LOCUS       sequence-88458-e        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 507 / 583, Emission: 517 / 
            593.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKikGR-Mito-7
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..94
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      122..131
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             128..829
                     /codon_start=1
                     /product="monomeric photoconvertible Kikume green-to-red 
                     fluorescent protein"
                     /label=mKikGR1
                     /translation="MVSVITSEMKIELRMEGSVNGHKFVITGKGSGRPYEGTQTVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQLFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNTFVNEIRFDGTNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVEM
                     ALLLEGGGHYRCDFRTTYKAKKVVQLPDYHYVDHQMEITSHDKDYNKVKAYEHAKAYSG
                     TYRGAKYEFEA"
     polyA_signal    951..1029
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ggacgtcaga tccgctagca tgtccgtcct gacgccgctg ctgctgcggg gcttgacagg
       61 ctcggcccgg cggctcccag tgccgcgcgc caagatccat tcgttggggg atccaccggt
      121 cgccaccatg gtgagtgtga ttacatcaga aatgaagatc gagctgcgta tggaaggctc
      181 tgtaaacggg cacaagttcg tgattacagg gaaaggaagt ggcagacctt acgagggaac
      241 acagactgtg gacctgacag tcatagaggg cggacctctt ccttttgctt tcgatatcct
      301 gacaacagca ttccattacg gcaaccgggt atttgtcgaa tacccagaag aaatagtaga
      361 ctacttcaag cagttgtttc ctgagggtta ttcttgggaa cgaagcatga gttacgaaga
      421 cgggggaatt tgcctcgcca caaacaatat aacgatgaag aaagacggca gcaacacttt
      481 tgtcaatgaa attcgatttg atggtacaaa ctttcctgcc aatggtccag ttatgcagag
      541 gaagaccgtc aaatgggagc catccactga gaaaatgtat gtgcgtgatg gagtgctgaa
      601 gggtgatgtt gaaatggctc tgttgcttga aggaggtggc cattaccgat gtgacttcag
      661 aactacttac aaagcaaaga aggttgtcca gttgccagac tatcactacg tggatcatca
      721 aatggagata acaagccatg acaaggatta caacaaggtt aaggcgtatg aacatgctaa
      781 ggcttattcc gggacctaca ggggcgccaa gtatgaattt gaagcctaag cggccgcgac
      841 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
      901 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
      961 ttgcagctta taatggttac aataaagcaa tagcatcaca atttcacaaa taagcatttt
     1021 tttcactgca ttctagttgt gttgtccaac tcatcatg
//
