LOCUS       sequence-88440-e        2156 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2156)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..2156
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..708
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gtgtgattaa accagacatg
       61 aagatcaagc tgcgtatgga aggcgctgta aatggacacc cgttcgcgat tgaaggagtt
      121 ggccttggga agcctttcga gggaaaacag agtatggacc ttaaagtcaa agaaggcgga
      181 cctctgcctt tcgcctatga catcttgaca actgtgttct gttacggcaa cagggtattc
      241 gccaaatacc cagaaaatat agtagactat ttcaagcagt cgtttcctga gggctactct
      301 tgggaacgaa gcatgaatta cgaagacggg ggcatttgta acgcgacaaa cgacataacc
      361 ctggatggtg actgttatat ctatgaaatt cgatttgatg gtgtgaactt tcctgccaat
      421 ggtccagtta tgcagaagag gactgtgaaa tgggagccat ccactgagaa attgtatgtg
      481 cgtgatggag tgctgaaggg tgatgttaac atggctctgt cgcttgaagg aggtggccat
      541 taccgatgtg acttcaaaac tacttataaa gctaagaagg ttgtccagtt gccagactat
      601 cactttgtgg accaccacat tgagattaaa agccacgaca aagattacag taatgttaat
      661 ctgcatgagc acgccgaagc gcattctgag ctgccgaggc aggccaagtc cggactcaga
      721 tctggcagcg gtggaggcag cgcatccggc ggaagcggaa gcgtgcgtga gtgcatctcc
      781 atccacgttg gccaggctgg tgtccagatt ggcaatgcct gctgggagct ctactgcctg
      841 gaacacggca tccagcccga tggccagatg ccaagtgaca agaccattgg gggaggagat
      901 gactccttca acaccttctt cagtgagacg ggcgctggca agcacgtgcc ccgggctgtg
      961 tttgtagact tggaacccac agtcattgat gaagttcgca ctggcaccta ccgccagctc
     1021 ttccaccctg agcagctcat cacaggcaag gaagatgctg ccaataacta tgcccgaggg
     1081 cactacacca ttggcaagga gatcattgac cttgtgttgg accgaattcg caagctggct
     1141 gaccagtgca ccggtcttca gggcttcttg gttttccaca gctttggtgg gggaactggt
     1201 tctgggttca cctccctgct catggaacgt ctctcagttg attatggcaa gaagtccaag
     1261 ctggagttct ccatttaccc agcaccccag gtttccacag ctgtagttga gccctacaac
     1321 tccatcctca ccacccacac caccctggag cactctgatt gtgccttcat ggtagacaat
     1381 gaggccatct atgacatctg tcgtagaaac ctcgatatcg agcgcccaac ctacactaac
     1441 cttaaccgcc ttattagcca gattgtgtcc tccatcactg cttccctgag atttgatgga
     1501 gccctgaatg ttgacctgac agaattccag accaacctgg tgccctaccc ccgcatccac
     1561 ttccctctgg ccacatatgc ccctgtcatc tctgctgaga aagcctacca tgaacagctt
     1621 tctgtagcag agatcaccaa tgcttgcttt gagccagcca accagatggt gaaatgtgac
     1681 cctcgccatg gtaaatacat ggcttgctgc ctgttgtacc gtggtgacgt ggttcccaaa
     1741 gatgtcaatg ctgccattgc caccatcaaa accaagcgca gcatccagtt tgtggattgg
     1801 tgccccactg gcttcaaggt tggcatcaac taccagcctc ccactgtggt gcctggtgga
     1861 gacctggcca aggtacagag agctgtgtgc atgctgagca acaccacagc cattgctgag
     1921 gcctgggctc gcctggacca caagtttgac ctgatgtatg ccaagcgtgc ctttgttcac
     1981 tggtacgtgg gtgaggggat ggaggaaggc gagttttcag aggcccgtga agatatggct
     2041 gcccttgaga aggattatga ggaggttggt gtggattctg ttgaaggaga gggtgaggaa
     2101 gaaggagagg aatactaagg atccaccgga tctagataac tgatcataat cagcca
//
