LOCUS       sequence-88453-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 508 / 572, Emission: 518 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Kaede-Nucleus-9
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        24..75
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     26..46
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             113..790
                     /codon_start=1
                     /product="Kaede green-to-red photoconvertible fluorescent 
                     protein (Ando et al., 2002)"
                     /label=Kaede
                     /translation="MVSLIKPEMKIKLLMEGNVNGHQFVIEGDGKGHPFEGKQSMDLVV
                     KEGAPLPFAYDILTTAFHYGNRVFAKYPDHIPDYFKQSFPKGFSWERSLMFEDGGVCIA
                     TNDITLKGDTFFNKVRFDGVNFPPNGPVMQKKTLKWEASTEKMYLRDGVLTGDITMALL
                     LKGDVHYRCDFRTTYKSRQEGVKLPGYHFVDHCISILRHDKDYNEVKLYEHAVAHSGLP
                     DNVK"
     CDS             821..841
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             845..865
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             869..889
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
       61 tatataagca gagctggttt agtgaaccgt cagatccgct agcattgcca ccatggtgag
      121 tctgattaaa ccagaaatga agatcaagct gcttatggaa ggcaatgtaa acgggcacca
      181 gtttgttatt gagggagatg gaaaaggcca tccttttgag ggaaaacaga gtatggacct
      241 tgtagtcaaa gaaggcgcac ctctcccttt tgcctacgat atcttgacaa cagcattcca
      301 ttatggtaac agggtttttg ctaaataccc agaccatata ccagactact tcaagcagtc
      361 gtttcccaaa gggttttctt gggagcgaag cctgatgttc gaggacgggg gcgtttgcat
      421 cgctacaaat gacataacac tgaaaggaga cacttttttt aacaaagttc gatttgatgg
      481 cgtaaacttt cccccaaatg gtcctgttat gcagaagaag actctgaaat gggaggcatc
      541 cactgagaaa atgtatttgc gtgatggagt gttgacgggc gatattacaa tggctctgct
      601 gcttaaagga gatgtccatt accgatgtga cttcagaact acttacaaat ctaggcagga
      661 gggtgtcaag ttgccaggat atcactttgt cgatcactgc atcagcatat tgaggcatga
      721 caaagactac aacgaggtta agctgtatga gcacgctgtt gcccattctg gattgccgga
      781 caacgtcaag ggatcctccg gactcagatc tcgagctgat ccaaaaaaga agagaaaggt
      841 agatccaaaa aagaagagaa aggtagatcc aaaaaagaag agaaaggtaa agcttgaata
      901 agcggccgcg actctagatc ataatcagcc ataccacatt
//
