LOCUS       sequence-88460-e        8520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 507 / 583, Emission: 517 
            / 593.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8520)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKikGR-Talin-18
FEATURES             Location/Qualifiers
     source          1..8520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..726
                     /codon_start=1
                     /product="monomeric photoconvertible Kikume green-to-red 
                     fluorescent protein"
                     /label=mKikGR1
                     /translation="MVSVITSEMKIELRMEGSVNGHKFVITGKGSGRPYEGTQTVDLTV
                     IEGGPLPFAFDILTTAFHYGNRVFVEYPEEIVDYFKQLFPEGYSWERSMSYEDGGICLA
                     TNNITMKKDGSNTFVNEIRFDGTNFPANGPVMQRKTVKWEPSTEKMYVRDGVLKGDVEM
                     ALLLEGGGHYRCDFRTTYKAKKVVQLPDYHYVDHQMEITSHDKDYNKVKAYEHAKAYSG
                     TYRGAKYEFEA"
     regulatory      775..784
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagtgtga ttacatcaga aatgaagatc
       61 gagctgcgta tggaaggctc tgtaaacggg cacaagttcg tgattacagg gaaaggaagt
      121 ggcagacctt acgagggaac acagactgtg gacctgacag tcatagaggg cggacctctt
      181 ccttttgctt tcgatatcct gacaacagca ttccattacg gcaaccgggt atttgtcgaa
      241 tacccagaag aaatagtaga ctacttcaag cagttgtttc ctgagggtta ttcttgggaa
      301 cgaagcatga gttacgaaga cgggggaatt tgcctcgcca caaacaatat aacgatgaag
      361 aaagacggca gcaacacttt tgtcaatgaa attcgatttg atggtacaaa ctttcctgcc
      421 aatggtccag ttatgcagag gaagaccgtc aaatgggagc catccactga gaaaatgtat
      481 gtgcgtgatg gagtgctgaa gggtgatgtt gaaatggctc tgttgcttga aggaggtggc
      541 cattaccgat gtgacttcag aactacttac aaagcaaaga aggttgtcca gttgccagac
      601 tatcactacg tggatcatca aatggagata acaagccatg acaaggatta caacaaggtt
      661 aaggcgtatg aacatgctaa ggcttattcc gggacctaca ggggcgccaa gtatgaattt
      721 gaagcctccg gactcagatc tcgagctcaa gctagcttcg aattcgcaga agctgccacc
      781 atggttgcgc tttcgctgaa gattagcatt gggaatgtgg tgaagacgat gcaatttgag
      841 ccatctacca tggtgtacga cgcctgccgc atgattcgtg agcggatccc agaggccctg
      901 gctggccctc ccaacgactt tgggctcttt ctgtcagatg atgaccccaa aaaaggcatc
      961 tggctagagg ctgggaaagc tttggactac tacatgctcc gaaatgggga caccatggag
     1021 tacagaaaaa agcagagacc cctgaagata cggatgttag acggaacggt gaagactatc
     1081 atggtggatg actccaagac cgtcacagac atgctcatga ccatttgtgc ccgaattggt
     1141 atcaccaacc atgatgagta ttcactggtt cgagagctga tggaagaaaa gaaagatgag
     1201 gggacaggga ccctgagaaa agacaagacc ctactgcgag atgaaaagaa gatggagaag
     1261 ctaaagcaga agttgcacac agacgatgag ttgaactggc tggaccatgg gcggacactg
     1321 agggaacagg gagtggaaga gcatgagacg ttgctgctgc ggagaaagtt cttctactca
     1381 gaccagaatg tggattcccg agaccctgta cagctgaacc ttctctatgt gcaggcacga
     1441 gatgacatcc tgaatggctc ccatcctgtc tcctttgaca aggcctgtga atttgcaggc
     1501 ttccagtgcc agatccagtt tggacctcac aatgaacaga agcacaaggc tggcttcctt
     1561 gacctgaagg acttcctgcc caaggagtat gtgaagcaga agggagagcg taagatcttt
     1621 caggcacaca agaattgtgg gcagatgagt gaaattgagg ccaaggtacg ctacgtgaag
     1681 ctggcccgtt ccctcaagac ttacggtgtc tccttcttcc tagtcaagga aaagatgaag
     1741 gggaagaata aactggtgcc caggctgttg ggcatcacta aggagtgtgt gatgcgtgtg
     1801 gatgagaaga ccaaggaggt gattcaggaa tggagcctca ccaacatcaa acgctgggct
     1861 gcctctccca agagctttac tctggacttt ggagactatc aggatggcta ctactcagta
     1921 cagacgacag aaggcgagca gatcgcacag ctcattgctg gctacataga tatcatcctt
     1981 aagaagaaaa aaagcaagga ccattttggg ctggagggag atgaagagtc tactatgttg
     2041 gaggactcag tgtctcccaa aaagtcaaca gtccttcagc agcagtacaa ccgagtgggg
     2101 aaagtggagc acggctctgt ggctctgcca gccatcatgc gctctggagc ctctggtcct
     2161 gagaatttcc aggtggggag catgccacct gcccagcagc agatcaccag tggccagatg
     2221 caccgaggac acatgccacc tctgacttca gcccagcagg cgctcactgg aaccattaac
     2281 tccagcatgc aggctgtgca ggctgcccag gccactctgg atgactttga gactctaccc
     2341 cctcttggcc aggatgctgc ctccaaagct tggcgtaaga acaagatgga tgaatcaaag
     2401 catgagatcc actcccaggt agatgccatc acagctggca ctgcctctgt ggtaaacctg
     2461 acagcaggag atcctgcaga gacagactat acagcagtgg gctgtgcagt caccaccatc
     2521 tcttccaacc tgacggagat gtcccgtggg gtgaagctat tggctgccct gctggaggat
     2581 gaaggcggca atggccggcc ccttctgcaa gcagcaaagg gccttgcagg ggctgtgtcg
     2641 gaactgcttc gcagtgctca gcccgccagc gctgagcctc gtcagaacct gctgcaggca
     2701 gccgggaacg tgggccaggc cagtggggag ctgttgcagc aaattgggga aagtgacact
     2761 gacccccact tccaggatgt tctaatgcag ctagcaaacg cagtggcaag tgctgcagcc
     2821 gccctggtcc tcaaggccaa gagtgtggcc cagcgcacag aggattccgg gcttcagacc
     2881 caagtgattg ctgcagctac gcaatgtgct ctgtccactt cccagctggt ggcctgtacc
     2941 aaggtggtag cacctacaat cagctcacct gtgtgccaag aacagctagt cgaggctggg
     3001 cgactggtgg ccaaagctgt ggagggctgt gtgtctgcct cgcaagcagc tacagaagat
     3061 ggacagctgc ttcgaggggt aggagcagca gccacggctg tcacccaggc cctcaatgag
     3121 ctgctgcagc acgtgaaggc ccacgccacc ggagctgggc ctgccggtcg gtatgaccaa
     3181 gccactgata ccatcctcac cgtcactgaa aacatcttca gctccatggg tgatgctggg
     3241 gagatggtgc gccaggcccg catcctggcc caagccacct cagacctggt caatgctatc
     3301 aaggccgatg ctgaggggga gagtgatctg gagaactcta gaaagctcct gagtgctgcc
     3361 aagatcctcg ctgatgccac cgccaagatg gtggaggcgg ccaagggagc agccgcccac
     3421 cctgacagtg aggaacagca gcagcgactg cgtgaagcag ctgaggggct tcgcatggcc
     3481 accaatgcag ctgcgcagaa cgccatcaag aagaagttgg tgcagcgcct ggagcatgca
     3541 gccaagcaag ctgcagcctc tgcaacacag accattgctg cagcccaaca tgcagcctct
     3601 gcccccaagg cctctgcggg cccccagccc ctactggtgc agagctgtaa ggccgtggca
     3661 gagcagattc cgttgctggt gcagggtgtc cgaggaagtc aagctcagcc tgacagccct
     3721 agtgctcagc tcgccctcat tgctgccagc cagagcttcc tgcagccagg tgggaagatg
     3781 gtggcagcgg caaaagcctc agtgccaaca attcaggacc aggcttcagc gatgcagctg
     3841 agtcagtgcg caaagaacct aggcactgcc ctggccgagc tgcgcactgc tgctcagaag
     3901 gctcaggagg catgtggacc tttggagatg gattctgcac tgagtgttgt acaaaatcta
     3961 gagaaagacc tacaggaaat aaaagcagca gctcgagatg gcaagcttaa gcccttacct
     4021 ggggaaacaa tggagaagtg tacccaagat cttggcaaca gcaccaaagc ggtgagctct
     4081 gccatcgcca agctgctggg ggagattgcc cagggcaacg agaactatgc aggtattgca
     4141 gctcgggatg tagctggtgg actaaggtca ctagcccagg ctgcgcgggg tgtggctgcg
     4201 ctgacatcag atcctgcagt gcaggccatt gtgcttgaca cagccagcga cgttctggac
     4261 aaggccagca gcctcattga agaggcaaaa aaagcatctg gacacccagg ggacccagaa
     4321 agccagcaaa ggcttgctca ggtagctaaa gcagtgaccc aagccctgaa ccgctgtgtc
     4381 agctgcctgc ctggccaaag agacgtggat aatgccctac gagccgttgg agatgccagc
     4441 aagcgactcc tgagtgactt gcttcctcca agcacgggga catttcaaga agcacagagc
     4501 cgattgaatg aagctgcagc tgggttaaat caggccgcca cagaactggt gcaagcctcc
     4561 cgaggaactc ctcaggacct ggctcgggcc tccggccgat ttggacagga cttcagcacc
     4621 ttcctggaag ctggtgtgga gatggccgga caggccccga gccaggagga ccgagcccag
     4681 gtggtgtcca acctgaaggg catatccatg tcttcaagca aacttctcct tgccgctaag
     4741 gccttgtcca cagaccctgc ttctcccaac ctcaagagtc agctggctgc ggctgcccgg
     4801 gcagtgacgg acagcatcaa ccagctcatc accatgtgca cccagcaggc acctggccag
     4861 aaggagtgtg acaatgcact tcggcagcta gagacagtcc gagaactcct ggagaatcca
     4921 gtccagccca tcaacgacat gtcctacttc ggttgcttgg acagtgtcat ggagaactcc
     4981 aaggtcctag gtgaggccat gactggcatc tcccaaaatg ccaagaatgg aaatctgccg
     5041 gagtttggag atgccattgc cacagcctcc aaggctctct gtggcttcac ggaggcagcc
     5101 gcacaggcag catatctagt tggtgtctct gaccccaaca gccaagctgg acagcaagga
     5161 ctggtggaac ccacacagtt tgcccgtgca aaccaggcaa ttcagatggc ctgtcagagt
     5221 ctgggggagc ctggctgtac ccaggcccag gtgttatctg cagccactat tgtagccaaa
     5281 cacacatctg cattgtgtaa cagctgtcgc ctggcttccg ctaggactgc caatcccact
     5341 gccaagcgcc agtttgtaca gtcagccaag gaggtggcca acagtacagc caatcttgtc
     5401 aagaccatca aggcactaga tggggacttc acagaagaga accgtgccca gtgccgagca
     5461 gccacagccc ctctgctgga agctgtggat aacctgagtg cctttgcctc caaccctgag
     5521 ttctccagcg tccctgccca gatcagccct gagggcagag cagccatgga gcccattgta
     5581 atctctgcta agacaatgtt ggagagtgct ggagggctca tccagacagc tcgggcgtta
     5641 gcagtcaatc cccgagaccc cccacgttgg tctgtgctag ctggccactc tcggactgtc
     5701 tcagattcca tcaagaaact tattacaagc atgagggaca aagccccagg gcagctggag
     5761 tgtgagacag ccattgcggc tctgaacagc tgcttgcggg acctagatca ggcttcgctt
     5821 gccgctgtca gccagcagct tgctccccgt gaaggaatct ctcaagaggc tttgcacacc
     5881 cagatgctca ctgcagtgca agaaatttct catctcattg agccgctggc cagcgctgct
     5941 cgggctgaag cctcccagct gggacacaag gtgtcccaaa tggcccagta ctttgaacca
     6001 ctcaccctgg ctgcagtggg tgctgcgtct aagaccctga gccacccgca gcagatggca
     6061 cttctggacc agactaaaac gttggcagag tctgccttgc agttgctata tactgccaag
     6121 gaggctggtg gtaaccccaa gcaagcagca cacacccagg aagccctgga ggaggctgtg
     6181 cagatgatga cagaggccgt agaagacctg acgacgaccc tcaacgaagc agccagtgct
     6241 gcaggagtcg ttggcggcat ggtggactct atcactcagg ccatcaacca gctagatgaa
     6301 ggacctatgg gtgacccaga aggctcattc gtagattacc agacaaccat ggtgaggaca
     6361 gccaaggcca ttgctgtcac tgttcaggag atggtaacca agtcaaacac cagccctgaa
     6421 gagctaggcc ctcttgccaa ccagctgacc agtgactatg gccgactggc ctcacaagcc
     6481 aagcctgcag ctgtggctgc tgaaaatgaa gagataggcg ctcacatcaa acaccgagta
     6541 caggagctgg gccatggctg ctctgctctg gtcaccaagg caggtgcctt gcagtgtagc
     6601 cccagtgatg tctacaccaa gaaggagctc atagagtgtg cccgcagagt gtcagagaag
     6661 gtctcccatg ttctggctgc actccaggct gggaatcgtg gtacccaggc ctgcattaca
     6721 gcagccagtg ctgtgtctgg tatcattgct gacctcgaca ccaccatcat gttcgctact
     6781 gctggcacac ttaaccgtga gggtgctgaa acttttgctg accaccggga gggtatctta
     6841 aagacagcaa aggttcttgt ggaggacacc aaggtcctag tgcagaatgc agctgggagc
     6901 caggagaagt tggcacaagc cgcccagtcc tccgtggcca ccattacccg cctcgctgat
     6961 gtggtcaagc tcggtgcagc cagcctagga gccgaagacc ctgaaactca ggtggtgctg
     7021 atcaatgcag taaaggacgt agccaaggcc ctgggtgacc tcatcagcgc tacgaaggct
     7081 gcagcgggca aagttgggga tgaccctgca gtgtggcagc tcaagaactc tgccaaggtg
     7141 atggtgacca atgtgacatc attgctcaag acagtgaagg ctgtggaaga tgaggccacc
     7201 aaaggcacac gggccctaga ggcaaccaca gagcacatac gtcaggaact ggcggtcttc
     7261 tgttccccag agccacctgc caagacctct acccctgaag acttcatccg aatgaccaag
     7321 ggtattacta tggcaacagc caaagccgtt gctgctggca attcctgtcg acaggaagat
     7381 gtcattgcca cagccaatct gagccgacgg gctattgcgg acatgcttcg ggcttgcaag
     7441 gaagcagctt tccacccaga agtggcgcct gatgtacggc tccgagccct gcactatggc
     7501 cgggagtgtg ccaatggtta cctggaactg ctggaccacg tgctgctgac ccttcagaag
     7561 ccaaacccag acctgaagca gcagctaact ggacactcaa agcgggttgc tggctctgtg
     7621 actgagctca tccaggctgc tgaagccatg aaaggaacag agtgggtgga cccagaggac
     7681 cctactgtca ttgctgagaa tgagctcttg ggagccgcag ccgccatcga ggctgcagcc
     7741 aagaagctgg agcagctgaa gcccagggcc aaacccaagg aggcggatga gtccttgaac
     7801 tttgaggaac aaatcctaga agctgccaag tccatcgctg cagccaccag tgcactggta
     7861 aaggctgcgt cagcagccca gagggagctg gtggctcaag gaaaggtggg cgccattcca
     7921 gccaatgcac tggatgatgg gcagtggtcg cagggcctca tttccgctgc ccgtatggtg
     7981 gctgcggcca ccaacaatct gtgtgaagca gctaacgcag ctgtccaggg ccatgctagc
     8041 caggagaaac tcatttcctc agccaagcaa gtagctgcct ccacagctca gctcctggta
     8101 gcttgcaagg tcaaagcaga tcaggactct gaggcaatga aacggctcca ggctgctggc
     8161 aatgcagtga agagggcctc agataacctg gtaaaggcgg cccagaaagc tgcagccttc
     8221 gaagaccagg agaatgagac ggtggtggtg aaggagaaaa tggttggggg cattgcccag
     8281 attatcgcag cacaggaaga gatgcttcgg aaggaacgag agctggaaga ggctcggaaa
     8341 aagctcgccc agatccggca gcagcagtac aagttcttgc cttcagagct tcgagacgag
     8401 cactaaaaaa gccctgtgta tttaatgcag acccagccca gagactgtgc ctgccactac
     8461 caaagccttc tgggctgctc aggacccaag ctgcccaacc ccagcactcc cccaaagtgc
//
