LOCUS       sequence-88456-e        1200 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 507 / 583, Emission: 517
            / 593.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1200)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: wtKikGR-H2B-6
FEATURES             Location/Qualifiers
     source          1..1200
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..456
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      469..478
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             475..1161
                     /codon_start=1
                     /product="photoconvertible Kikume green-to-red fluorescent 
                     protein (Tsutsui et al., 2005)"
                     /label=KikGR1
                     /translation="MVSVITSEMKIELRMEGAVNGHKFVITGKGSGQPFEGIQNVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQSFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNCFVNEIRFDGVNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVNM
                     ALLLQGGGHYRCDFRTTYKAKKVVQLPDYHFVDHQMEITSHDKDYNKVKLYEHAKAHSG
                     LPRLAK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgccaccat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc
      121 tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc
      181 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
      241 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
      301 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
      361 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
      421 ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtcgc caccatggtg
      481 agtgtgatta catcagaaat gaagatcgag ctgcgtatgg aaggcgctgt aaacgggcac
      541 aagttcgtga ttacagggaa aggaagtggc cagcctttcg agggaataca gaatgtggac
      601 ctgacagtca tagagggcgg acctcttcct tttgctttcg atatcctgac aacagcattc
      661 cattacggca accgggtatt tgtcgaatac ccagaagaaa tagtagacta cttcaagcag
      721 tcgtttcctg agggttattc ttgggaacga agcatgagtt acgaagacgg gggaatttgc
      781 ctcgccacaa acaatataac gatgaagaaa gacggcagca actgttttgt caatgaaatt
      841 cgatttgatg gtgtgaactt tcctgccaat ggtccagtta tgcagaggaa gaccgtcaaa
      901 tgggagccat ccactgagaa aatgtatgtg cgtgatggag tgctgaaggg tgatgtaaac
      961 atggctctgt tgcttcaagg aggtggccat taccgatgtg acttcagaac tacttacaaa
     1021 gcaaagaagg ttgtccagtt gccagactat cacttcgtgg atcatcaaat ggagataaca
     1081 agccatgaca aggattacaa caaggttaag ctgtatgagc acgctaaagc tcattccggg
     1141 ctgccaaggc tggccaagta agcggccgcg actctagatc ataatcagcc ataccacatt
//
