LOCUS       sequence-88459-e        1698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 507 / 583, Emission: 517 / 593.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1698)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKikGR-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..1698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..49
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             53..73
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             77..97
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             110..808
                     /codon_start=1
                     /product="monomeric photoconvertible Kikume green-to-red 
                     fluorescent protein"
                     /label=mKikGR1
                     /translation="MVSVITSEMKIELRMEGSVNGHKFVITGKGSGRPYEGTQTVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQLFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNTFVNEIRFDGTNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVEM
                     ALLLEGGGHYRCDFRTTYKAKKVVQLPDYHYVDHQMEITSHDKDYNKVKAYEHAKAYSG
                     TYRGAKYEFEA"
ORIGIN
        1 gacgtcagat ccgctagcca ccatggatcc aaaaaagaag agaaaggtag atccaaaaaa
       61 gaagagaaag gtagatccaa aaaagaagag aaaggtaccg gtcgccacca tggtgagtgt
      121 gattacatca gaaatgaaga tcgagctgcg tatggaaggc tctgtaaacg ggcacaagtt
      181 cgtgattaca gggaaaggaa gtggcagacc ttacgaggga acacagactg tggacctgac
      241 agtcatagag ggcggacctc ttccttttgc tttcgatatc ctgacaacag cattccatta
      301 cggcaaccgg gtatttgtcg aatacccaga agaaatagta gactacttca agcagttgtt
      361 tcctgagggt tattcttggg aacgaagcat gagttacgaa gacgggggaa tttgcctcgc
      421 cacaaacaat ataacgatga agaaagacgg cagcaacact tttgtcaatg aaattcgatt
      481 tgatggtaca aactttcctg ccaatggtcc agttatgcag aggaagaccg tcaaatggga
      541 gccatccact gagaaaatgt atgtgcgtga tggagtgctg aagggtgatg ttgaaatggc
      601 tctgttgctt gaaggaggtg gccattaccg atgtgacttc agaactactt acaaagcaaa
      661 gaaggttgtc cagttgccag actatcacta cgtggatcat caaatggaga taacaagcca
      721 tgacaaggat tacaacaagg ttaaggcgta tgaacatgct aaggcttatt ccgggaccta
      781 caggggcgcc aagtatgaat ttgaagcctc cggagaaggg caagggcaag ggcaagggcc
      841 gggccgcggc tacgcgtatc gatccatgtt cgaggcgcgc ctggtccagg gctccatcct
      901 caagaaggtg ttggaggcac tcaaggacct catcaacgag gcctgctggg atattagctc
      961 cagcggtgta aacctgcaga gcatggactc gtcccacgtc tctttggtgc agctcaccct
     1021 gcggtctgag ggcttcgaca cctaccgctg cgaccgcaac ctggccatgg gcgtgaacct
     1081 caccagtatg tccaaaatac taaaatgcgc cggcaatgaa gatatcatta cactaagggc
     1141 cgaagataac gcggatacct tggcgctagt atttgaagca ccaaaccagg agaaagtttc
     1201 agactatgaa atgaagttga tggatttaga tgttgaacaa cttggaattc cagaacagga
     1261 gtacagctgt gtagtaaaga tgccttctgg tgaatttgca cgtatatgcc gagatctcag
     1321 ccatattgga gatgctgttg taatttcctg tgcaaaagac ggagtgaaat tttctgcaag
     1381 tggagaactt ggaaatggaa acattaaatt gtcacagaca agtaatgtcg ataaagagga
     1441 ggaagctgtt accatagaga tgaatgaacc agttcaacta acttttgcac tgaggtacct
     1501 gaacttcttt acaaaagcca ctccactctc ttcaacggtg acactcagta tgtctgcaga
     1561 tgtacccctt gttgtagagt ataaaattgc ggatatggga cacttaaaat actacttggc
     1621 tcccaagatc gaggatgaag aaggatctta gggatccacc ggatctagat aactgatcat
     1681 aatcagccat accacatt
//
