LOCUS       sequence-88439-e        2130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2130)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..710
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 cgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagtgtgatt aaaccagaca
       61 tgaagatcaa gctgcgtatg gaaggcgctg taaatggaca cccgttcgcg attgaaggag
      121 ttggccttgg gaagcctttc gagggaaaac agagtatgga ccttaaagtc aaagaaggcg
      181 gacctctgcc tttcgcctat gacatcttga caactgtgtt ctgttacggc aacagggtat
      241 tcgccaaata cccagaaaat atagtagact atttcaagca gtcgtttcct gagggctact
      301 cttgggaacg aagcatgaat tacgaagacg ggggcatttg taacgcgaca aacgacataa
      361 ccctggatgg tgactgttat atctatgaaa ttcgatttga tggtgtgaac tttcctgcca
      421 atggtccagt tatgcagaag aggactgtga aatgggagcc atccactgag aaattgtatg
      481 tgcgtgatgg agtgctgaag ggtgatgtta acatggctct gtcgcttgaa ggaggtggcc
      541 attaccgatg tgacttcaaa actacttata aagctaagaa ggttgtccag ttgccagact
      601 atcactttgt ggaccaccac attgagatta aaagccacga caaagattac agtaatgtta
      661 atctgcatga gcacgccgaa gcgcattctg agctgccgag gcaggccaag tccggactca
      721 gatctcgagt gcgtgagtgc atctccatcc acgttggcca ggctggtgtc cagattggca
      781 atgcctgctg ggagctctac tgcctggaac acggcatcca gcccgatggc cagatgccaa
      841 gtgacaagac cattggggga ggagatgact ccttcaacac cttcttcagt gagacgggcg
      901 ctggcaagca cgtgccccgg gctgtgtttg tagacttgga acccacagtc attgatgaag
      961 ttcgcactgg cacctaccgc cagctcttcc accctgagca gctcatcaca ggcaaggaag
     1021 atgctgccaa taactatgcc cgagggcact acaccattgg caaggagatc attgaccttg
     1081 tgttggaccg aattcgcaag ctggctgacc agtgcaccgg tcttcagggc ttcttggttt
     1141 tccacagctt tggtggggga actggttctg ggttcacctc cctgctcatg gaacgtctct
     1201 cagttgatta tggcaagaag tccaagctgg agttctccat ttacccagca ccccaggttt
     1261 ccacagctgt agttgagccc tacaactcca tcctcaccac ccacaccacc ctggagcact
     1321 ctgattgtgc cttcatggta gacaatgagg ccatctatga catctgtcgt agaaacctcg
     1381 atatcgagcg cccaacctac actaacctta accgccttat tagccagatt gtgtcctcca
     1441 tcactgcttc cctgagattt gatggagccc tgaatgttga cctgacagaa ttccagacca
     1501 acctggtgcc ctacccccgc atccacttcc ctctggccac atatgcccct gtcatctctg
     1561 ctgagaaagc ctaccatgaa cagctttctg tagcagagat caccaatgct tgctttgagc
     1621 cagccaacca gatggtgaaa tgtgaccctc gccatggtaa atacatggct tgctgcctgt
     1681 tgtaccgtgg tgacgtggtt cccaaagatg tcaatgctgc cattgccacc atcaaaacca
     1741 agcgcagcat ccagtttgtg gattggtgcc ccactggctt caaggttggc atcaactacc
     1801 agcctcccac tgtggtgcct ggtggagacc tggccaaggt acagagagct gtgtgcatgc
     1861 tgagcaacac cacagccatt gctgaggcct gggctcgcct ggaccacaag tttgacctga
     1921 tgtatgccaa gcgtgccttt gttcactggt acgtgggtga ggggatggag gaaggcgagt
     1981 tttcagaggc ccgtgaagat atggctgccc ttgagaagga ttatgaggag gttggtgtgg
     2041 attctgttga aggagagggt gaggaagaag gagaggaata ctaaggatcc accggatcta
     2101 gataactgat cataatcagc cataccacat
//
