LOCUS       sequence-8845-ex       13701 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13701)
  AUTHORS   Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, 
            Lichtman JW
  TITLE     Transgenic strategies for combinatorial expression of fluorescent 
            proteins in the nervous system.
  JOURNAL   Nature. 2007 Nov 1. 450(7166):56-62.
  PUBMED    17972876
REFERENCE   2  (bases 1 to 13701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Nov 1. 450(7166):56-62."
COMMENT     Sequence Label: Thy1-Brainbow-1.1 M
FEATURES             Location/Qualifiers
     source          1..13701
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(6253..6286)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(6294..6327)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(6337..6370)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6384..6393
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6390..7046
                     /codon_start=1
                     /product="Kusabira-Orange fluorescent protein (Karasawa et 
                     al., 2004)"
                     /label=Kusabira-Orange
                     /translation="MVSVIKPEMKMKYFMDGSVNGHEFTVEGEGTGKPYEGHQEMTLRV
                     TMAKGGPMPFSFDLVSHTFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLQFEDGGFA
                     AVSAHISLRGNCFEHKSKFVGVNFPADGPVMQNQSSDWEPSTEKITTCDGVLKGDVTMY
                     LKLAGGGNHKCQFKTTYKAAKKILKMPQSHFIGHRLVRKTEGNITELVEDAVAHC"
     polyA_signal    7169..7250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7288..7321)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      7442..7451
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7448..8158
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7593..7611)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7861..7880
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    8286..8407
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(8490..8523)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      8642..8651
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8648..8650,8651..8653,8654..9367)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    9490..9611
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(9626..9659)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      9782..9791
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9788..9790,9791..9793,9794..10507)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    10550..10583
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    10607..10728
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13532..13551)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13651..13673
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcaggatc gcggccgcga tccccgggcg agctcgaatt cagagaccgg
     2101 gaaccaaact agcctttaaa aaacataagt acaggagcca gcaagatggc tcagtgggta
     2161 aaggtgccta ccagcaagcc tgacagcctg agttcagtcc ccacgaacta cgtggtagga
     2221 gaggaccaac caactctgga aatctgttct gcaaacacat gctcacacac acacacacaa
     2281 atagtataaa caattttaaa tttcatttaa aaataatttg taaacaaaat cattagcaca
     2341 ggttttagaa agagcctctt ggtgacatca agttgatgct gtagatgggg tatcattcct
     2401 gaggacccaa aaccgggtct cagcctttcc ccattctgag agttctctct tttctcagcc
     2461 actagctgaa gagtagagtg gctcagcact gggctcttga gttcccaagt cctacaactg
     2521 gtcagcctga ctactaacca gccatgaaga aacaaggagt ggatgggctg agtctgctgg
     2581 gatgggagtg gagttagtaa gtggccatgg atgtaatgac cccagcaatg ctggctagaa
     2641 ggcatgcctc ctttccttgt ctggagacgg aacgggatca tcttgtactc acagaaggga
     2701 gaacattcta gctggttggg ccaaaatgtg caagttcacc tggaggtggt ggtgcatgct
     2761 tttaactcca gtactcagga ggcagggcca ggtggatctc tgtgagttca agaccagcct
     2821 gcactatgga gagagttttg ggacagccag agttacacag aaaaatcctg gtggaaaatc
     2881 tgaaagaaag agagaaagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa
     2941 agaaagaaag aaagaaagga agaaagaaag aaagagtggc aggcaggcag gcaggaggaa
     3001 ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa
     3061 aataggtgcg acttcaagat ccggagttac aagcagaatg cactgtttcc ctaacagggc
     3121 caagtgtttt gagtaactga aggtgggcat gatgcctggg aagcagaaac aagccaggca
     3181 gatgcacccc ttgccttgct tccgaagggc tgcagtagca tggaaaacat ggaaaacaac
     3241 caatccattc cctttgctga tataacaggc tccaaagcca aaacctgtca ctggaggctc
     3301 aagagcagat ctccagccaa gaggcaaagg aatgggggaa gctggagggc ctccctctgg
     3361 ttatccaggc ttctgaaggt tcaagcaaag aaagggttac aaccttaaaa ggagagcgtc
     3421 ccggggtatg ggtagaagac tgctccaccc cgacccccag ggtccctaac cgtcttttcc
     3481 ctgggcgagt cagcccaatc acaggactga gagtgcctct ttagtagcag caagccactt
     3541 cggacaccca aatggaacac ctccagtcag ccctcgccga ccaccccacc ccctccatcc
     3601 ttttccctca gcctccgatt ggctgaatct agagtccctc cctgctcccc cctctctccc
     3661 cacccctggt aaaactgcgg gcttcaggct gggtgcagca actggaggcg ttggcgacca
     3721 ggaggaggct gcagctaggg gagtccaggt gagagcaggc cgacgggagg gacccgcaca
     3781 tgcaaggacc gccgcagggc gaggatgcaa gccttcccca gctacagttt tgggaaagga
     3841 taccagggcg ctcctatatg ggggcgcggg aacctgggga aagaaggtgc tcccaggtcg
     3901 aggtgggaga ggaaggcagt gcggggtcac gggctttctc cctgctaacg gacgctttcg
     3961 aagagtgggt gccggagaga accatgagga aggacatcaa ggacagcctt tggtccccaa
     4021 gctcaaatcg ctttagtggt gcgaatagag ggaggaggtg ggtggcaaac tggagggagt
     4081 ccccagcggt gacctcgtgg ctggctggtg cggggcacca ggtaagaaac gcaatgttgc
     4141 gggaggggac tgggtggcag gcgcggggga ggggaaagct agaaaggatg cgagggagcg
     4201 gaggggggag ggagcggggg aatctcaact ggtagaggaa agttaaaatg aggaaatagc
     4261 atcagggtgg ggttagccaa gccgggcctc agggaaaggg cgcaaagttt gtctgggtgt
     4321 gggcttaggt gggctgggta tgagattcgg ggcgccgaaa acactgctgc gcctctgcca
     4381 aatcacgcta cccctgtatc tagttctgct aggcttctcc agccccagcc ccaattcttt
     4441 tctcagtgtc cccttccctc ccctgaatct caagcccaca ctccctcctc cataacccac
     4501 tgttatcaaa tctaagtcat ttgccaccca acaaccatca ggaggcggaa gcagacggga
     4561 ggagtttgag atcaacttgg gctacatcac gagttccaag ctcaccaagg cttcttaagg
     4621 agaccttgtc tctaaaatta attaattaat taattaatag tcccctttct ctgccacaga
     4681 accttgggat ctggctcctg gtcgcagctc cccccacccc aggctgacat tcactgccat
     4741 agcccatccg gaaatcctag tctattcccc atggatcttg aactgcagag agaatggcag
     4801 agtggcccgc cctgtgcaaa ggatgttcct agcctaggtg gagctcgcga actcggagac
     4861 tgtgcctctc ttgggcaagg acaggctaga cagcctgccg gtgttgagct agggcactgt
     4921 ggggaaggca gagaacctgt gcagggcacg caatgaacac aggaccagaa aactgcagcc
     4981 ctaggaacac tcaagagctg gccatttgca agcatctctg gcctccgtgc ttctcactca
     5041 tgtcccatgt cttatacagg cctctgtggc acctcgcttg cctgatctca tccctagccg
     5101 ttaagctttc tgcatgactt atcacttggg gcataatgct ggatacctac cattttctta
     5161 gaccccatca aaatcctatt tgagtgtacg gttcggagaa ctccttattt atccggtaaa
     5221 tgtcttttac tctgctctca gggagctgag gcaggacatt cctgagatac attgggagag
     5281 gaatacagtt tcaataaaat aataggttgg gtggaggtac atgcctataa tgccaccact
     5341 caggaaatgg tggcagcttc gtgagtttga ggccaaccca agaaacatag tgaaaccctg
     5401 tcagtaaata agtaagcaag tatttgagta tctactatat gctagggctg acctggacat
     5461 taggggtcat cttctgaaca aactagtgct tgagggaggt atttggggtt tttgtttgtt
     5521 taatggatct gaatgagttc cagagactgg ctacacagcg atatgactga gcttaacacc
     5581 cctaaagcat acagtcagac caattagaca ataaaaggta tgtatagctt accaaataaa
     5641 aaaattgtat tttcaagaga gtgtctgtct gtgtagccct ggctgttctt gaactcactc
     5701 tgtagaccag gctggcctgg aaatccatct gcctctgcct ctctgcctct ctgcctctct
     5761 gcctctctct ctgcctctct ctgcctctct ctgcccctct ctgcccctct ctgcccctct
     5821 ctgccgccct ctgccttttg ccctctgccc tctgttctct ggcctctgcc ctctccctgc
     5881 cttctccctc tgcctctgcc tcttgagtgc tggaatcaaa ggtgtgagct ctataggtct
     5941 taagttccag aagaaacgta atgaagtcac ccagcaggag gtgctcaggg acagcaagac
     6001 acacacaccc aggacactag gctcccactt ccttggcttt ctctgagtgg caaaggacct
     6061 taggcagtgt cactccctaa gagaagggga taaagagagg ggctgaggta ttcagtcatg
     6121 tgctccgtgg atctcaagcc ctcaaggtaa atggggaccc acctgtccta ccagctggct
     6181 gacctgtagc tttccccacc acagaatcca agtcggaact cttggcacct agaggatctc
     6241 gactagctcg agataacttc gtatagtata ccttatacga agttatcgag ctcataactt
     6301 cgtataggat actttatacg aagttataac gtcgagataa cttcgtatag catacattat
     6361 acgaagttat accggcagat ctcgccacca tggtgagtgt gattaaacca gagatgaaga
     6421 tgaagtactt catggacggc tccgtcaatg ggcatgagtt cacagttgaa ggtgaaggca
     6481 caggcaaacc ttacgaggga catcaagaga tgacactacg cgtcacaatg gccaagggcg
     6541 ggccaatgcc tttctcgttt gacttagtgt cacacacgtt ctgttacggc cacagacctt
     6601 ttactaaata tccagaagag ataccagact atttcaaaca agcatttcct gaaggcctgt
     6661 catgggaaag gtcgttgcag ttcgaagatg gtgggttcgc tgccgtcagt gcgcatataa
     6721 gccttagagg aaactgcttc gagcacaaat ccaaatttgt tggggttaac tttcctgccg
     6781 atggtcctgt gatgcaaaac caaagttctg attgggagcc atcaaccgag aaaattacta
     6841 cctgcgacgg agttctgaag ggtgatgtta cgatgtacct aaaacttgcg ggaggcggca
     6901 atcacaaatg ccaattcaag actacttaca aggcggcaaa aaagattctt aaaatgccac
     6961 aaagccattt catcgggcat cgcctcgtca ggaaaaccga aggcaacatt actgagctgg
     7021 tagaagatgc agtagctcat tgctgaagcg gccgcgactc tagatcataa tcagccatac
     7081 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     7141 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     7201 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7261 tggtttgtcc aaactcatca atgtatcata acttcgtata gtatacctta tacgaagtta
     7321 taggatctcc gccaccatgc tgtgctgcat cagaagaact aaaccggttg agaagaatga
     7381 agaggccgat caggagctgc agtcgacggt accgcgggcc cgggatccac cggtagcatc
     7441 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     7501 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     7561 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     7621 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     7681 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     7741 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     7801 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     7861 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     7921 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     7981 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     8041 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     8101 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaaac
     8161 acgtgcggcc gcgactctag atcataatca gccataccac atttgtagag gttttacttg
     8221 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     8281 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8341 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8401 tatcttaagg cgtaaattgt aagcgttaat gctccgaggg ccgccaccac ctgttcctgt
     8461 aggaattggc cgcgactcta ggatctagga taacttcgta taggatactt tatacgaagt
     8521 tattgatcaa ttcacagcca ccatgctgtg ctgcatcaga agaactaaac cggttgagaa
     8581 gaatgaagag gccgatcagg agctgcagtc gacggtaccg cgggcccggg atccaccggt
     8641 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     8701 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     8761 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     8821 gcccaccctc gtgaccacct tcggctacgg cctgcagtgc ttcgcccgct accccgacca
     8881 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     8941 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     9001 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     9061 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     9121 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     9181 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     9241 caaccactac ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     9301 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     9361 caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
     9421 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     9481 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9541 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9601 aatgtatctt aacgcgcgat tcgagataac ttcgtatagc atacattata cgaagttata
     9661 ccggaattaa ttcacagcca ccatgctgtg ctgcatcaga agaactaaac cggttgagaa
     9721 gaatgaagag gccgatcagg agctgcagtc gacggtaccg cgggcccggg atccaccggt
     9781 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     9841 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     9901 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     9961 gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct accccgacca
    10021 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
    10081 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
    10141 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
    10201 ggggcacaag ctggagtaca acgccatcag cgacaacgtc tatatcaccg ccgacaagca
    10261 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca
    10321 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
    10381 caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca
    10441 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
    10501 caagtaaagc ggccgcgact ctagatcacc gcgactctag atcacgcgcg aagttcctat
    10561 tctctagaaa gtataggaac ttctcgagta ctcaattgtt gttgttaact tgtttattgc
    10621 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10681 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
    10741 taagcgttaa ttcgaggtcc ttcctctgca gaggtcttgc ttctcccggt cagctgactc
    10801 cctccccaag tccttcaaat atctcagaac atggggagaa acggggacct tgtccctcct
    10861 aaggaacccc agtgctgcat gccatcatcc cccccaccct cgcccccacc cccgccactt
    10921 ctccctccat gcataccact agctgtcatt ttgtactctg tatttattct agggctgctt
    10981 ctgattattt agtttgttct ttccctggag acctgttaga acataagggc gtatggtggg
    11041 taggggaggc aggatatcag tcccctgggg cgagttcctc cctgccaacc aagccagatg
    11101 cctgaaagag atatggatga gggaagttgg actgtgcctg tacctggtac agtcatactc
    11161 tgttgaaaga atcatcgggg aggggggggg gctcaagagg ggagagctct ctgagccttt
    11221 gtggaccatc caatgaggat gagggcttag attctaccag gtcattctca gccaccacac
    11281 acaagcgctc tgccatcact gaagaagccc cctagggctc ttgggccagg gcacactcag
    11341 taaagatgca ggttcagtca gggaatgatg gggaaagggg taggaggtgg gggagggatc
    11401 accccctcct ctaaaacacg agcctgctgt ctccaaaggc ctctgcctgt agtgagggtg
    11461 gcagaagaag acaaggagcc agaactctga ctccaggatc taagtccgtg caggaagggg
    11521 atcctagaac catctggttg gacccagctt accaagggag agcctttatt ccttctttcc
    11581 cttgcccctc tgtgccagcc cctcttgctg tccctgatcc cccagacagc gagagtcttg
    11641 caacctgcct cttccaagac ctcctaatct caggggcagg cggtggagtg agatccggcg
    11701 tgcacacttt ttggaagata gctttcccaa ggatcctctc ccccactggc agctctgcct
    11761 gtcccatcac catgtataat accaccactg ctacagcatc tcaccgagga aagaaaactg
    11821 cacaataaaa ccaagcctct ggagtgtgtc ctggtgtctg tctcttctgt gtcctggcgt
    11881 ctgtctcttc tgtgttcttc caaggtcaga aacaaaaacc acacacttca acctggatgg
    11941 ctcggctgag cacttctgtg tgcagaaggt ccaaccagac tctggggtac cccggccctc
    12001 cctattccct tgcctcctgt ctcccgcttt ttatagctcc ctatgctggg cttctctgga
    12061 gagtgaaatc tttgcccaaa tcaatgcgca ttctctctgc tgagtcatct ggcgacagca
    12121 gttgagttca cccgccaaca catgggccca gctatgtagc cgaaccctgg ctctggaagt
    12181 gccagggact ttgtgcataa gtatgtacca tgcccttttt tcacagtcct agctctgcag
    12241 aagtgcagcc tgaaggcctg tctgctgaga ggacatgccc tggagccctg aaacaggcac
    12301 agtgggagga ggaacggagg atgacaggca tcaggccctc agtccaaaag caaccacttg
    12361 agaatgggct ggagtacgaa acatggggtc ccgtccctgg atccctcctc aaagagtaat
    12421 aagtaaaata taaacaggta ccccaggccg ttctgggttt gggttgtaat gggatccatt
    12481 tgcagagaac tattgagaca gcccagccgt actgtgacag gcaatgtggg ggaggaggtt
    12541 gaatcacttg gtatttagca tgaatagaat aattccctga acatttttct taaacatcca
    12601 tatctaaatt accaccactc gctcccagtc ttcctgcctt tgcgccagcc tcctgtctgg
    12661 ccatgcctga agaaggctgg agaagccacc cacctcaggc catgacactg ccagccactt
    12721 ggcaggtgca gccaaacctg agctgtccca gaaagggaca ttctcaagac ccaggcaccc
    12781 tgatcagcac tgacttggag ctacaagtgt catgccagaa aagtctctaa gaaaaccttt
    12841 tcagggaaaa gggggtgact caacaccggg caagtttggg aagccccacc cttcgagtga
    12901 tggaagagca gataggaagc ctcagaagag agacaccggc acccaggtaa cgttcctcat
    12961 gtggtctctg tcacactagg tgctcttccc tggacatctc cgtgaccaca ctctcagttc
    13021 ttagggagat gcgggtgctc tctgaggcta tctcagagtt gcagattctg aggcctagag
    13081 tgactacagt cagcctagga agccacagag gactgtggac caggagggca gaagaggaga
    13141 agggaagaaa aaccatcaga taggacttgc aatgaaacta acccaagaca atcataatgc
    13201 agacaggaat gttaaaggcg ttcagcagct ggccatgaca cccatctgtc cctctggcca
    13261 agtcagcaag cctggaagac ctgggactcc tgcccatatg tcctaagctc cccacccacc
    13321 cactcgttca ctgtccttat tctctctcta ccttcagcca cttagtttcc taccttaagt
    13381 cctagaattg atcctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
    13441 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
    13501 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
    13561 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
    13621 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
    13681 cgtcatcacc gaaacgcgcg a
//
