gtccccgctc cctggagaat gtgctagaga tcaataagcg gatcacagag gagcaagccc
     2041 ggaaagcctt cgacagagcc acgaagctgg agcaggagtt cacagagtgc ttctcagcca
     2101 tcgtagaggg cgacagcttt gaagagatct atcacaaagt gaaacgtgtc attgaagacc
     2161 tctcaggccc ctacatctgg gtcccagccc gagagagact cggcagcgca tccggcggaa
     2221 gcggaagcgg accggtcgcc accatggtga gtgtgattaa accagacatg aagatcaagc
     2281 tgcgtatgga aggcgctgta aatggacacc cgttcgcgat tgaaggagtt ggccttggga
     2341 agcctttcga gggaaaacag agtatggacc ttaaagtcaa agaaggcgga cctctgcctt
     2401 tcgcctatga catcttgaca actgtgttct gttacggcaa cagggtattc gccaaatacc
     2461 cagaaaatat agtagactat ttcaagcagt cgtttcctga gggctactct tgggaacgaa
     2521 gcatgaatta cgaagacggg ggcatttgta acgcgacaaa cgacataacc ctggatggtg
     2581 actgttatat ctatgaaatt cgatttgatg gtgtgaactt tcctgccaat ggtccagtta
     2641 tgcagaagag gactgtgaaa tgggagccat ccactgagaa attgtatgtg cgtgatggag
     2701 tgctgaaggg tgatgttaac atggctctgt cgcttgaagg aggtggccat taccgatgtg
     2761 acttcaaaac tacttataaa gctaagaagg ttgtccagtt gccagactat cactttgtgg
     2821 accaccacat tgagattaaa agccacgaca aagattacag taatgttaat ctgcatgagc
     2881 acgccgaagc gcattctgag ctgccgaggc aggccaagta atgataggcg gccgcgactc
     2941 tagatcataa tcagccata
//
