LOCUS       sequence-88444-e        2198 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 503, Emission: 
            518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2198)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Vimentin-C-18
FEATURES             Location/Qualifiers
     source          1..2198
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..702
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagtgtga ttaaaccaga catgaagatc
       61 aagctgcgta tggaaggcgc tgtaaatgga cacccgttcg cgattgaagg agttggcctt
      121 gggaagcctt tcgagggaaa acagagtatg gaccttaaag tcaaagaagg cggacctctg
      181 cctttcgcct atgacatctt gacaactgtg ttctgttacg gcaacagggt attcgccaaa
      241 tacccagaaa atatagtaga ctatttcaag cagtcgtttc ctgagggcta ctcttgggaa
      301 cgaagcatga attacgaaga cgggggcatt tgtaacgcga caaacgacat aaccctggat
      361 ggtgactgtt atatctatga aattcgattt gatggtgtga actttcctgc caatggtcca
      421 gttatgcaga agaggactgt gaaatgggag ccatccactg agaaattgta tgtgcgtgat
      481 ggagtgctga agggtgatgt taacatggct ctgtcgcttg aaggaggtgg ccattaccga
      541 tgtgacttca aaactactta taaagctaag aaggttgtcc agttgccaga ctatcacttt
      601 gtggaccacc acattgagat taaaagccac gacaaagatt acagtaatgt taatctgcat
      661 gagcacgccg aagcgcattc tgagctgccg aggcaggcca agtccggact cagatctggc
      721 agcggtggag gcagcgcatc cggcggaagc ggaagctcca ccaggtccgt gtcctcgtcc
      781 tcctaccgca ggatgttcgg cggcccgggc accgcgagcc ggccgagctc cagccggagc
      841 tacgtgacta cgtccacccg cacctacagc ctgggcagcg cgctgcgccc cagcaccagc
      901 cgcagcctct acgcctcgtc cccgggcggc gtgtatgcca cgcgctcctc tgccgtgcgc
      961 ctgcggagca gcgtgcccgg ggtgcggctc ctgcaggact cggtggactt ctcgctggcc
     1021 gacgccatca acaccgagtt caagaacacc cgcaccaacg agaaggtgga gctgcaggag
     1081 ctgaatgacc gcttcgccaa ctacatcgac aaggtgcgct tcctggagca gcagaataag
     1141 atcctgctgg ccgagctcga gcagctcaag ggccaaggca agtcgcgcct gggggacctc
     1201 tacgaggagg agatgcggga gctgcgccgg caggtggacc agctaaccaa cgacaaagcc
     1261 cgcgtcgagg tggagcgcga caacctggcc gaggacatca tgcgcctccg ggagaaattg
     1321 caggaggaga tgcttcagag agaggaagcc gaaaacaccc tgcaatcttt cagacaggat
     1381 gttgacaatg cgtctctggc acgtcttgac cttgaacgca aagtggaatc tttgcaagaa
     1441 gagattgcct ttttgaagaa actccacgaa gaggaaatcc aggagctgca ggctcagatt
     1501 caggaacagc atgtccaaat cgatgtggat gtttccaagc ctgacctcac ggctgccctg
     1561 cgtgacgtac gtcagcaata tgaaagtgtg gctgccaaga acctgcagga ggcagaagaa
     1621 tggtacaaat ccaagtttgc tgacctctct gaggctgcca accggaacaa tgacgccctg
     1681 cgccaggcaa agcaggagtc cactgagtac cggagacagg tgcagtccct cacctgtgaa
     1741 gtggatgccc ttaaaggaac caatgagtcc ctggaacgcc agatgcgtga aatggaagag
     1801 aactttgccg ttgaagctgc taactaccaa gacactattg gccgcctgca ggatgagatt
     1861 cagaatatga aggaggaaat ggctcgtcac cttcgtgaat accaagacct gctcaatgtt
     1921 aagatggccc ttgacattga gattgccacc tacaggaagc tgctggaagg cgaggagagc
     1981 aggatttctc tgcctcttcc aaacttttcc tccctgaacc tgagggaaac taatctggat
     2041 tcactccctc tggttgatac ccactcaaaa aggacacttc tgattaagac ggttgaaact
     2101 agagatggac aggttatcaa cgaaacttct cagcatcacg atgaccttga ataaggatcc
     2161 accggatcta gataactgat cataatcagc cataccac
//
