LOCUS       sequence-88454-e        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Kikume-ER-3
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..59
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     10..30
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             148..828
                     /codon_start=1
                     /product="photoconvertible Kikume green-to-red fluorescent 
                     protein (Tsutsui et al., 2005)"
                     /label=KikGR1
                     /translation="VSVITSEMKIELRMEGAVNGHKFVITGKGSGQPFEGIQNVDLTVI
                     EGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQSFPEGYSWERSMSYEDGGICLAT
                     NNITMKKDGSNCFVNEIRFDGVNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVNMA
                     LLLQGGGHYRCDFRTTYKAKKVVQLPDYHFVDHQMEITSHDKDYNKVKLYEHAKAHSGL
                     PRLAK"
     primer_bind     complement(1037..1056)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
       61 gtttagtgaa ccgtcagatc cgctagcatg ctgctatccg tgccgttgct gctcggcctc
      121 ctcggcctgg ccgtcgccga ccggtcggtg agtgtgatta catcagaaat gaagatcgag
      181 ctgcgtatgg aaggcgctgt aaacgggcac aagttcgtga ttacagggaa aggaagtggc
      241 cagcctttcg agggaataca gaatgtggac ctgacagtca tagagggcgg acctcttcct
      301 tttgctttcg atatcctgac aacagcattc cattacggca accgggtatt tgtcgaatac
      361 ccagaagaaa tagtagacta cttcaagcag tcgtttcctg agggttattc ttgggaacga
      421 agcatgagtt acgaagacgg gggaatttgc ctcgccacaa acaatataac gatgaagaaa
      481 gacggcagca actgttttgt caatgaaatt cgatttgatg gtgtgaactt tcctgccaat
      541 ggtccagtta tgcagaggaa gaccgtcaaa tgggagccat ccactgagaa aatgtatgtg
      601 cgtgatggag tgctgaaggg tgatgtaaac atggctctgt tgcttcaagg aggtggccat
      661 taccgatgtg acttcagaac tacttacaaa gcaaagaagg ttgtccagtt gccagactat
      721 cacttcgtgg atcatcaaat ggagataaca agccatgaca aggattacaa caaggttaag
      781 ctgtatgagc acgctaaagc tcattccggg ctgccaaggc tggccaagga cgagctgtaa
      841 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat aactgatcat
      901 aatcagccat accacatttg tagaggtttt acttgcttta aaaacctccc acacctcccc
      961 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     1021 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttc
//
