LOCUS       sequence-88464-e         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos-A69V-Mito 7
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..93
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             127..807
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFVEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gacgtcagat ccgctagcat gtccgtcctg acgccgctgc tgctgcgggg cttgacaggc
       61 tcggcccggc ggctcccagt gccgcgcgcc aagatccatt cgttggggga tccaccggtc
      121 gccaccatga gtgcgattaa gccagacatg aagatcaacc tccgtatgga aggcaacgta
      181 aacgggcacc actttgtgat cgacggagat ggtacaggca agccttttga gggaaaacag
      241 agtatggatc ttgaagtcaa agagggcgga cctctgcctt ttgcctttga tatcctgacc
      301 actgcattcc attacggcaa cagggtattc gtcgaatatc cagaccacat acaagactat
      361 tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa gcttgacttt cgaagacggg
      421 ggcatttgca ttgccagaaa cgacataaca atggaagggg acactttcta taataaagtt
      481 cgatttcacg gtacaaactt tcccgccaat ggtccagtta tgcagaagaa gacgctgaaa
      541 tgggagccct ccactgagaa aatgtatgtg cgtgatggag tgctgacggg tgatattcac
      601 atggctttgt tgcttgaagg aaatgcccat taccgatgtg acttcagaac tacttacaaa
      661 gctaaggaga agggtgtcaa gttaccaggc taccactttg tggaccactg cattgagatt
      721 ttaagccatg acaaagatta caacaaggtt aagctgtatg agcatgctgt tgctcattct
      781 ggattgcctg acaatgccag acgataagcg gccgcgactc tagatcataa tcagccatac
      841 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      901 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      961 ataaagcaat agca
//
