LOCUS       sequence-88433-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi TGN, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-SiT-N-15
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      214..223
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             220..897
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 ccgctagcgc taccggactc agatctcgag ctcgccacca tgattcacac caacctgaag
       61 aaaaagttca gctgctgcgt cctggtcttt cttctgtttg cagtcatctg tgtgtggaag
      121 gaaaagaaga aagggagtta ctatgattcc tttaaattgc aaaccaagga attcctgcag
      181 tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagtgt gattaaacca
      241 gacatgaaga tcaagctgcg tatggaaggc gctgtaaatg gacacccgtt cgcgattgaa
      301 ggagttggcc ttgggaagcc tttcgaggga aaacagagta tggaccttaa agtcaaagaa
      361 ggcggacctc tgcctttcgc ctatgacatc ttgacaactg tgttctgtta cggcaacagg
      421 gtattcgcca aatacccaga aaatatagta gactatttca agcagtcgtt tcctgagggc
      481 tactcttggg aacgaagcat gaattacgaa gacgggggca tttgtaacgc gacaaacgac
      541 ataaccctgg atggtgactg ttatatctat gaaattcgat ttgatggtgt gaactttcct
      601 gccaatggtc cagttatgca gaagaggact gtgaaatggg agccatccac tgagaaattg
      661 tatgtgcgtg atggagtgct gaagggtgat gttaacatgg ctctgtcgct tgaaggaggt
      721 ggccattacc gatgtgactt caaaactact tataaagcta agaaggttgt ccagttgcca
      781 gactatcact ttgtggacca ccacattgag attaaaagcc acgacaaaga ttacagtaat
      841 gttaatctgc atgagcacgc cgaagcgcat tctgagctgc cgaggcaggc caagtaatga
      901 taggcggccg cgactctaga tcataatcag ccataccaca tt
//
