LOCUS       sequence-88432-e        1431 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1431)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Rab4a-7
FEATURES             Location/Qualifiers
     source          1..1431
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..709
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agtgtgatta aaccagacat
       61 gaagatcaag ctgcgtatgg aaggcgctgt aaatggacac ccgttcgcga ttgaaggagt
      121 tggccttggg aagcctttcg agggaaaaca gagtatggac cttaaagtca aagaaggcgg
      181 acctctgcct ttcgcctatg acatcttgac aactgtgttc tgttacggca acagggtatt
      241 cgccaaatac ccagaaaata tagtagacta tttcaagcag tcgtttcctg agggctactc
      301 ttgggaacga agcatgaatt acgaagacgg gggcatttgt aacgcgacaa acgacataac
      361 cctggatggt gactgttata tctatgaaat tcgatttgat ggtgtgaact ttcctgccaa
      421 tggtccagtt atgcagaaga ggactgtgaa atgggagcca tccactgaga aattgtatgt
      481 gcgtgatgga gtgctgaagg gtgatgttaa catggctctg tcgcttgaag gaggtggcca
      541 ttaccgatgt gacttcaaaa ctacttataa agctaagaag gttgtccagt tgccagacta
      601 tcactttgtg gaccaccaca ttgagattaa aagccacgac aaagattaca gtaatgttaa
      661 tctgcatgag cacgccgaag cgcattctga gctgccgagg caggccaagt ccggactcag
      721 atctcgagcc atgtcgcaga cggccatgtc cgaaacctac gattttttgt ttaagttctt
      781 ggttattgga aatgcaggaa ctggcaaatc ttgcttactt catcagttta ttgaaaaaaa
      841 attcaaagat gactcaaatc atacaatagg agtggaattt ggttcaaaga taataaatgt
      901 tggtggtaaa tatgtaaagt tacaaatatg ggatacagca ggacaagaac gattcaggtc
      961 cgtgacgaga agttattacc gaggcgcggc cggggctctc ctcgtctatg atatcaccag
     1021 ccgagaaacc tacaatgcgc ttactaattg gttaacagat gcccgaatgc tggcgagcca
     1081 gaacattgtg atcatccttt gtggaaacaa gaaggacctg gatgcagatc gtgaagttac
     1141 cttcttagaa gcctccagat ttgctcaaga aaatgagctg atgtttttgg aaacaagtgc
     1201 gctcacaggg gagaatgtag aagaggcttt tgtacagtgt gcaagaaaaa tacttaacaa
     1261 aatcgaatca ggtgagctgg acccagaaag aatgggctca ggtattcagt acggagatgc
     1321 tgccttgaga cagctgaggt caccgcggcg cgcacaggcc ccgaacgctc aggagtgtgg
     1381 ttgttaggga tccaccggat ctagataact gatcataatc agccatacca c
//
