LOCUS       sequence-8844-ex       12389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, 
            Lichtman JW
  TITLE     Transgenic strategies for combinatorial expression of fluorescent 
            proteins in the nervous system.
  JOURNAL   Nature. 2007 Nov 1. 450(7166):56-62.
  PUBMED    17972876
REFERENCE   2  (bases 1 to 12389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Nov 1. 450(7166):56-62."
COMMENT     Sequence Label: Thy1-Brainbow-1.0 L
FEATURES             Location/Qualifiers
     source          1..12389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(6254..6287)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(6296..6329)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6342..6351
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6348..7049
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(6481..6499)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6749..6768
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6943..6966
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    7179..7300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7383..7416)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      7430..7439
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7436..7438,7439..7441,7442..8155)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    8278..8399
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(8406..8439)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      8447..8456
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8453..8455,8456..8458,8459..9172)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    9295..9416
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12220..12239)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12339..12361
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcaggatc gcggccgcga tccccgggcg agctcgaatt cagagaccgg
     2101 gaaccaaact agcctttaaa aaacataagt acaggagcca gcaagatggc tcagtgggta
     2161 aaggtgccta ccagcaagcc tgacagcctg agttcagtcc ccacgaacta cgtggtagga
     2221 gaggaccaac caactctgga aatctgttct gcaaacacat gctcacacac acacacacaa
     2281 atagtataaa caattttaaa tttcatttaa aaataatttg taaacaaaat cattagcaca
     2341 ggttttagaa agagcctctt ggtgacatca agttgatgct gtagatgggg tatcattcct
     2401 gaggacccaa aaccgggtct cagcctttcc ccattctgag agttctctct tttctcagcc
     2461 actagctgaa gagtagagtg gctcagcact gggctcttga gttcccaagt cctacaactg
     2521 gtcagcctga ctactaacca gccatgaaga aacaaggagt ggatgggctg agtctgctgg
     2581 gatgggagtg gagttagtaa gtggccatgg atgtaatgac cccagcaatg ctggctagaa
     2641 ggcatgcctc ctttccttgt ctggagacgg aacgggatca tcttgtactc acagaaggga
     2701 gaacattcta gctggttggg ccaaaatgtg caagttcacc tggaggtggt ggtgcatgct
     2761 tttaactcca gtactcagga ggcagggcca ggtggatctc tgtgagttca agaccagcct
     2821 gcactatgga gagagttttg ggacagccag agttacacag aaaaatcctg gtggaaaatc
     2881 tgaaagaaag agagaaagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa
     2941 agaaagaaag aaagaaagga agaaagaaag aaagagtggc aggcaggcag gcaggaggaa
     3001 ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa
     3061 aataggtgcg acttcaagat ccggagttac aagcagaatg cactgtttcc ctaacagggc
     3121 caagtgtttt gagtaactga aggtgggcat gatgcctggg aagcagaaac aagccaggca
     3181 gatgcacccc ttgccttgct tccgaagggc tgcagtagca tggaaaacat ggaaaacaac
     3241 caatccattc cctttgctga tataacaggc tccaaagcca aaacctgtca ctggaggctc
     3301 aagagcagat ctccagccaa gaggcaaagg aatgggggaa gctggagggc ctccctctgg
     3361 ttatccaggc ttctgaaggt tcaagcaaag aaagggttac aaccttaaaa ggagagcgtc
     3421 ccggggtatg ggtagaagac tgctccaccc cgacccccag ggtccctaac cgtcttttcc
     3481 ctgggcgagt cagcccaatc acaggactga gagtgcctct ttagtagcag caagccactt
     3541 cggacaccca aatggaacac ctccagtcag ccctcgccga ccaccccacc ccctccatcc
     3601 ttttccctca gcctccgatt ggctgaatct agagtccctc cctgctcccc cctctctccc
     3661 cacccctggt aaaactgcgg gcttcaggct gggtgcagca actggaggcg ttggcgacca
     3721 ggaggaggct gcagctaggg gagtccaggt gagagcaggc cgacgggagg gacccgcaca
     3781 tgcaaggacc gccgcagggc gaggatgcaa gccttcccca gctacagttt tgggaaagga
     3841 taccagggcg ctcctatatg ggggcgcggg aacctgggga aagaaggtgc tcccaggtcg
     3901 aggtgggaga ggaaggcagt gcggggtcac gggctttctc cctgctaacg gacgctttcg
     3961 aagagtgggt gccggagaga accatgagga aggacatcaa ggacagcctt tggtccccaa
     4021 gctcaaatcg ctttagtggt gcgaatagag ggaggaggtg ggtggcaaac tggagggagt
     4081 ccccagcggt gacctcgtgg ctggctggtg cggggcacca ggtaagaaac gcaatgttgc
     4141 gggaggggac tgggtggcag gcgcggggga ggggaaagct agaaaggatg cgagggagcg
     4201 gaggggggag ggagcggggg aatctcaact ggtagaggaa agttaaaatg aggaaatagc
     4261 atcagggtgg ggttagccaa gccgggcctc agggaaaggg cgcaaagttt gtctgggtgt
     4321 gggcttaggt gggctgggta tgagattcgg ggcgccgaaa acactgctgc gcctctgcca
     4381 aatcacgcta cccctgtatc tagttctgct aggcttctcc agccccagcc ccaattcttt
     4441 tctcagtgtc cccttccctc ccctgaatct caagcccaca ctccctcctc cataacccac
     4501 tgttatcaaa tctaagtcat ttgccaccca acaaccatca ggaggcggaa gcagacggga
     4561 ggagtttgag atcaacttgg gctacatcac gagttccaag ctcaccaagg cttcttaagg
     4621 agaccttgtc tctaaaatta attaattaat taattaatag tcccctttct ctgccacaga
     4681 accttgggat ctggctcctg gtcgcagctc cccccacccc aggctgacat tcactgccat
     4741 agcccatccg gaaatcctag tctattcccc atggatcttg aactgcagag agaatggcag
     4801 agtggcccgc cctgtgcaaa ggatgttcct agcctaggtg gagctcgcga actcggagac
     4861 tgtgcctctc ttgggcaagg acaggctaga cagcctgccg gtgttgagct agggcactgt
     4921 ggggaaggca gagaacctgt gcagggcacg caatgaacac aggaccagaa aactgcagcc
     4981 ctaggaacac tcaagagctg gccatttgca agcatctctg gcctccgtgc ttctcactca
     5041 tgtcccatgt cttatacagg cctctgtggc acctcgcttg cctgatctca tccctagccg
     5101 ttaagctttc tgcatgactt atcacttggg gcataatgct ggatacctac cattttctta
     5161 gaccccatca aaatcctatt tgagtgtacg gttcggagaa ctccttattt atccggtaaa
     5221 tgtcttttac tctgctctca gggagctgag gcaggacatt cctgagatac attgggagag
     5281 gaatacagtt tcaataaaat aataggttgg gtggaggtac atgcctataa tgccaccact
     5341 caggaaatgg tggcagcttc gtgagtttga ggccaaccca agaaacatag tgaaaccctg
     5401 tcagtaaata agtaagcaag tatttgagta tctactatat gctagggctg acctggacat
     5461 taggggtcat cttctgaaca aactagtgct tgagggaggt atttggggtt tttgtttgtt
     5521 taatggatct gaatgagttc cagagactgg ctacacagcg atatgactga gcttaacacc
     5581 cctaaagcat acagtcagac caattagaca ataaaaggta tgtatagctt accaaataaa
     5641 aaaattgtat tttcaagaga gtgtctgtct gtgtagccct ggctgttctt gaactcactc
     5701 tgtagaccag gctggcctgg aaatccatct gcctctgcct ctctgcctct ctgcctctct
     5761 gcctctctct ctgcctctct ctgcctctct ctgcccctct ctgcccctct ctgcccctct
     5821 ctgccgccct ctgccttttg ccctctgccc tctgttctct ggcctctgcc ctctccctgc
     5881 cttctccctc tgcctctgcc tcttgagtgc tggaatcaaa ggtgtgagct ctataggtct
     5941 taagttccag aagaaacgta atgaagtcac ccagcaggag gtgctcaggg acagcaagac
     6001 acacacaccc aggacactag gctcccactt ccttggcttt ctctgagtgg caaaggacct
     6061 taggcagtgt cactccctaa gagaagggga taaagagagg ggctgaggta ttcagtcatg
     6121 tgctccgtgg atctcaagcc ctcaaggtaa atggggaccc acctgtccta ccagctggct
     6181 gacctgtagc tttccccacc acagaatcca agtcggaact cttggcacct agaggatctc
     6241 gactagcgag ctcataactt cgtataggat actttatacg aagttataac tcgagataac
     6301 ttcgtatagc atacattata cgaagttata ccggcagatc tgccaccatg gtgagcaagg
     6361 gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg
     6421 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     6481 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc
     6541 agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga
     6601 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc
     6661 tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga
     6721 tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg
     6781 aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg
     6841 ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca
     6901 agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac atcacctccc
     6961 acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt
     7021 tcctgtacgg catggacgag ctgtacaagt aaagtgtgcg gccgcgactc tagatcataa
     7081 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     7141 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     7201 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7261 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt
     7321 aatgctccga gggccgccac cacctgttcc tgtaggaatt ggccgcgact ctaggatcta
     7381 ggataacttc gtataggata ctttatacga agttattgat ccaccggtcg ccaccatggt
     7441 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     7501 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     7561 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     7621 gaccaccctg acctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     7681 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     7741 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     7801 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     7861 ggagtacaac gccatcagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     7921 caaggccaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     7981 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     8041 gagcacccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     8101 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg
     8161 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     8221 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     8281 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8341 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     8401 tcgagataac ttcgtatagc atacattata cgaagttata ccggtcgcca ccatggtgag
     8461 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     8521 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     8581 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     8641 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
     8701 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     8761 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     8821 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     8881 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     8941 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     9001 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     9061 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     9121 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg
     9181 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     9241 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     9301 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     9361 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
     9421 gtaaattgta agcgttaatt cgaggtcctt cctctgcaga ggtcttgctt ctcccggtca
     9481 gctgactccc tccccaagtc cttcaaatat ctcagaacat ggggagaaac ggggaccttg
     9541 tccctcctaa ggaaccccag tgctgcatgc catcatcccc cccaccctcg cccccacccc
     9601 cgccacttct ccctccatgc ataccactag ctgtcatttt gtactctgta tttattctag
     9661 ggctgcttct gattatttag tttgttcttt ccctggagac ctgttagaac ataagggcgt
     9721 atggtgggta ggggaggcag gatatcagtc ccctggggcg agttcctccc tgccaaccaa
     9781 gccagatgcc tgaaagagat atggatgagg gaagttggac tgtgcctgta cctggtacag
     9841 tcatactctg ttgaaagaat catcggggag gggggggggc tcaagagggg agagctctct
     9901 gagcctttgt ggaccatcca atgaggatga gggcttagat tctaccaggt cattctcagc
     9961 caccacacac aagcgctctg ccatcactga agaagccccc tagggctctt gggccagggc
    10021 acactcagta aagatgcagg ttcagtcagg gaatgatggg gaaaggggta ggaggtgggg
    10081 gagggatcac cccctcctct aaaacacgag cctgctgtct ccaaaggcct ctgcctgtag
    10141 tgagggtggc agaagaagac aaggagccag aactctgact ccaggatcta agtccgtgca
    10201 ggaaggggat cctagaacca tctggttgga cccagcttac caagggagag cctttattcc
    10261 ttctttccct tgcccctctg tgccagcccc tcttgctgtc cctgatcccc cagacagcga
    10321 gagtcttgca acctgcctct tccaagacct cctaatctca ggggcaggcg gtggagtgag
    10381 atccggcgtg cacacttttt ggaagatagc tttcccaagg atcctctccc ccactggcag
    10441 ctctgcctgt cccatcacca tgtataatac caccactgct acagcatctc accgaggaaa
    10501 gaaaactgca caataaaacc aagcctctgg agtgtgtcct ggtgtctgtc tcttctgtgt
    10561 cctggcgtct gtctcttctg tgttcttcca aggtcagaaa caaaaaccac acacttcaac
    10621 ctggatggct cggctgagca cttctgtgtg cagaaggtcc aaccagactc tggggtaccc
    10681 cggccctccc tattcccttg cctcctgtct cccgcttttt atagctccct atgctgggct
    10741 tctctggaga gtgaaatctt tgcccaaatc aatgcgcatt ctctctgctg agtcatctgg
    10801 cgacagcagt tgagttcacc cgccaacaca tgggcccagc tatgtagccg aaccctggct
    10861 ctggaagtgc cagggacttt gtgcataagt atgtaccatg cccttttttc acagtcctag
    10921 ctctgcagaa gtgcagcctg aaggcctgtc tgctgagagg acatgccctg gagccctgaa
    10981 acaggcacag tgggaggagg aacggaggat gacaggcatc aggccctcag tccaaaagca
    11041 accacttgag aatgggctgg agtacgaaac atggggtccc gtccctggat ccctcctcaa
    11101 agagtaataa gtaaaatata aacaggtacc ccaggccgtt ctgggtttgg gttgtaatgg
    11161 gatccatttg cagagaacta ttgagacagc ccagccgtac tgtgacaggc aatgtggggg
    11221 aggaggttga atcacttggt atttagcatg aatagaataa ttccctgaac atttttctta
    11281 aacatccata tctaaattac caccactcgc tcccagtctt cctgcctttg cgccagcctc
    11341 ctgtctggcc atgcctgaag aaggctggag aagccaccca cctcaggcca tgacactgcc
    11401 agccacttgg caggtgcagc caaacctgag ctgtcccaga aagggacatt ctcaagaccc
    11461 aggcaccctg atcagcactg acttggagct acaagtgtca tgccagaaaa gtctctaaga
    11521 aaaccttttc agggaaaagg gggtgactca acaccgggca agtttgggaa gccccaccct
    11581 tcgagtgatg gaagagcaga taggaagcct cagaagagag acaccggcac ccaggtaacg
    11641 ttcctcatgt ggtctctgtc acactaggtg ctcttccctg gacatctccg tgaccacact
    11701 ctcagttctt agggagatgc gggtgctctc tgaggctatc tcagagttgc agattctgag
    11761 gcctagagtg actacagtca gcctaggaag ccacagagga ctgtggacca ggagggcaga
    11821 agaggagaag ggaagaaaaa ccatcagata ggacttgcaa tgaaactaac ccaagacaat
    11881 cataatgcag acaggaatgt taaaggcgtt cagcagctgg ccatgacacc catctgtccc
    11941 tctggccaag tcagcaagcc tggaagacct gggactcctg cccatatgtc ctaagctccc
    12001 cacccaccca ctcgttcact gtccttattc tctctctacc ttcagccact tagtttccta
    12061 ccttaagtcc tagaattgat cctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
    12121 ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca
    12181 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc
    12241 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
    12301 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
    12361 gttttcaccg tcatcaccga aacgcgcga
//
